FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_9332_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9332_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1227565
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTAGTAGTGGTCGTAATTAGTTTGTATGTGCTTCTGTCATTTTGCTCA45060.36706813895801854No Hit
GATACAGACTGCGCATATCTGCGCATTAAAAATTCATGTAAAGTGAGTGA44530.3627506486418235No Hit
GTCCTGGATCTCTGACCATGTTTCTGATGAACGAAGGACCGACACTTGGG40330.32853657443801343No Hit
AGTAGGTACTGCTGTCAATAAACATAAGAGCTAAAGGAATGAACAATGTC40090.32658148448351004No Hit
CATATGGTGTGATTCAAGGTTTTTTTTCAGCTTGGTGGGATTTGGGCTGG38260.3116739235804214No Hit
GTATTTAATGAGGGATTTTTAATGAATTAATAAAAGAGAGACGTGTTGTA35130.2861762920904392No Hit
GGTTTAAGATAAGGCCCCCGCCCGCATGAATTTTTTATCCAGACAAGGCA33950.27656376648079734No Hit
GCGCATATCTGCGCATTAAAAATTCATGTAAAGTGAGTGAGAGAGAGACA32510.2648332267537768No Hit
GTATAATAAAGATACAGACTGCGCATATCTGCGCATTAAAAATTCATGTA31750.2586421085645159No Hit
GTGTAATGTAGTGTAGTGTGGTATGAGTGTGTGTGTAAGGGTTTGTGTTG30950.2521251420495045No Hit
CACATATGGTGTGATTCAAGGTTTTTTTTCAGCTTGGTGGGATTTGGGCT27670.22540557933795766No Hit
GCCTTAGTTAAGGATAATCCAGCCTAAGTATAATAAAGATACAGACTGCG24260.19762700956772145No Hit
AAACAAGGACGAGTAGGTACTGCTGTCAATAAACATAAGAGCTAAAGGAA22960.1870369389808279No Hit
CCCTTGAAGAGGTGATTAATGTTGATCACCAGCTAGAGCTTATTCCATTA21970.17897219291850125No Hit
GTACATGCATGTCCATGTCATACATAACCATAATTGACACCGGGAAAAAC21710.17685417880112256No Hit
TAGTTATAGTTATTAGTTATTTTTGCAACTTTTGTAAGACTCACGTTACT21370.1740844680322427No Hit
GTGTAATAATTATTACTAGTTGTAGTAGTGGTCGTAATTAGTTTGTATGT21370.1740844680322427No Hit
GTAGTGTAGTGTGGTATGAGTGTGTGTGTAAGGGTTTGTGTTGTGTTGTT20400.16618264613279132No Hit
GTATGAGTGTGTGTGTAAGGGTTTGTGTTGTGTTGTTAGATAATACCTGA19860.1617836937351586No Hit
CTTTTTGACTGGTCACTGTATTTAATGAGGGATTTTTAATGAATTAATAA19370.15779205174471414No Hit
CTCTTGTGGTGTTTATGCAAAAGCCGGTCACGTGAAGTCTGCAAATGTAC19310.15730327925608825No Hit
GTGTAAGGGTTTGTGTTGTGTTGTTAGATAATACCTGATCTATTTACCCT19070.15534818930158487No Hit
ATATTAAATTCTCCCTCCAGTCGACACCACCCGTGTGATCGTCAGGGAAG18950.15437064432433312No Hit
ATGTAGTGTAGTGTGGTATGAGTGTGTGTGTAAGGGTTTGTGTTGTGTTG18800.15314871310276848No Hit
GTAGTAATAAGTGTAATAATTATTACTAGTTGTAGTAGTGGTCGTAATTA18400.14989022984526276No Hit
GTTTAAACACCGGGTGGTGCTGTGGGTCCACGTTTAGCTCCCCCTTGGTC18220.14842391237938518No Hit
CTGTTTACACTTCCCTTGAAGAGGTGATTAATGTTGATCACCAGCTAGAG17720.14435080830750305No Hit
