FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_874_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_874_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1417548
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAGGGGGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG124840.8806756455513323No Hit
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC42380.2989669485618829No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT38140.2690561448360126No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT30120.21247957741113527No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG29610.20888181564222164No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC27130.1913868172365239No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT24160.17043514575873267No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA23600.16648466224776867No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT22160.1563262760767184No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG21910.1545626673664666No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC21680.15294014735303496No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA21140.1491307525388911No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT21070.1486369421000206No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT21070.1486369421000206No Hit
GGTGCACTTGCCAGTTGCATGGGCCTCATATACAACAGGATGGGAACAGT20370.14369883771131559No Hit
GAATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCCTTATG20360.14362829336290553No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTATCACGATTCAAATG20270.14299339422721488No Hit
GGTCTATTCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGGCCACTT19560.13798474549009979No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA19440.13713821330917894No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC19370.13664440287030843No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTG18790.1325528306625243No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC18580.1310713993459128No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG18580.1310713993459128No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT18190.12832016975792002No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA18090.1276147262738193No Hit
CTTATGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAG17560.12387587580808553No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC17250.12168900100737329No Hit
CCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGC17150.12098355752327258No Hit
GTCCTACATTGTGGAAACATCTAATTCAGACAATGGAACGTGTTACCCAG17100.12063083578122222No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC17050.12027811403917187No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT17030.12013702534235172No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC16140.11385857833385536No Hit
TCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG16090.11350585659180501No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATGGATAAG16070.11336476789498486No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA16020.1130120461529345No Hit
CCTTCTCTTCCAGGACATACTTATGAGGATGTCAAAAATGCAATTGGGGT15960.11258878006247407No Hit
GTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGATCCAGC15680.11061353830699207No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC15580.10990809482289136No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTC15430.10884992959674028No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAT15310.10800339741581942No Hit
GCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGAT15300.10793285306740935No Hit
CTTTCAATCTGTGCCGTATTTCTTCAATTAACCACCTTATTTCCTCAAAT15290.10786230871899928No Hit
CAATATGGGTTAGCCACTCAATTCAAATTGGAAATCAAAGCCAGATTGAA15030.10602815566033742No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTATCCAAAATGCC15020.10595761131192735No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA14720.10384128085962521No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACG14720.10384128085962521No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGTTCCCGCCACTTCTCATTTC14570.10278311563347414No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCC14390.10151331736209285No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAG14360.10130168431686264No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCC100.007076803145.0984145
CGGTGCC100.007076803145.0984145
TGACGCC650.0122.775566145
GTACACC301.5943098E-596.73227145
CGGACCC406.094124E-790.6865145
CCTCGCC1050.089.82282145
AGTAGAA3450.088.168111
TAAGCCC259.3622925E-487.059044145
GTAGAAA7750.077.563571
CAGGTAC950.076.238273
CGAAAGC4500.074.035843
CATCGGG300.001930705672.5492145
TTCGCCC300.001930705672.5492145
CCGCACC502.2974582E-672.5492145
CTCAGCC300.001930705672.5492145
AGGTCGT1400.062.0928886
GCGAAAG5400.061.694362
GGTCGTT1300.061.296827
TCGGTCC502.0038E-458.039364145
AGCGTAA651.089491E-555.807076145
Produced by FastQC (version 0.11.5)