FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_411_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_411_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1738607
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT55860.32129170076963914No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG50950.2930507009347138No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT44850.25796514105833007No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAA44620.2566422428990565No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT42330.24347077861759442No Hit
ACCTAAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAACGCTCTGT41530.2388693937157736No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA35590.20470411081975398No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC35170.20228838374629804No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA34680.19947003549393277No Hit
CTACTATATTGTCTCATGGTCATGTACCTAAATCCTGGAAATTATTCAAT33680.19371830436665677No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA31530.1813520824430133No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT28060.16139357543136545No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT27660.15909288298045504No Hit
ACTATATTGTCTCATGGTCATGTACCTAAATCCTGGAAATTATTCAATGC27320.1571372943971812No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA26910.154779084634998No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT26580.15288101336299692No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG25270.14534624558626533No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC25150.14465603785099218No Hit
ATATTGTCTCATGGTCATGTACCTAAATCCTGGAAATTATTCAATGCAAG24920.1433331396917187No Hit
ATAATAAACAGTGAAGACGAGCATCTATTGGCACTTGAGAGAAAACTAAA24880.14310307044662768No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT24160.13896182403498894No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA24080.13850168554480685No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA23690.13625851040516918No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG23450.13487809493462294No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT23080.13274995441753082No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG22920.13182967743716664No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA22690.13050677927789317No Hit
TCTCAGAGCTGACACCATAAGCTCACAAATAGAAATTGCAGTCTTGCTTT21990.12648056748879993No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT21320.122626907633525No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT20410.11739283230770381No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT19670.11313655127351956No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGC19170.11026068570988153No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT19140.11008813377606326No Hit
CCTAAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAACGCTCTGTG19100.10985806453097222No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT19080.10974302990842669No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT19000.1092828914182446No Hit
GATTATAGGTACACATACAAATGCCACAGAGGAGATTCCAAAGTGGAAGG18870.10853516637169872No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT18600.10698219896733419No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGA18470.1062344739207883No Hit
CCATATAAACAATAAAAATAGCTAGCATTAATGTTACAGCCAAACTAGAA18340.10548674887424242No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC18030.10370371222478685No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT18020.1036461949135141No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT17930.10312853911205926No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC17890.10289846986696821No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC17820.10249584868805889No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG17790.1023232967542406No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA17500.10065529472733055No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA17480.10054026010478505No Hit
ACCTAATGGACCCTGAATACAAAGGGCGGTTACTTCACCCTCAAAATCCC17410.10013763892587571No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACACC100.0071128144.853325
ATATGCC100.0071140234144.844973
ACGGGAG100.0071140234144.844974
AGCCGAC100.0071146353144.840822
AGCACGC5650.0124.3288041
GCACGCA5850.0121.319662
CACGCAC5950.0119.2840963
GGACGGG203.8560005E-4108.82465145
CACACCC600.0108.82465145
CGGTGCA950.0106.724812
GGCACCC1350.0102.10708145
GCGGTGC1800.088.5112841
GGTGCAT1150.088.166523
CGTGCTC259.362362E-487.05972145
GCCGACC259.362362E-487.05972145
GACGCTT259.362362E-487.05972145
TATGTCC259.362362E-487.05972145
CGTGCTG1550.084.103533
GTAACAA17600.083.532774
GAAACAC5550.083.514224
Produced by FastQC (version 0.11.5)