FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_463_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_463_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1707309
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG68770.40279761894302674No Hit
ATTATAGATACTCTAACGCCAAGGTCAGCTGCTGGTACAAGTACCTCCTT47880.2804413260868419No Hit
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC39880.2335839616613044No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT38710.22673107211406957No Hit
GTGTGGATATGATGTCAGGATTCAGCTGAAGAGCAAGTGGGATGAGTCCA37790.22134247520513278No Hit
TATCCACACTGTGTGTTCACAACTTTTTGCGCAGGATCTGGCACGCAGCA37720.2209324732664093No Hit
CTTATGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAG29510.17284510302470146No Hit
CTATTATAGATACTCTAACGCCAAGGTCAGCTGCTGGTACAAGTACCTCC28320.16587507006640276No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT28180.16505506618895582No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA27720.16236076773448743No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT24680.14455496925278316No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA23020.13483206613448415No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAATC22810.1336020603183138No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA22130.12961918434214312No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG21490.1258705951881001No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATGGATAAG21430.12551916495490856No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC21190.12411344402214244No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCCCGTTGGGAAACAAACTGTG21180.12405487231661053No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC20980.1228834382059721No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT20330.11907627734639717No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGGCCACTT20070.1175534130025672No Hit
AGCAGGGACCGCCCACAGTGTTTCTACCAATTTTCAAAACCGTTTGATTC20040.11737769788597142No Hit
ATCTAAGGCTGCTCAGAGGAGAATGCTGGAGGAGAGGAAGAGGAAGTGAA19220.11257481803235383No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCCTGATG18810.11017337810554505No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCC18630.10911908740597046No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGTTCCCGCCACTTCTCATTTC17880.1047262094910763No Hit
GTCCTACATTGTGGAAACATCTAATTCAGACAATGGAACGTGTTACCCAG17800.10425763584682092No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTA17560.1028519149140548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATTCC1950.0100.44472145
CGAAAGC12050.089.572243
GTAGAAA8550.088.110721
CGCAGAA259.3655963E-487.05209145
GCACGGG1250.081.24862145
CCGCACC651.5570549E-978.12367145
GTCGGCC950.076.36149145
GCGAAAG15400.069.614952
CGAGTCC651.392873E-766.96315145
CTGGACC554.048423E-665.948555145
CCGCCCA5300.065.611179
GGACCGC5300.065.607326
TCTCGGG851.2998498E-859.74163145
ACCGCCC5950.058.4435658
CGACGAA753.7472637E-758.03473145
TAACTTT553.2101458E-452.758846145
TACTAAT1259.094947E-1152.157837
TCTACTA1259.094947E-1152.1562965
TCTGACC1405.456968E-1251.81672145
GAAAGCA21100.051.153824
Produced by FastQC (version 0.11.5)