FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_274_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_274_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580042
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC49570.3137258376676063No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA43400.2746762427834197No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC36560.23138625428944293No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT35030.22170296738947443No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG30570.19347586962878202No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT30190.19107087026800554No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT28300.17910916292098564No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA26120.1653120613249521No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA24720.15645153736419665No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTT22900.14493285621521454No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA22450.14208483065640026No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT22070.13967983129562378No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT21110.13360404343682003No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA20930.13246483321329433No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAGCA20090.12714851883684103No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA18620.1178449686780478No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC18550.11740194248001001No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT18420.11657917954079702No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA17920.11341470669767008No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA17640.11164260190551897No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA17630.11157931244865642No Hit
TCATAATCCTCTGCTGTGTCCCTCCCAAAGAAGAAATTGGGGATGGTCTG17080.10809839232121678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGCC202.12357E-6145.10168145
CTCGGCC256.4435444E-6116.08135145
TCTCGCC203.855708E-4108.82626145
GCACGCA4400.0108.6437452
AGCACGC4400.0108.6437451
GTAACAA18000.0107.8457954
CACGCAC4550.0105.0687263
CGGTGCA950.099.113592
CGCACCC301.5941876E-596.73447145
AAACACG5050.094.6658865
GAAACAC5300.090.2005164
ACGCACT5750.088.1802144
TGACGCC502.2584572E-887.06101145
GAGCCCC502.2584572E-887.06101145
CGCACTT5700.086.4121865
ATAAGCC608.258212E-1084.642654145
CAAGCCC353.4259603E-582.91525145
TAGAAAC6450.081.974082
TAGTAAC23850.081.38792
TAACAAG25950.074.8063355
Produced by FastQC (version 0.11.5)