FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_231_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_231_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1708761
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT60330.35306283324584303No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT50830.29746699509176533No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT33180.19417577999497881No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT30780.1801305156192118No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA25760.15075250429989917No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA25710.15045989462540402No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGT24000.14045264375767003No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA23120.13530271348655545No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCAGGCACTGAAG23060.1349515818771613No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA23010.13465897220266615No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC22720.1329618360905943No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGA22190.12986017354094576No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC21710.12705112066579233No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT20710.12119892717588944No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT20320.1189165717148273No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT20250.11850691817053409No Hit
GTTGTATTCAGTGCCGTTGCAGCAGGTTGGTTTCTGCATACTGTCCAATC19500.1141177730531069No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT19220.11247915887593407No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGA18820.11013828147997291No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC17840.10440313185986806No Hit
ATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG17770.10399347831557486No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC17490.10235486413840203No Hit
CTCCAATGTTGCTTTGCAACTCTTCTGCCAGCTTTTGGACGTCTTCTCCT17410.10188668865920979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA8550.0119.452182
CACGCAC8650.0118.074683
AGCACGC8600.0117.915441
GTAACAA10500.0113.8311544
CGCACTT10550.097.50525
CGGTGCA1350.096.5783542
ACGCACT10650.095.223674
GTATAAA1350.091.212891
CGCACCC406.097198E-790.68018145
ACGTGCA2000.083.2988362
CTCGGAA451.2288056E-680.604614145
GCTGCCC1300.078.124466145
CGACGAA755.4569682E-1177.380424145
GAAACAC4950.076.096494
GGTACCC300.00193130872.54415145
AAACACG5200.072.4422455
GCACTTT18800.069.753096
TAACAAG17250.069.712545
CTGGTCC554.048181E-665.94923145
GAGCCCC451.1905073E-464.48369145
Produced by FastQC (version 0.11.5)