FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_709_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_709_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1759269
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA39560.22486612337283268No Hit
ACATATATGAATCCCTGATCTGTGGTTTTCTATATTTTCTCATAAATTCC39170.2226492935418063No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT35700.20292519222472513No Hit
GTATAAAAGCTATGTGATTAATTTGATAAGGGAATACAAGTACCACTCAA33430.19002210577234066No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT32570.18513371178597474No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT30320.17234430891466854No Hit
GCCATATACCGATGGGGTTATCCATGCAACTATCTCCATTCCTACAATGT29170.16580750300266758No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT27910.1586454373947361No Hit
GCTTTATGGTATGTGTCATGAATACAAATGACAGCCAATTTGGCACCTTA27310.1552349299623878No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAGTTACAGAAAACC26340.14972127628009133No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG24900.14153605844245534No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC24300.13812555101010704No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC23840.13551082864530667No Hit
ATATTACTCTTAGTGCTTTCCCAAAGGATCCTTTTACTAGTTTCCCTTCT23740.13494241073991525No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT23210.13192979584134093No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA22980.13062243465894072No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG22560.12823507945629692No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT21400.12164143175375682No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT20650.11737829746332142No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC20570.1169235631390083No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAA20360.11572988553768639No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA19020.10811308560544182No Hit
GAGTATGGATGGTTCTTCTTTTACCCAATCTGCTTATAGCTTTTTTTGTA18490.10510047070686745No Hit
GTCATAATGGATACCTTTATTACAAGAAACTTCCAGACTACAATAATACA18430.10475941996363261No Hit
TTGTAGAACCATGCTGCAAAGCCATATACCGATGGGGTTATCCATGCAAC18210.10350890057177156No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT17820.10129207074074517No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA17700.1006099692542755No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGTCTAAAGTCAATTCC17680.10049628567319721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCC202.1217875E-6145.12712145
GCCGGCC203.8530942E-4108.84534145
ACACGAG4550.093.9329157
GAAACAC6700.092.961274
AACACGA4750.091.50296
TCTCGGG406.0875755E-790.704445145
AAACACG6800.088.4116065
TGACGCC750.087.07627145
CACGAGC4950.086.342388
GCACGCA3700.084.1652452
AGCACGC3700.084.1652451
GCCGACC702.910383E-1182.92979145
GCAGCCC451.2268665E-680.62618145
ACGAGCC3950.078.860749
TAGAAAC8350.078.058842
CACGCAC4050.076.8917163
ACACGTG2100.075.8893057
AACACGT2100.075.8893056
ACAACGC2300.075.589358
CAACGCA2350.073.983169
Produced by FastQC (version 0.11.5)