FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_9420_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9420_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278497
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC17830.6402223363267827No Hit
GCTATAACTATTTTAGTGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA11560.4150852612415933No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAG9860.354043311059006No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG8940.3210088439013706No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTT8520.3059278915033196No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA8170.2933604311716105No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC7500.26930272139376726No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT7110.25529897988129135No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC6910.24811757397745757No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAGTGCATGTGTC6890.2473994333870742No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC6260.22477800478999774No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC6230.22370079390442266No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG5950.21364682563905535No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT5880.21113333357271352No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA5800.20826077121118003No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC5730.2057472791448382No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC5610.2014384356025379No Hit
TAGTAGCACTGCTATAACTATTTTAGTGCATGTGTCAGGAAGGAGTTGAA5490.19712959206023764No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT5370.19282074851793735No Hit
GTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGA5290.18994818615640383No Hit
CACTTGGAGGTGTGTTTCATGTATTCAAGTCAGTATTCAATAGCCTGTAT5070.18204863966218668No Hit
GTATGGGGAGGATCTGAAAATTGAAACCAACAAATTTGCAGCAATATGCA4370.1569137189987684No Hit
ATTTTAGTGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA4340.1558365081131933No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG4280.1536820863420432No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG4240.15224580516127642No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA4160.1493732427997429No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA4010.14398718837186755No Hit
CCCTAAGGGGAAGAGGCAATACTCTCGGTCTAGACATCAAATCAGCCACC4000.1436281180766759No Hit
GGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAG3980.1429099774862925No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG3930.14111462601033403No Hit
GACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCT3860.1386011339439922No Hit
CATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTTAGAGACTCGAACT3780.1357285715824587No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG3770.13536950128726702No Hit
GTTCTTATCTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAA3770.13536950128726702No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA3750.13465136069688363No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3730.13393322010650024No Hit
ATACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGC3690.1324969389257335No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC3690.1324969389257335No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTTTT3650.13106065774496672No Hit
CTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAATGTTATTT3630.13034251715458334No Hit
ATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTTTTAAC3610.12962437656419998No Hit
GTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCCGGAAACAAGT3590.1289062359738166No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA3560.1278290250882415No Hit
CAAGTTGTAGACCAAAAACTGAGTGATGCCCCATTCCTCGATCGGCTTCG3490.1253155330218997No Hit
GTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAACTTCTGACCTA3460.12423832213632462No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA3440.12352018154594124No Hit
CCCTAGAAGATCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA3360.12064761918440772No Hit
CTATTTTAGTGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCA3350.12028854888921604No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG3340.11992947859402435No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG3230.11597970534691576No Hit
CTCAATATCAGTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC3220.11562063505172408No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC3190.11454342416614902No Hit
GTAGTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTT3160.11346621328057394No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGA3080.11059365091904041No Hit
GTCTAGACATCAAATCAGCCACCCATGTTGGAAAGCAAATCGTAGAAAAG3030.10879829944308197No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT3020.10843922914789028No Hit
CTATTGAGGAATTGTCAAGAAACTGGTTCATGCTAATGCCCAAGCAGAAA2950.10592573708154845No Hit
GTATTATTATCTCCTGAGGAGGTCAGTGAAACACAGGGAACTGAAAGACT2950.10592573708154845No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA2920.10484852619597339No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2890.10377131531039832No Hit
ACAAATAGTAGCACTGCTATAACTATTTTAGTGCATGTGTCAGGAAGGAG2880.10341224501520661No Hit
CCATAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA2880.10341224501520661No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT2870.10305317472001493No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGCTTGTCCCT2840.10197596383443987No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2820.1012578232440565No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT2790.10018061235848141No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC1050.096.580723
TCTAATA301.6044949E-596.580723
AGCGAAA1150.094.481131
TAAACTA406.1382343E-790.5444265
TAGATGG353.4419543E-582.813218
TTCTAAT353.448094E-582.783462
TAACCAT353.448094E-582.783465
TATTAAC353.448094E-582.783462
TTAACCA353.448094E-582.783464
CAGTACG2050.074.228919
CTTGTGG300.001941108572.435543
ATTAACC451.19761185E-464.3871463
GTACTGA702.3369284E-762.1099056
GAGCGAA350.003576383762.0875971
CAACTCT954.5838533E-1060.998354
ACTATTC606.814136E-660.3846328
TCTATGC1700.059.6527943
TCCAACT1007.221388E-1057.9484332
CTATTAA502.0165612E-457.9484331
AAGCTAA502.0165612E-457.9484335
Produced by FastQC (version 0.11.5)