FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_9105_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9105_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748070
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG70970.4059906067834812No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC50260.28751709027670513No Hit
ATTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT47900.27401648675396295No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG44280.2533079338928075No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC39740.22733643389566777No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG35550.2033671420480873No Hit
CTCCAATTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT34480.1972461057051491No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT32650.18677741738031087No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC28900.165325187206462No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG28780.1646387158408988No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG28700.1641810682638567No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA27170.15542855835292635No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC25810.14764854954321052No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA25000.14301486782565914No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC24560.14049780615192756No Hit
GTCTATGAGACCTGTGCTGGGAATCAGCAATCTGCTCACATGTTGCACAC24120.13798074447819594No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC23420.1339763281790775No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA23370.13369029844342617No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG22790.1303723535098709No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA22480.1285989691488327No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT22130.1265967609992735No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA22020.12596749558084058No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA21900.12528102421527743No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT21610.12362205174849977No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT21580.12345043390710897No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG21560.12333602201284846No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA20950.11984645923790237No Hit
CTCATAGACAGATGGTGGCAACAACCAATCCATTAATAAAACATGAGAAC20740.11864513434816683No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA20660.11818748677112473No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC20590.11778704514121287No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA20390.11664292619860761No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT20030.11458351210191813No Hit
GTATTATTATCTCCTGAGGAGGTCAGTGAAACACAGGGAACTGAAAGACT19980.1142974823662668No Hit
GATAATGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT19660.11246689205809834No Hit
TGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA18980.10857688765324043No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG18770.1073755627635049No Hit
TCCCTGTACACATTGTGGTCATAAGTTTCATTTCTTATTGATTCTATGCA18710.10703232708072331No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA18490.1057737962438575No Hit
CTCATTATCAGTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC18430.10543056056107591No Hit
CTAATAGATGCTCTATTGGGAGACCCGCAGTGTGATGGCTTTCAAAATAA18420.10537335461394566No Hit
GTTCTTATCTCTTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAA17770.10165496805047852No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT17600.10068246694926405No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACC151.2259478E-4145.12494145
GGCGTGC100.0071082874144.884088
GCTGACC1500.0111.26244145
GGCCGCC203.8533195E-4108.84371145
GCTGCCC950.091.65785145
GCCGGCC406.088103E-790.703094145
ATACGCC406.088103E-790.703094145
CGACTCC900.088.68746145
TCACGCC750.087.07496145
CGAATAA1400.082.928535145
TGACGCC900.080.62497145
AGCGTAA554.370122E-879.159065145
AGCGAAA3350.077.8169861
CGAAAGC3550.077.514763
GGTCGTT554.0828963E-665.854527
AGGTCGT554.083591E-665.852636
GTGCCCC451.1890281E-464.49997145
AAACTAT2200.062.566
GAAGCCC606.773036E-660.468727145
AGGGTGT9200.059.846
Produced by FastQC (version 0.11.5)