FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_420_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_420_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272095
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT11940.4388173248314008No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT7370.27086128006762344No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA7070.2598357191422114No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA6580.2418273029640383No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT6120.22492144287840646No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT5490.20176776493504106No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT5430.19956265274995866No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA4570.16795604476377735No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT4410.16207574560355759No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTTTGTAATGATGACAAGCAA4400.16170822690604383No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG4390.1613407082085301No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC4290.15766552123339275No Hit
CTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC4200.15435785295576912No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA4130.15178522207317297No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG4090.15031514728311804No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTTTGTAATGATGACAAGCAA3970.1459049229129532No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT3920.14406732942538453No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG3920.14406732942538453No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT3810.14002462375273342No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG3660.13451184329002738No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT3630.13340928719748618No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA3580.1315716937099175No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC3300.1212811701795329No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT3270.12017861408699167No Hit
CCATTAGAGAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA3240.11907605799445047No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA3150.11576838971682683No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT2960.10878553446406587No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG2900.10658042227898344No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA2880.10584538488395596No Hit
GTATTAAGAGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA2750.1010676418162774No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA2740.10070012311876367No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC2730.10033260442124993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCCC202.1215947E-6145.08488145
CGTGCAG100.0071146763144.818243
GCACGCA1300.0139.24832
CACGCAC1400.0134.474063
AGCACGC1350.0134.090961
CGCACTT1600.0117.664815
GGTGCGT256.478942E-6115.91856
AACACGT256.478942E-6115.91856
AAGCGGT256.496737E-6115.854592
CGGTGCG256.496737E-6115.854595
ACGCACT1650.0114.099224
ACGTGCA458.54925E-11112.6364062
AAACACG700.0103.441595
GAAACAC750.096.5454944
AGCGGTG301.6073373E-596.545493
CACGTGC850.093.705911
CAAGAGG1400.087.9738548
GTGCGTT353.4447563E-582.798937
CGTGCCT353.4541954E-582.753273
ACGTGCC353.4541954E-582.753272
Produced by FastQC (version 0.11.5)