FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_9332_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9332_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1235491
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTAGTAGTGGTCGTAATTAGTTTGTATGTGCTTCTGTCATTTTGCTCA45230.3660892713908883No Hit
GATACAGACTGCGCATATCTGCGCATTAAAAATTCATGTAAAGTGAGTGA44650.3613947815079187No Hit
GTCCTGGATCTCTGACCATGTTTCTGATGAACGAAGGACCGACACTTGGG40470.3275620785582412No Hit
AGTAGGTACTGCTGTCAATAAACATAAGAGCTAAAGGAATGAACAATGTC40200.32537671257823814No Hit
CATATGGTGTGATTCAAGGTTTTTTTTCAGCTTGGTGGGATTTGGGCTGG38390.31072666656414333No Hit
GTATTTAATGAGGGATTTTTAATGAATTAATAAAAGAGAGACGTGTTGTA35250.2853116696115148No Hit
GGTTTAAGATAAGGCCCCCGCCCGCATGAATTTTTTATCCAGACAAGGCA34070.2757608108840938No Hit
GCGCATATCTGCGCATTAAAAATTCATGTAAAGTGAGTGAGAGAGAGACA32680.264510223061115No Hit
GTATAATAAAGATACAGACTGCGCATATCTGCGCATTAAAAATTCATGTA31920.25835882252480996No Hit
GTGTAATGTAGTGTAGTGTGGTATGAGTGTGTGTGTAAGGGTTTGTGTTG31030.2511552087388738No Hit
CACATATGGTGTGATTCAAGGTTTTTTTTCAGCTTGGTGGGATTTGGGCT27790.22493081697883677No Hit
GCCTTAGTTAAGGATAATCCAGCCTAAGTATAATAAAGATACAGACTGCG24320.19684481716175997No Hit
AAACAAGGACGAGTAGGTACTGCTGTCAATAAACATAAGAGCTAAAGGAA22990.18607986622322625No Hit
CCCTTGAAGAGGTGATTAATGTTGATCACCAGCTAGAGCTTATTCCATTA22030.17830967607210413No Hit
GTACATGCATGTCCATGTCATACATAACCATAATTGACACCGGGAAAAAC21780.17628618905358276No Hit
TAGTTATAGTTATTAGTTATTTTTGCAACTTTTGTAAGACTCACGTTACT21460.17369612566987538No Hit
GTGTAATAATTATTACTAGTTGTAGTAGTGGTCGTAATTAGTTTGTATGT21440.17353424670839365No Hit
GTAGTGTAGTGTGGTATGAGTGTGTGTGTAAGGGTTTGTGTTGTGTTGTT20500.1659259355187533No Hit
GTATGAGTGTGTGTGTAAGGGTTTGTGTTGTGTTGTTAGATAATACCTGA19900.16106956667430197No Hit
CTTTTTGACTGGTCACTGTATTTAATGAGGGATTTTTAATGAATTAATAA19420.1571844715987409No Hit
CTCTTGTGGTGTTTATGCAAAAGCCGGTCACGTGAAGTCTGCAAATGTAC19350.15661789523355493No Hit
GTGTAAGGGTTTGTGTTGTGTTGTTAGATAATACCTGATCTATTTACCCT19130.1548372266572561No Hit
ATATTAAATTCTCCCTCCAGTCGACACCACCCGTGTGATCGTCAGGGAAG19050.15418971081132926No Hit
ATGTAGTGTAGTGTGGTATGAGTGTGTGTGTAAGGGTTTGTGTTGTGTTG18910.1530565580809573No Hit
GTAGTAATAAGTGTAATAATTATTACTAGTTGTAGTAGTGGTCGTAATTA18480.14957616040910052No Hit
GTTTAAACACCGGGTGGTGCTGTGGGTCCACGTTTAGCTCCCCCTTGGTC18240.14763361287131999No Hit
