FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_455_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_455_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1403652
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG64750.4612966746743495No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT42460.3024966302188862No Hit
TTATAAAAGCTTGTGTTGTAGTATGGTTAAAGAAACAGCACAGAACTTAC37780.26915503272891006No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA36710.2615320606532104No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT33170.2363121343466899No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT33050.2354572215905367No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT33020.2352434934014984No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAA32870.23417485245630681No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT30080.21429813087574415No Hit
TGTAAGCAGAGTGCAGAAAACATTTTTGTAATGTGTGTGTTTGACTGGGT27540.19620247753716732No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC27090.1929965547015927No Hit
GACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAA26740.19050305916281243No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC26720.19036057370345358No Hit
ACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAAT24270.17290610493199168No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA23880.17012763847449366No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC23850.16991391028545538No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG23360.16642301653116298No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC23100.16457070555949765No Hit
CAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATT21920.15616406345732417No Hit
GAGCGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACA21520.15331435427014672No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCAGCAGAAAGCCCCT21480.153029383351429No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA19800.1410606047652837No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG18730.13343763268958403No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA18410.13115786533984208No Hit
CTCAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTT17700.12609963153260212No Hit
GTATAGCACCTGTCTTGTTCTCCAACAAAATAGCCAGATTAGGGAAAGGA17600.12538720423580774No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGAATCTTTTTGGCTATGACT17510.12474601966869282No Hit
GCGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACATG17330.12346365053446295No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT17240.12282246596734803No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT17050.12146885410343874No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT16830.11990151405049114No Hit
CCATAAAGTGATGGAGTTTGAACGATGGAGGTTTAAGGCATTTTGTAAGA16590.11819168853818467No Hit
CTTCTGTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATC16570.1180492030788258No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT16570.1180492030788258No Hit
GTAGTAACAAGAGCATTTTTCAGAACCAATCAAGTTCAGTAAGGACAATT16560.11797796034914637No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT16360.11655310575555765No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT16110.11477203751357175No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC15940.11356091110902133No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA15870.11306221200126527No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATATATCACCCGGG15750.11220729924511204No Hit
GATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTTACTTGTTATC15390.10964256097665233No Hit
GCACAGAGCGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG15320.10914386186889628No Hit
GTCTATAGCTGTCAGACATGATTTTAAAGGTTCAGTATCTATCACGGTCT15200.10828894911274305No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGCTACGCTA15150.10793273546434587No Hit
TTGTAAGAGTCTGTCAACTCCACAACTCCACTTTTTTACTCCGACAACAC15120.10771900727530755No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT15080.10743403635658981No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGC15040.10714906543787207No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT14990.1067928517894749No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT14850.10579545357396278No Hit
ACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATTCAGGCT14850.10579545357396278No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA14740.10501178354748897No Hit
ATATGGAACTTCTGGTAGCTCATGGTTGTAAATGTGAGGCCCTATTGGTC14520.10344444349454139No Hit
AAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATTCAGG14090.10038100611832564No Hit
CAATACTTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGCCT14080.10030976338864618No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA14050.10009603519960787No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGTC100.0070741987145.11623145
TGCAGCC151.2261904E-4145.11623145
CCCGCCC100.0070741987145.11623145
GCTGCCC1750.0111.946785145
AGCACGC1950.0111.440961
GCCGACC800.0108.837166145
CGTGCTC259.35771E-487.06973145
TCGTCCC259.35771E-487.06973145
CTGGCTT259.35771E-487.06973145
GCACGCA2600.080.794712
TAGTAAC13300.079.51692
CGATAAA554.3713953E-879.154305145
CGCACTG554.420326E-879.027415
GTAACAA13850.075.841584
GCAGCCC300.00192976272.55811145
TGACGCC2100.072.558105145
GAGCGGT300.001941534872.446956
GTAGTAA15800.068.768961
GAACGCC754.825779E-967.7209145
GCGGAGC1450.064.9431841
Produced by FastQC (version 0.11.5)