FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_703_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_703_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1381741
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT99730.7217705778434599No Hit
GTTGTGTCCTAGTGACCCTAGGTTTAATGACACACACACACACACACACA60580.43843238349299907No Hit
GTCCTAGTGACCCTAGGTTTAATGACACACACACACACACACACACACAC51070.3696061707657224No Hit
CACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGACACTGTTGTG45170.3269064173387053No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGAC35750.2587315567823492No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC34480.24954025392602522No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGG34120.24693484524234283No Hit
CACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGACACTGTTGTGTCCT33250.2406384409234437No Hit
CTTTTAAGGCCGTGTTTGAATAGTCGATAGACGCATTTGAAAAAAAGACG31640.2289864743103085No Hit
GTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACACAACAGCGTCC30270.21907144681962828No Hit
GTGTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACACAACA30070.21762399755091583No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTA28880.2090116744020768No Hit
GTGTGTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACACAA28490.20618914832808755No Hit
GTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACACAACAGCGT28410.20561016862060255No Hit
GTGTGTGTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACAC27730.20068884110698024No Hit
GTGTGTGTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACACAACAGC26250.18997771651850817No Hit
CTGTTGTGTCCTAGTGACCCTAGGTTTAATGACACACACACACACACACA25910.187517052761697No Hit
CACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACGCTGTTCTGTCCT24040.17398340209923568No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC23740.171812228196167No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG22110.1600155166561606No Hit
CACACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGACACTGTTG21900.15849569492401253No Hit
CACACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACGCTGTTCTG21290.15408097465443957No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTT19710.1426461254316113No Hit
CACACACACACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACGCT19710.1426461254316113No Hit
CACACACACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACGCTGT19680.14242900804130443No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT19560.14156053848007694No Hit
CACACACACACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGACA18920.13692870082019712No Hit
GGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAG18880.13663921096645465No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC18570.13439566459995037No Hit
CACACACACACACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACG18440.13345482257528726No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC18230.1319350008431392No Hit
CACACACACACACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGA17940.12983619940350616No Hit
CACACACACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGACACT17900.12954670954976366No Hit
CACACACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAGGACACTGT17640.12766502550043748No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA17580.12723079071982377No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA17480.12650706608546752No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC17380.12578334145111134No Hit
TCATTAAACCTAGGGTCACTAGGACACAACAGCGTCCTGCTACAGTTCCC17360.1256385965242401No Hit
GTGTGTGTGTGTCATTAAACCTAGGGTCACTAGGACACAACAGCGTCCTG17080.12361216754804266No Hit
GTGTCCTAGTGACCCTAGGTTTAATGACACACACACACACACACACACAC16440.11898032988816283No Hit
GCTATAACTATTTTAGTGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA16250.11760525308288601No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT16190.11717101830227226No Hit
CACACACACACACACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGA16100.11651966613135169No Hit
CACACACACACACACACACACAGACCTGCTGGTGTGGGGAACTGTAGCAG15850.11471035454546112No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT15780.11420374730141178No Hit
AACCTAGGGTCACTAGGACACAACAGCGTCCTGCTACAGTTCCCCACACC15720.11376951252079805No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA15530.11239443571552121No Hit
GTTCTGTCCTAGTGACCCTAGGTTTAATGACACACACACACACACACACA15380.11130884876398688No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC15270.11051275166619505No Hit
ACCTAGGGTCACTAGGACACAACAGCGTCCTGCTACAGTTCCCCACACCA14900.10783497051907703No Hit
CACACACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACGCTGTTC14820.10725599081159204No Hit
ACGCTGTTGTGTCCTAGTGACCCTAGGTTTAATGACACACACACACACAC14640.10595328646975084No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG14560.10537430676226586No Hit
CACACACACACCTGCTGGTGTGGGGAACTGTAGCAGGACGCTGTTCTGTC14540.10522956183539461No Hit
CTGTAGCAGGACACTGTTGTGTCCTAGTGACCCTAGGTTTAATGACACAC14330.10370974010324656No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG14160.10247940822484097No Hit
CACACACACACACACACACACACACCTGCTGGTGTGGGGAACTGTAGCAG13890.10052535171207917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC9450.087.376043
GCGAAAG11450.072.108632
CGAATAA1154.0017767E-1156.77075145
GGGTGAC5100.055.3837431
GTACTGA1600.054.342276
TATATTC3150.052.8854943
AGCGAAA15700.052.588781
ATATTCA3450.050.386184
TGACGCC2600.050.220272145
TTATGGG604.9693097E-448.286763
TAGAAAC9900.048.283262
GTATTAA5900.046.646541
TACATTG2500.046.372076
CAGTACG3050.045.1367459
GCAGGGT3400.044.7524578
GGTCGTT1755.2750693E-1141.4036337
CAAGGGT11100.038.49894
TAAACTA4550.038.206295
CGGTCTA17300.038.157085145
TCTATGC7050.036.98563
Produced by FastQC (version 0.11.5)