CTGTTTACACTTCCCTTGAAGAGGTGATTAATGTTGATCACCAGCTAGAG17770.1438294572764998No Hit
AGTGAATGGTGTGCCCTCTCCGCTCTGTGTCCACAGATGCTGCAGCAGCA17360.1405109385661247No Hit
GGAATGAACAATGTCAATGATTTGACGGGGAAAACTCACTCTGATGTAGT16120.13047444295425867No Hit
GTTAAGGATAATCCAGCCTAAGTATAATAAAGATACAGACTGCGCATATC16090.1302316245120361No Hit
GAACACATATGGTGTGATTCAAGGTTTTTTTTCAGCTTGGTGGGATTTGG15980.1293412902238867No Hit
CTATTTACCCTGTTGCCAACTCAGTACATGCATGTCCATGTCATACATAA15940.12901753230092328No Hit
GTTTAGTGTAATGTAGTGTAGTGTGGTATGAGTGTGTGTGTAAGGGTTTG15750.12747968216684705No Hit
CCCTGCATCTGCGCCACAGAGACGACTGCCACCCTGCATCTGCGCCACAG15650.12667028735943847No Hit
GTGTAAGAGGACCAGAGAGTTGTGCTCTACCGTTTTTCCCGGTGTCAATT14860.12027606838091091No Hit
CTCTAGTTGTGTGCACGTATCCTAATTGTCATTTTGCAAATTAGTCATCG14260.1154196995364596No Hit
GATGCAGGGTGGCAGTCGTCTCTGTGGCGCAGATGCAGGGTGGCAGTCGT14240.11525782057497788No Hit
GTGTCCACAGATGCTGCAGCAGCAGGAGTGCAAGCTCCTCTACCCTAGGA14040.11363903096016077No Hit
TACCTGGAGTGAACACTCAGCAAGGGCCAAAGAGAACCTCAAAACACTGA13980.11315339407571565No Hit
GTACTGCTGTCAATAAACATAAGAGCTAAAGGAATGAACAATGTCAATGA13840.11202024134534366No Hit
GGTACAGGGAGGGCAGATGGTAGAGGTGCAGGGGGATCAGCAGCACAGTC13360.10813514626978261No Hit
CTCCAGGCCTGTTCCTGGTGAGCAGCGGCCAGGCAGCCACCGGGGGGTTG13200.10684011457792893No Hit
GCATATCACATATCACATACGATTGGAAAAAATATTTTACACTAATCTCA13150.10643541717422465No Hit
CATTAAATACAGTGACCAGTCAAAAAGAGGCTGTAATGTCTCTCTCTCAC13080.10586884080903868No Hit
GAGTAAGGAGTGTGCTGCAACAAGCTCATACCTGGAGTGAACACTCAGCA13070.10578790132829781No Hit
GTATTGGAGTGAGCATTTTACCTGGAGAGGCCTCTTCATAGGGCCCCAGC12870.1041691117134807No Hit
GGCTCAGGTTGTGCATGGGTCTCAATTTTTTGTGGAAGCACAGACCTTAC12660.10246938261792277No Hit
ATTAAATACAGTGACCAGTCAAAAAGAGGCTGTAATGTCTCTCTCTCACT12410.10044589559940137No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTCC1600.0122.45004145
AGCGAAA13100.0106.7110751
GTCGACC651.4551915E-1189.3083145
GCGAAAG16850.082.962322
TAGTGCA1800.080.488484
TCTTAGA851.8189894E-1276.691472
CTTAGAC851.8189894E-1276.691473
TATAGGC1750.074.500292
GGCTAGC300.001940387772.457237
AGGCTAG300.00194101172.451366
ATAGGCC1800.072.430843
GAAAGCA19500.072.0681464
CGAAAGC20500.069.2509463
GCCTGTA2050.067.155487
AAGGACG6200.066.600425
CGCTACC554.0412815E-665.966354145
GAGCGAA3350.062.701321
GTATAGG2200.062.5539051
GGTAGCG350.003554451762.196846145
ACGAGTA6700.060.5612649
Produced by FastQC (version 0.11.5)