FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_455_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_455_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391632
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG64330.4622630120606597No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT42170.3030255124918082No Hit
TTATAAAAGCTTGTGTTGTAGTATGGTTAAAGAAACAGCACAGAACTTAC37420.2688929257159939No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA36470.26206640836083106No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT32950.23677236510801705No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT32880.23626935856605769No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT32790.23562263586925278No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAA32670.23476033894017956No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT29890.21478379341665038No Hit
TGTAAGCAGAGTGCAGAAAACATTTTTGTAATGTGTGTGTTTGACTGGGT27380.19674741598353587No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC26870.19308265403497477No Hit
GACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAA26590.1910706278671373No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC26550.19078319555744622No Hit
ACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAAT24080.1730342504340228No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA23710.17037550156938042No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC23710.17037550156938042No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG23160.16642330731112823No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC22910.16462685537555907No Hit
CAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATT21800.15665060878163192No Hit
GAGCGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACA21390.15370442760729847No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCAGCAGAAAGCCCCT21380.15363256952987572No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA19690.1414885544454281No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG18530.13315301746438715No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA18380.13207514630304565No Hit
CTCAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTT17570.1262546420318015No Hit
GTATAGCACCTGTCTTGTTCTCCAACAAAATAGCCAGATTAGGGAAAGGA17450.12539234510272831No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGAATCTTTTTGGCTATGACT17350.12467376432850064No Hit
GCGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACATG17170.12338031893489085No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT17160.12330846085746806No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT16940.1217275831541672No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT16730.1202185635282891No Hit
CCATAAAGTGATGGAGTTTGAACGATGGAGGTTTAAGGCATTTTGTAAGA16480.11842211159271991No Hit
CTTCTGTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATC16460.11827839543787437No Hit
GTAGTAACAAGAGCATTTTTCAGAACCAATCAAGTTCAGTAAGGACAATT16450.11820653736045161No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT16420.1179909631281833No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT16250.11676937581199627No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT15990.11490106579900433No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC15860.11396691079250837No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA15840.11382319463766283No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATATATCACCCGGG15660.11252974924405304No Hit
GATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTTACTTGTTATC15310.11001471653425618No Hit
GCACAGAGCGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG15240.10951170999229681No Hit
GTCTATAGCTGTCAGACATGATTTTAAAGGTTCAGTATCTATCACGGTCT15130.10872127114064639No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGCTACGCTA15050.10814640652126424No Hit
TTGTAAGAGTCTGTCAACTCCACAACTCCACTTTTTTACTCCGACAACAC15020.10793083228899596No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT14980.10764339997930489No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT14930.10728410959219103No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGC14920.10721225151476828No Hit
ACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATTCAGGCT14770.10613438035342677No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT14750.10599066419858123No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA14650.10527208342435357No Hit
ATATGGAACTTCTGGTAGCTCATGGTTGTAAATGTGAGGCCCTATTGGTC14460.10390677995332098No Hit
AAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATTCAGG14000.10060130839187373No Hit
CAATACTTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGCCT13990.10052945031445094No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA13980.10045759223702819No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTCA100.007075022145.11052145
AGCACGC2150.0117.9181
GCTGCCC1300.0106.04231145
GCGAGAA301.5936386E-596.74035145
GCGGTGC1050.089.681851
GCCGACC900.088.67866145
CATCGGG259.3591516E-487.066315145
GGTGCAT502.2828317E-886.925533
CGATAAA608.258212E-1084.647804145
GCACGCA3050.080.74762
GCAGCCC451.227565E-680.61696145
CGGTGCA554.4230546E-879.020382
ACGTGCA755.638867E-1177.2754759
AACACGT3450.075.595576
ACACGTG3500.074.515637
TGACGCC2000.072.55526145
CAAGCCC406.638991E-572.55526145
CGCACTG300.001942212772.440555
TAGTAAC14150.070.6436542
GTAACAA14900.066.1202244
Produced by FastQC (version 0.11.5)