FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_703_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_703_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1374600
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT99470.7236286919831224No Hit
GTTGTGTCCTAGTGACCCTAGGTTTAATGACACACACACACACACACACA60400.4394005528881129No Hit
GTCCTAGTGACCCTAGGTTTAATGACACACACACACACACACACACACAC50910.3703622872108249No Hit
CACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGACACTGTTGTG45070.3278772006401862No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGAC35640.2592754255783501No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC34430.2504728648334061No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGG34020.2474901789611523No Hit
CACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGACACTGTTGTGTCCT33150.2411610650371017No Hit
CTTTTAAGGCCGTGTTTGAATAGTCGATAGACGCATTTGAAAAAAAGACG31510.2292303215480867No Hit
GTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACACAACAGCGTCC30220.21984577331587374No Hit
GTGTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACACAACA29990.21817255928997525No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTA28750.2091517532373054No Hit
GTGTGTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACACAA28470.2071147970318638No Hit
GTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACACAACAGCGT28290.2058053251855085No Hit
GTGTGTGTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACAC27680.2013676705950822No Hit
GTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACACAACAGC26170.19038265677287938No Hit
CTGTTGTGTCCTAGTGACCCTAGGTTTAATGACACACACACACACACACA25870.18820020369562054No Hit
CACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACGCTGTTCTGTCCT23950.17423250400116397No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC23700.1724137931034483No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG22040.16033755274261602No Hit
CACACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGACACTGTTG21830.15880983558853484No Hit
CACACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACGCTGTTCTG21270.15473592317765167No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTT19640.1428779281245453No Hit
CACACACACACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACGCT19620.14273243125272808No Hit
CACACACACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACGCTGT19600.1425869343809108No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT19500.14185945002182454No Hit
CACACACACACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGACA18880.1373490469954896No Hit
GGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAG18860.13720355012367233No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC18550.13494834861050486No Hit
CACACACACACACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACG18410.13392987050778407No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC18200.1324021533537029No Hit
CACACACACACACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGA17870.13000145496871818No Hit
CACACACACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGACACT17850.12985595809690093No Hit
CACACACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGACACTGT17640.12832824094281972No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA17520.1274552597119162No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA17450.1269460206605558No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC17320.12600029099374363No Hit
TCATTAAACCTAGGGTCACTAGGACACAACAGCGTCCTGCTACAGTTCCC17300.12585479412192638No Hit
GTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACACAACAGCGTCCTG17020.12381783791648479No Hit
GTGTCCTAGTGACCCTAGGTTTAATGACACACACACACACACACACACAC16380.11916193801833261No Hit
GCTATAACTATTTTAGTGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA16170.11763422086425142No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT16170.11763422086425142No Hit
CACACACACACACACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGA16030.11661574276153064No Hit
CACACACACACACACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAG15780.11479703186381492No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT15710.11428779281245453No Hit
AACCTAGGGTCACTAGGACACAACAGCGTCCTGCTACAGTTCCCCACACC15660.1139240506329114No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA15460.11246908191473884No Hit
GTTCTGTCCTAGTGACCCTAGGTTTAATGACACACACACACACACACACA15340.1115961006838353No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC15180.11043212570929725No Hit
ACCTAGGGTCACTAGGACACAACAGCGTCCTGCTACAGTTCCCCACACCA14850.10803142732431253No Hit
CACACACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACGCTGTTC14790.10759493670886076No Hit
ACGCTGTTGTGTCCTAGTGACCCTAGGTTTAATGACACACACACACACAC14620.10635821329841408No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG14530.10570347737523644No Hit
CACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACGCTGTTCTGTC14470.10526698675978466No Hit
CTGTAGCAGGACACTGTTGTGTCCTAGTGACCCTAGGTTTAATGACACAC14300.10403026334933799No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG14150.10293903681070857No Hit
CACACACACACACACACACACACACCTGCTGGTGTGGGGAACTGTAGCAG13840.1006838352975411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC8350.079.802543
GCGAAAG9750.067.595932
ATAAGCC451.18966294E-464.49203145
GGGTGAC4700.063.176271
CAGTACG3500.062.0959639
CGAATAA1500.058.042828145
AAGCTAA1550.051.4034355
CAAGCTA1600.049.795274
TAAACTA4400.049.3855365
AGCGAAA13400.049.1818161
TACAATC901.3311801E-648.2951057
GTACTGA2600.047.3663566
TGACGCC2200.046.17043145
ACAAGGT5350.044.6858838
TTAGGTG855.3721495E-542.607135
ATATTCA3150.041.3882754
AGTAGAA9700.040.3171651
TATATTC3250.040.114793
TACATTG3100.039.7266166
CGTACTC3350.038.917333
Produced by FastQC (version 0.11.5)