FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_753_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_753_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1956657
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA106270.5431202300658726No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC90040.46017263117654245No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAA78450.40093894842069916No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC74870.38264243554184507No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG60680.3101207825387894No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA54870.2804272797940569No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT47580.2431698555239881No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAACTGTGT47330.24189216607714079No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT47330.24189216607714079No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA41350.21132983450855208No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT38430.196406421769375No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT37680.19257335342883294No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG35600.18194297723106298No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA33500.17121038587754522No Hit
CCATATAAACAATAAAAATAGCTAGCATTAATGTTACAGCCAAACTAGAA32680.1670195644918859No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC32640.16681513418039032No Hit
CCTTTAATGCAGGAGAATTTTCTCTCCCCACTTTTGATTCATTGAACATT32330.1652307992662996No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT31400.16047779452402747No Hit
TTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTATACTG30580.15628697313836815No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC29950.15306719573231284No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG29770.15214725933058273No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG28620.1462698878750849No Hit
CTACAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAG28610.146218780297211No Hit
CAGTTATAGTGTCTATTTTGTTGCTTTAATAATCGAGGTCATCATAATCC28200.14412336960438135No Hit
GAACATTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTA28110.1436634014035163No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT27750.14182352860005612No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA26870.13732606174715342No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT26730.13661055565691893No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG26440.13512843589857598No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT26040.13308413278362025No Hit
CTTTTGATTCATTGAACATTACTGCTGCATCTTTAAATGATGATGGATTG25350.12955770991032153No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG24950.12751340679536577No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA24700.1262357173485184No Hit
CTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAACA24690.12618460977064452No Hit
AGTAGAAACAACGCACTTGAACTTTATGTTAAATCCAGCAATCATGCTAA24600.12572464156977947No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA24090.12311815509821088No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT23690.12107385198325511No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT23380.1194895170691644No Hit
CTCTTAAGGTCTGCCGCCACTGCCACTCCATGTGCTCCGTGAGATGTGTA23300.11908065644617323No Hit
GTATGCTGCCATTGCAAGAATCGCGTAAAGAATAGCCATCACTTCACCAG23240.11877401097892988No Hit
CCATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAA22660.11580977146224403No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT22620.11560534115074846No Hit
GCATTAATGTTACAGCCAAACTAGAAGCAGCAGTTGAGTAATAGAGCAGT22450.11473651232689225No Hit
ATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTAAT22210.1135099304579188No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA22150.11320328499067542No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT21950.11218113343319754No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA21510.10993240000674619No Hit
GATAAGAACCAGCCTTGATGGACTGGTTCCTGTTGTGCTTACCGTTTTAT21230.10850138782627716No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC21160.1081436347811599No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT20800.10630376197769972No Hit
CAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG20600.10528161042022183No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCAT20560.10507718010872626No Hit
CCATAGAGGCTCTTCATTTGGGTTTTGTTATCCGAATGGAACCCCCAAAC20440.10446388917423953No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC20280.10364616792825723No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT20220.10333952246101386No Hit
GTATTATTGCTATTGGTTTAACACCAGCATTGCTTGTTGTTTGCCGCGGT20210.10328841488313996No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC20140.1029306618380227No Hit
CCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAAC20050.10247069363715766No Hit
GCTCACACATTTTCTCATTTTTCTCTCAGCCAGAATCAGGCCCTTCTTTT19720.10078414356731916No Hit
GAGCGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACA19690.10063082083369747No Hit
AAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAACT19620.10027306778858022No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGCG100.0071125617144.85548
CATCGCC3100.0105.32002145
GGCACCC1200.0102.78454145
GTAACAA33500.087.336714
TGACGCC1300.083.71591145
ATAAGCC1500.082.22763145
AACAACG13300.080.051677
ACTCGCC755.4569682E-1177.39072145
ACAACGC13750.076.9050458
CAACGCA13750.076.9050459
GACGCGG300.001930331872.55379145
TAACAAG40450.071.614635
TAGTAAC41950.070.778452
GAACGCC851.6916601E-1068.28592145
CGCACCC1300.066.97273145
CGGTGCA1200.066.383572
GCCGACC1100.065.95799145
TTCGGAA554.045205E-665.95799145
GCTACCC451.1897675E-464.492256145
AGTAACA46400.064.148143
Produced by FastQC (version 0.11.5)