FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_463_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_463_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1715853
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG68960.40189923029536917No Hit
ATTATAGATACTCTAACGCCAAGGTCAGCTGCTGGTACAAGTACCTCCTT48080.28021048423145806No Hit
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC39970.23294536303517843No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT38850.2264179973459265No Hit
GTGTGGATATGATGTCAGGATTCAGCTGAAGAGCAAGTGGGATGAGTCCA37910.22093967257101862No Hit
TATCCACACTGTGTGTTCACAACTTTTTGCGCAGGATCTGGCACGCAGCA37880.22076483241862793No Hit
CTTATGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAG29560.17227583015561354No Hit
CTATTATAGATACTCTAACGCCAAGGTCAGCTGCTGGTACAAGTACCTCC28440.16574846446636163No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT28230.1645245833996269No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA27820.1621351013169543No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT24760.14430140577310527No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA23080.13451035723922736No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAATC22880.1333447562232895No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA22190.12932343271830396No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG21590.1258266296704904No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATGGATAAG21480.1251855491117246No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC21280.12401994809578677No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCCCGTTGGGAAACAAACTGTG21250.1238451079433961No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC20980.12227154657188No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT20380.11877474352406646No Hit
AGCAGGGACCGCCCACAGTGTTTCTACCAATTTTCAAAACCGTTTGATTC20140.11737602230494104No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGGCCACTT20130.11731774225414415No Hit
ATCTAAGGCTGCTCAGAGGAGAATGCTGGAGGAGAGGAAGAGGAAGTGAA19280.1123639379364083No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCCTGATG18870.10997445585373572No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCC18650.10869229473620409No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGTTCCCGCCACTTCTCATTTC17930.10449613107882784No Hit
GTCCTACATTGTGGAAACATCTAATTCAGACAATGGAACGTGTTACCCAG17830.10391333057085893No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTA17600.1025728894025304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC11500.088.177823
AGATTCC1500.087.06275145
GTAGAAA9800.084.996481
GTTTCGC300.001930449372.55229145
TCGATAA300.001930449372.55229145
CTCGAAA300.001930449372.55229145
GACGCTC300.001930449372.55229145
GGACCGC4150.068.0723956
GCGAAAG15800.063.721632
GTTACGC4050.062.5953673
CGCAACC350.003556764262.18768145
CATTACG350.00358026562.0843851
CCGCACC1200.060.460243145
ACGGGCC851.2987584E-859.748947145
GACCTCC1251.8189894E-1258.041836145
GTCGGCC753.7440986E-758.041836145
CCGCCCA4950.057.0774469
TCTCGGG902.0390871E-856.429558145
ATTACGA400.006074199554.3238332
TACGAGC400.006074199554.3238334
Produced by FastQC (version 0.11.5)