AGTGAATGGTGTGCCCTCTCCGCTCTGTGTCCACAGATGCTGCAGCAGCA17310.1410108629685597No Hit
GGAATGAACAATGTCAATGATTTGACGGGGAAAACTCACTCTGATGTAGT16050.1307466407074167No Hit
GTTAAGGATAATCCAGCCTAAGTATAATAAAGATACAGACTGCGCATATC16030.13058371654454143No Hit
GAACACATATGGTGTGATTCAAGGTTTTTTTTCAGCTTGGTGGGATTTGG15960.13001348197447793No Hit
CTATTTACCCTGTTGCCAACTCAGTACATGCATGTCCATGTCATACATAA15870.12928032324153915No Hit
GTTTAGTGTAATGTAGTGTAGTGTGGTATGAGTGTGTGTGTAAGGGTTTG15720.12805839201997452No Hit
CCCTGCATCTGCGCCACAGAGACGACTGCCACCCTGCATCTGCGCCACAG15610.12716230912416043No Hit
GTGTAAGAGGACCAGAGAGTTGTGCTCTACCGTTTTTCCCGGTGTCAATT14840.12088972885346194No Hit
CTCTAGTTGTGTGCACGTATCCTAATTGTCATTTTGCAAATTAGTCATCG14200.1156761556414528No Hit
GATGCAGGGTGGCAGTCGTCTCTGTGGCGCAGATGCAGGGTGGCAGTCGT14180.1155132314785775No Hit
GTGTCCACAGATGCTGCAGCAGCAGGAGTGCAAGCTCCTCTACCCTAGGA14000.11404691401269995No Hit
TACCTGGAGTGAACACTCAGCAAGGGCCAAAGAGAACCTCAAAACACTGA13930.11347667944263645No Hit
GTACTGCTGTCAATAAACATAAGAGCTAAAGGAATGAACAATGTCAATGA13790.11233621030250944No Hit
GGTACAGGGAGGGCAGATGGTAGAGGTGCAGGGGGATCAGCAGCACAGTC13320.10850749247494024No Hit
CTCCAGGCCTGTTCCTGGTGAGCAGCGGCCAGGCAGCCACCGGGGGGTTG13140.10704117500906266No Hit
GCATATCACATATCACATACGATTGGAAAAAATATTTTACACTAATCTCA13100.10671532668331209No Hit
CATTAAATACAGTGACCAGTCAAAAAGAGGCTGTAATGTCTCTCTCTCAC13050.10630801627612387No Hit
GAGTAAGGAGTGTGCTGCAACAAGCTCATACCTGGAGTGAACACTCAGCA13040.10622655419468624No Hit
GTATTGGAGTGAGCATTTTACCTGGAGAGGCCTCTTCATAGGGCCCCAGC12850.104678774647371No Hit
GGCTCAGGTTGTGCATGGGTCTCAATTTTTTGTGGAAGCACAGACCTTAC12630.10288660885574288No Hit
ATTAAATACAGTGACCAGTCAAAAAGAGGCTGTAATGTCTCTCTCTCACT12410.10109444306411472No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGAA100.0070781144145.08862145
AGCGAAA14300.0103.823411
CGGCTCC1250.087.05317145
GCGAAAG18750.079.1826552
GCGACCC851.8189894E-1276.81162145
TGGTCCC1700.072.54431145
CGGTAGG607.999188E-872.544304145
CGAAGCC502.2981949E-672.544304145
GAAAGCA21200.070.034684
AAGGACG6050.067.039045
GTCGACC651.3924182E-766.963974145
CGAAAGC22450.066.457823
GAGCGAA4100.065.357491
ACGAGTA6300.063.2420279
GTACTGG350.003558073762.180836145
AGGCTAG350.003578822662.0896386
ACCCAGG851.2993041E-859.742374145
CGCTACC753.7460632E-758.035442145
TACCTTT400.006036601554.408234145
TCTAGTG3900.053.8531232
Produced by FastQC (version 0.11.5)