FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_709_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_709_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1768890
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA39720.2245475976459814No Hit
ACATATATGAATCCCTGATCTGTGGTTTTCTATATTTTCTCATAAATTCC39290.22211669465031741No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT35820.20249987280158743No Hit
GTATAAAAGCTATGTGATTAATTTGATAAGGGAATACAAGTACCACTCAA33530.18955390103398176No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT32710.1849182255538784No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT30510.17248104743652798No Hit
GCCATATACCGATGGGGTTATCCATGCAACTATCTCCATTCCTACAATGT29300.16564059947198526No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT28030.1584609557406057No Hit
GCTTTATGGTATGTGTCATGAATACAAATGACAGCCAATTTGGCACCTTA27350.1546167370497883No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAGTTACAGAAAACC26430.14941573529162355No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG24970.14116197163192737No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC24430.1381092097303959No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC23920.13522604571228283No Hit
ATATTACTCTTAGTGCTTTCCCAAAGGATCCTTTTACTAGTTTCCCTTCT23840.13477378468983373No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT23290.13166449016049614No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA23070.1304207723487611No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG22620.12787680409748486No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT21490.12148861715539125No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT20710.11707907218651244No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC20700.11702253955870631No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAA20440.11555269123574671No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA19080.10786425385411189No Hit
GAGTATGGATGGTTCTTCTTTTACCCAATCTGCTTATAGCTTTTTTTGTA18540.10481149195258044No Hit
GTCATAATGGATACCTTTATTACAAGAAACTTCCAGACTACAATAATACA18520.10469842669696816No Hit
TTGTAGAACCATGCTGCAAAGCCATATACCGATGGGGTTATCCATGCAAC18290.10339817625742698No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT17900.10119340377298758No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA17760.10040194698370164No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGTCTAAAGTCAATTCC17710.10011928384467096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGCC301.0968506E-7120.929474145
ACGTGCC1750.0107.623229
GAAACAC6700.0104.8531954
AAACACG6750.0104.07655
ACACGTG2400.0102.61867
AACGGCC301.5934944E-596.74358145
AACACGT2550.096.582216
CACGTGC2500.095.6190958
GCACGCA3500.095.1836242
TAGAAAC8050.093.564252
AGCACGC3600.092.5396351
ACACGAG4950.092.192117
AACACGA4950.092.192116
GCAGCCC406.090522E-790.69711145
CACGCAC3750.088.838053
CACGAGC5450.083.736488
GAGCTCC702.910383E-1182.92307145
CGCACCC353.424471E-582.92307145
ACGTGCG353.452462E-582.787099
GCTGCCC1550.079.5794145
Produced by FastQC (version 0.11.5)