FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_895_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_895_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1750473
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT92270.5271146712917023No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC78170.44656501414189187No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA63430.36235920234131No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAA55260.3156861031275547No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAACTGTGT50310.2874080320004936No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG46540.2658709960107925No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT45760.2614150575301647No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT45540.260158254368962No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG43760.24998957424650367No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT37890.2164557808089585No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC35630.2035449846984215No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG35560.20314509278349335No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAGCA33740.19274790299536182No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT33620.19206237399834214No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC31470.17977997946840654No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA29650.16938278968027498No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT29260.16715482043996108No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA27700.15824294347870546No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA26050.14881691976968509No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT25290.14447523612189392No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT24870.1420758846323251No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA23770.13579186882631153No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT23690.13533484949496508No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT23440.13390666408450744No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG23010.1314501851785203No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA21320.12179565180382673No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC20860.11916779064858471No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT20720.11836800681872843No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT20610.11773960523812706No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC20280.11585440049632299No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA19850.11339792159033588No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT19440.11105569751718536No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT19380.11071293301867552No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT19320.11037016852016568No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC19130.1092847476082179No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA18010.1028864769693677No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT17860.10202956572309312No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTCTATCACATGTAT17730.10128690930965516No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT17620.10065850772905381No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTC100.0070781405145.0902145
GGGTAGA100.0071157515144.833251
AGCACGC10750.0123.950311
GCACGCA10950.0123.0090562
CACGCAC11100.0121.999173
GGCACCC1100.0112.11515145
GAAACAC8950.0106.807454
CGCACCC352.7524038E-7103.63586145
GAACGCC501.9463187E-10101.56313145
AAACACG9550.0100.097045
ATAAGCC800.099.74951145
CGGTGCA800.099.572852
AGCCACC607.2759576E-1296.72681145
GCGAGAA451.0873919E-896.72679145
GCAGCCC451.0873919E-896.72679145
GCTGCCC651.6370905E-1189.28628145
ACGCACT15900.088.359914
CGCACTT16000.088.260285
TAGAAAC13200.087.229112
TGACGCC259.364768E-487.054115145
Produced by FastQC (version 0.11.5)