FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_2018_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_2018_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1860301
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT104590.5622208449062813No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA94910.510186254805002No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC87740.471644104905604No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT85820.46132319447229236No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG82280.4422940158608741No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA69720.37477806010962744No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT69680.37456304114226674No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA58780.3159703725364874No Hit
GCTATGAACACAGCAAAAACAATGAATGGGATGGGAAAAGGAGAAGACGT58410.31398144708840126No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAACTAGGAAC57140.30715459487470037No Hit
GTAGTAACAAGAGCAACAAAAAAGAAGGGCCTGATTCTGGCTGAGAGAAA56070.3014028374978028No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT55250.2969949486669093No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT51280.27565431615636393No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT50470.27130018206731066No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAA49130.2640970466607286No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG47890.25743145867254813No Hit
TAGTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCA46370.24926073791284314No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA45610.24517537753299062No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC45090.24238013095730207No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT43550.23410190071391673No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT42410.22797386014413798No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT39310.21130989017368695No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG38490.20690200134279346No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT37040.19910756377596958No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC36840.1980324689391663No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT35440.19050680508154325No Hit
CCTTTATTCTTATTTTCATGTTTACTCCTCTTTTTATTTGATTTAAATGT34980.1880340869568957No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG34910.18765780376401453No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA34850.18733527531297353No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAACTGTGT34730.18669021841089156No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG34600.18599140676696943No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT33910.18228232957999807No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC33510.1801321399063915No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG33040.17760566703990374No Hit
GTGAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA32930.17701436487966196No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT31950.17174640017932583No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG30680.1649195479656249No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT29740.15986660223264945No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT28930.15551246814359612No Hit
AAATTATTCAATGCAAGTGAAACTAGGAACGCTCTGTGCTTTGTGCGAAA28470.15303975001894854No Hit
CAATACTTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGCCT27770.14927691809013702No Hit
GGATGTAATGGAAGTGCTAAAGCAGAGCTCTATGGGAAATTCAGCTCTTG27660.1486856159298952No Hit
GCTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA27600.14836308747885424No Hit
CTTCAGCAGAAAGCCCCTCAATTACTATGTGGTCACTCAATACTTCCATG27550.1480943137696534No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA27510.14787929480229275No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA27490.1477717853186124No Hit
TTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGATCTCAATACT26610.14304136803667794No Hit
GAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAGAATCTGGAATG26150.14056864991203036No Hit
ACATTGGAGTATTGAGATCCCTTGGGGCAAGTCAGAAGAATGGGGAAGGA26100.14029987620282955No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT25890.13917102662418607No Hit
GCGCTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTC25780.1385797244639443No Hit
GGAGTATTGAGATCCCTTGGGGCAAGTCAGAAGAATGGGGAAGGAATTGC25730.13831095075474345No Hit
ATTTAAATGTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAA25730.13831095075474345No Hit
CCCTTGGGGCAAGTCAGAAGAATGGGGAAGGAATTGCAAAGGATGTAATG25370.13637578004849754No Hit
GTGCCAGGAGTGAGACGGGAAATGCAGATGGTCTCAGCTATGAACACAGC25180.1353544399535344No Hit
TCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGT24650.13250543863600567No Hit
CTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCA24320.13073153215528024No Hit
CTCAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTT23360.12557107693862446No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA23190.12465724632734164No Hit
TTCATGAAGCATTTGAAATAGCAGAAGGCCATGAAAGCTCAGCGCTACTA23080.12406594416709985No Hit
ATCTGGAATGGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTT23030.12379717045789902No Hit
AGTCAGAAGAATGGGGAAGGAATTGCAAAGGATGTAATGGAAGTGCTAAA22830.12272207562109573No Hit
TAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAATGCAGA22690.12196950923533342No Hit
GGGAAAAGGAGAAGACGTCCAAAAACTGGCAGAAGAACTGCAAAGCAACA21750.11691656350235795No Hit
GCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAG21640.11632526134211614No Hit
CTCAATACTTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGC21570.115948978149235No Hit
GTATGAAAAGTTCCTCAAAGAGCAACTGTCCGAAATACAGTTGGATTGAT21270.11433633589403006No Hit
CAATAGGACATTTAAATCAAATAAAAAGAGGAGTAAACATGAAAATAAGA21080.11331499579906691No Hit
CCATAGAGCTCTGCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCC20940.11256242941330462No Hit
CTGTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTAC20810.11186361776938249No Hit
CCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGT20680.11116480612546034No Hit
CCTTTTCCCATCCCATTCATTGTTTTTGCTGTGTTCATAGCTGAGACCAT20590.11068101344889887No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA20430.10982093757945623No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT20130.1082082953242513No Hit
GTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTTATCAGCTC19820.10654189832720619No Hit
TCTTTAGCCTGAATTTCTTTTTGGTAACTGTGTCTCAAAATTGATACCTC19690.10584308668328404No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG19550.10509052029752174No Hit
CATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGG19500.10482174658832091No Hit
GTAGAAACAACAGCAACAAAAAAGAAGGGCCTGATTCTGGCTGAGAGAAA19490.10476799184648077No Hit
CTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTAGCACTTCCATTA19460.10460672762096027No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCAGCAGAAAGCCCCT19450.10455297287912009No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCAT19360.10406918020255862No Hit
GGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGTCAAGAATAAA19180.10310159484943565No Hit
GGACAATAGGACATTTAAATCAAATAAAAAGAGGAGTAAACATGAAAATA19140.102886575882075No Hit
CAATATGACCACAACACAAATTGAGGTGGGTCCGGGAGCAACCAATGCCA19080.102564047431034No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTC100.0070768525145.09927145
CGCCGTG100.0070768525145.09927145
GCACGCA3200.0122.216912
AGCACGC3250.0118.10821
GCCGACC256.444383E-6116.07941145
CGGTGCA650.0111.422822
CACGCAC3550.0110.173283
ATAAGCC750.096.73285145
GAACGCC700.093.27811145
GGCACCC406.0946695E-790.68705145
GGTGCAT800.090.535913
TCTCGCC259.3625364E-487.059555145
CTCGAGG259.3625364E-487.059555145
ACGTGCA950.083.860332
TGACGCC353.4264034E-582.91387145
GCAGCCC353.4264034E-582.91387145
TAGCTGG353.4264034E-582.91387145
GGAGCGT353.4548677E-582.7756963
CGGAGCG353.455789E-582.771242
GCGAGAA755.4569682E-1177.38628145
Produced by FastQC (version 0.11.5)