FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_274_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_274_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568765
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC49390.3148336430249273No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA43120.2748658976965957No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC36400.23202965389972366No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT34890.22240424792750987No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG30440.19403798529416452No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT30090.1918069309297441No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT28110.17918553766816572No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA25930.1652892561983471No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA24540.15642878315107744No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTT22810.14540100014979937No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA22290.14208629080837476No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT21950.13991898085436633No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT21000.1338632618652252No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA20880.13309832894028106No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAGCA19970.12729758759278795No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA18510.1179909036726342No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC18430.1174809483893381No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT18300.11665227105398196No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA17820.11359253935420538No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA17630.11238139555637715No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA17570.11199892909390508No Hit
TCATAATCCTCTGCTGTGTCCCTCCCAAAGAAGAAATTGGGGATGGTCTG17000.1083654977004204No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCC100.0070770094145.09749145
CACGTAA100.0070770094145.09749145
CACCGGC100.0070770094145.09749145
CTAGGTT151.2268484E-4145.09749145
TGACGCC403.274181E-11126.9603145
CGCACCC301.0975964E-7120.91457145
AGCACGC4650.0110.575621
GGTACCC203.8561475E-4108.82311145
GTAACAA17350.0107.693154
CACGCAC4850.0106.0158163
AAACACG4550.0101.874195
GCACGCA5050.0101.817172
GAAACAC4500.0101.390194
ACGAGCT301.6055683E-596.595959
ACGCACT5850.090.372214
CGCACTT5900.089.6120455
GCCGACC259.36272E-487.05848145
AACACGT1900.083.877936
CGTGCTC353.4264456E-582.91284145
CGTAAAA353.4264456E-582.91284145
Produced by FastQC (version 0.11.5)