FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_412_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_412_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1888473
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC119800.6343749685592539No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT86230.4566122999905215No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT71760.3799895471102844No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAG67990.36002632814978025No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC59020.3125276347609947No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT57700.3055378604830464No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCGTCGGAGGACTTGAG57230.3030490772174132No Hit
GAGGAGAGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG53050.28091479200391006No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTG51230.2712773759540115No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT50350.2666175264353793No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA46580.2466543074748752No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC46170.2444832412218761No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC43780.2318275135519544No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTA43310.2293387302863213No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC41020.2172125309707896No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA40840.21625937993288757No Hit
GTGTTACCATTCCACTCAAGTCCTCCGACGAGGACCCCAACTGCATTTTT39150.2073103507436961No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT38680.20482156747806296No Hit
CTTTCAATCTGTGCCGTATTTCTTCAATTAACCACCTTATTTCCACAAAT38030.20137963317452778No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG37630.19926151975696768No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT36520.19338375502323835No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA36280.1921128869727023No Hit
ACATAATGGAATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTT35370.18729417894775302No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT34180.18099279153051168No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC34160.18088688585963367No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC32620.17273214920202723No Hit
ACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCC32470.1719378566704422No Hit
GGTTCATGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA32220.17061403578446713No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAGCT32180.17040222444271114No Hit
GATAAGAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATT32020.16955497907568706No Hit
GTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCCAGT31290.16568942208863988No Hit
ACTAAGGGCTTTCACTGAGGAGAGAGCAATAGTTGGAGAAATTTCACCAT31180.16510694089881084No Hit
CTACTAAGGGCTTTCACTGAGGAGAGAGCAATAGTTGGAGAAATTTCACC31080.1645774125444208No Hit
GTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGATCCAGC31060.1644715068735428No Hit
TCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG30900.16362426150651876No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG30490.16145319525351964No Hit
GTACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGG30420.16108252540544662No Hit
TCTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCT30190.15986461019034956No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA30150.15965279884859354No Hit
GCTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA30070.1592291761650815No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGTATTTCTTCAATTAACCA30060.1591762233296425No Hit
ATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTATCTTTT29950.15859374213981348No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG29950.15859374213981348No Hit
CCTTATTTCCACAAATTTCTGTCCCAATTGTTCCCGCCACTTCTCATTTC29910.15838193079805749No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC29220.1547281851527663No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGAGAGCAATAGTTGGAGAA29140.15430456246925425No Hit
GTGCCGTATTTCTTCAATTAACCACCTTATTTCCACAAATTTCTGTCCCA29140.15430456246925425No Hit
GGCATAAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGA28740.15218644905169415No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT28660.1517628263681821No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC28370.15022719414045105No Hit
CTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT28110.14885042041903698No Hit
GCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGAT27820.1473147881913059No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCTTCTCCAAGCG27110.14355513687513669No Hit
CTCTTGTTGGGAAACAAATTGTGGAATGGATTTTGAAAGAGGAATCCAGC26810.1419665518119666No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC26680.14127816495125958No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACG26610.14090749510318654No Hit
GCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG26350.13953072138177247No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAA26340.13947776854633345No Hit
GCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAATGTAA26220.13884233452106542No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCTATGAC25860.1369360324452613No Hit
CTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTGAAGA25860.1369360324452613No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT25670.1359299285719203No Hit
CCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGC25610.13561221155928624No Hit
GTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACCTTGAT25470.1348708718631402No Hit
GAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGA25450.13476496619226222No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC25190.13338819247084813No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC25130.13307047545821413No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC25070.1327527584455801No Hit
TTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTTATG25040.1325938999392631No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT25010.1324350414329461No Hit
GTACCTACTTCGCGTTACATTTCTGACATGACCCTCGAGGAAATGTCACG24810.13137598472416603No Hit
CATCTGTACCTACTTCGCGTTACATTTCTGACATGACCCTCGAGGAAATG24710.130846456369776No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTT24700.130793503534337No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAG24620.130369880850825No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATGGATAAG24220.12825176743326486No Hit
GTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCT24190.12809290892694786No Hit
CTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT24130.12777519191431386No Hit
GCATGTACCATCTGCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGC24060.1274045220662408No Hit
GTCTTATACAATCCAGTCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG23820.12613365401570475No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG23460.12422735193990066No Hit
GTTCACAAGTGGCACAAACTAGACCAAAAGCAGCTTCTGTGGTCACTGTT23440.12412144626902265No Hit
GTCATGGATAAGAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAA23080.12221514419321854No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATA23060.12210923852234053No Hit
GTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATAAGCA22960.12157971016795051No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCT22610.1197263609275854No Hit
CTCCCATTCTCATCACAGCTTCTCCAAGCGAATCTCTGTATATTTTCAGA22430.11877320988968336No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAGC22400.11861435138336635No Hit
ACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACC22220.11766120034546429No Hit
GCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCC21950.11623147378861122No Hit
GTTTTCTTTGGCATATTCGCAAGCGATTTGCAGACAATGGATTGGGTGAT21900.11596670961141621No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTTCCATTTAGGGCATTTTGGAT21680.11480174723175814No Hit
CTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGT21680.11480174723175814No Hit
GTTGAAGATTACACTGAAGTTTGCTTCCAGTACTATGTTCTTATCCATGA21640.11458993589000213No Hit
GGGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGC21640.11458993589000213No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT21540.1140604075356121No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA21520.1139545018647341No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTGGAA21300.11278953948507604No Hit
GCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGAT20990.11114800158646695No Hit
GTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAA20640.10929465234610185No Hit
ATCTTCAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA20580.10897693533346783No Hit
GCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGACC20400.10802378429556579No Hit
CTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACT20360.10781197295380977No Hit
TGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGAC20100.1064351992323957No Hit
GTTCATGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGAT20070.1062763407260787No Hit
CTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACC20000.10590567087800566No Hit
TGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA19910.10542909535905465No Hit
GCTATGACGAGAGGATCACTTGAACCGCTGCATCTGCACTCCCATTCGCT19900.10537614252361564No Hit
ATTCTCTGTCGCTTTCAATCTGTGCCGTATTTCTTCAATTAACCACCTTA19870.10521728401729864No Hit
ATCCATGTTGTTCGGGTCCCCATTTCCATTTAGGGCATTTTGGATAAAGC19770.1046877556629086No Hit
AAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAGCTTTC19750.10458184999203059No Hit
CCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCTATGACGAGAG19630.10394641596676256No Hit
GTAGTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCC19620.10389346313132357No Hit
CTATTGCTCTCTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAAT19580.10368165178956754No Hit
CTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGT19390.1026755479162265No Hit
AGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAATGTA19350.10246373657447048No Hit
CATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTGGAATGGATTTTGA19330.10235783090359248No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGTA100.0071031647144.919339
CCTCGCC1300.0122.750824145
TCACGAA301.09896064E-7120.890976145
ACCTCCC700.0113.98291145
AGGCTCC2200.0108.80187145
CGTCGGG404.802132E-9108.801865145
GAGCGAA1650.0100.985671
AATCGCC700.093.25874145
TGACGCC900.088.65338145
GCGAAAG6650.088.247462
CGAAAGC6700.087.593543
CATCTCG259.415754E-486.9354861
AGTAGAA6950.086.518531
TACCGAT608.3491614E-1084.529575
CGATTAA353.429946E-582.89666145
TCACGCC1850.082.336555145
ACTGACC801.8189894E-1281.6014145
ATACCGA651.5734258E-978.027294
TTCTACT1850.074.4121254
CGACGGG300.001932350172.534584145
Produced by FastQC (version 0.11.5)