FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_2017_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_2017_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1828217
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA124760.6824135209332371No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT94940.5193037806781143No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT81600.44633651256935036No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC78970.4319509117353137No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG74240.4060787094748599No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA65160.3564128328311136No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT60580.3313611021011182No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG56070.3066922580853367No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA53280.2914314876188111No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAACTAGGAAC48640.26605156827663234No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT47980.2624414935426156No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAA45180.24712602497405944No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG42980.23509244252733677No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT42810.23416257479281724No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT41820.22874746269179205No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG41770.22847397218163928No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA40980.2241528221212252No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT39250.21469005046993875No Hit
GTAGAAACACGAGCGAAAGAAGAGTAAATCACCAGGTGGTAGCTCATCAG38430.210204806103433No Hit
GAACACAACTGACTTCTGAACAAAGTCCTCATGAAGTTTCCCCTGTCTCT37350.20429741108413277No Hit
ACAACAGCCAGTCAGAACACAACTGACTTCTGAACAAAGTCCTCATGAAG37140.20314875094149107No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT36160.19778833694249642No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT35890.19631148818767138No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG35650.194998733738938No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT35310.19313899826989903No Hit
GTCTCTAGAATGTGAGGGAGCGGTCCCACTTTATTTTCAGGATCAGTGAG35210.19259201724959346No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCAT33250.18187118925160414No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT33060.18083192531302356No Hit
GCTATGAACACAGCAAAAACAATGGATGGGATGGGAAAAGGAGAAGACGT32790.17935507655819852No Hit
CTTCTGAACAAAGTCCTCATGAAGTTTCCCCTGTCTCTAGAATGTGAGGG32650.1785893031297707No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA32330.17683896386479286No Hit
GTAGAAACACGTGCGAAAGAAGAGTAAATCACCAGGTGGTAGCTCATCAG32180.17601849233433448No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC31560.17262721000843992No Hit
GTAAATCACCAGGTGGTAGCTCATCAGCTGAATCTGGCAAAGACACAGGG31490.172244323294226No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG31110.1701657954170648No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC30400.16628223017289523No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAACTGTGT30210.16524296623431464No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC29960.1638755136835507No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT29520.16146879719420615No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT29210.15977315603125886No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT28660.1567647604195782No Hit
AAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAAC28370.15517851546069203No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG28240.15446744013429478No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA28150.15397515721601976No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT27950.1528811951754086No Hit
GGTGGTAGCTCATCAGCTGAATCTGGCAAAGACACAGGGGACGAGGGTGA27370.14970870525763624No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT27170.1486147432170251No Hit
ATTTAAATGTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAA26670.14587983811549723No Hit
GGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGTCAAGAATAAA26280.1437466121363055No Hit
GGTCATATGCATTCAATCTATGCAAAGTTGATAAGGACTTGTATCCATTG25840.14133989564696095No Hit
AAAGAAGAGTAAATCACCAGGTGGTAGCTCATCAGCTGAATCTGGCAAAG25670.14041002791244148No Hit
AAATTATTCAATGCAAGTGAAACTAGGAACGCTCTGTGCTTTGTGCGAAA25510.13953485827995254No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA25100.1372922360966997No Hit
GTATGAAAAGTTCCTCAAAGAGCAACTGTCCGAAATACAGTTGGATTGAT25000.1367452550763941No Hit
CAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATT24440.13368216136268288No Hit
GCTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA24240.13258819932207172No Hit
TTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGATCTCAATACT24010.1313301429753689No Hit
CCTTTATTCTTATTTTCATGTTTACTCCTCTTTTTATTTGATTTAAATGT23910.13078316195506332No Hit
GCGCTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTC23680.12952510560836047No Hit
GAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAGAATCTGGAATG23390.12793886064947432No Hit
GAGTAAATCACCAGGTGGTAGCTCATCAGCTGAATCTGGCAAAGACACAG23260.12722778532307707No Hit
GACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAA23010.12586033277231312No Hit
GTGCCAGGAGTGAGACGGGAAATGCAGATGGTCTCAGCTATGAACACAGC22490.12301603146672414No Hit
ATCTGGAATGGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTT22240.1216485789159602No Hit
CTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCA22040.12055461687534905No Hit
CAATATGACCACAACACAAATTGAGGTGGGTCCGGGAGCAACCAATGCCA21960.12011703205910458No Hit
AGTCAGAACACAACTGACTTCTGAACAAAGTCCTCATGAAGTTTCCCCTG21620.11825729659006562No Hit
GTAGAAACAACAGCCAGTCAGAACACAACTGACTTCTGAACAAAGTCCTC21590.11809320228397394No Hit
TTCATGAAGCATTTGAAATAGCAGAAGGCCATGAAAGCTCAGCGCTACTA21420.11716333454945448No Hit
GAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAACTG21290.11645225922305721No Hit
GTGGTAGCTCATCAGCTGAATCTGGCAAAGACACAGGGGACGAGGGTGAT20920.11442842944792658No Hit
TCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGT20830.11393614652965157No Hit
TGACTACCCTGGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGT20210.110544864203757No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT20080.10983378887735974No Hit
ACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAAT20030.10956029836720696No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA19930.10901331734690138No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG19760.10808344961238191No Hit
GCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAG19270.10540324261288458No Hit
CAACTGACTTCTGAACAAAGTCCTCATGAAGTTTCCCCTGTCTCTAGAAT19270.10540324261288458No Hit
GTCAGAACACAACTGACTTCTGAACAAAGTCCTCATGAAGTTTCCCCTGT19140.10469216728648734No Hit
GTTTTTGCTGTGTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCC19120.10458277108242621No Hit
CCATTTATGTTACAGGATGAGGACTCCAACCAGTGTCTTGCCCCAGAAGA19070.10430928057227343No Hit
CTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTAGCACTTCCATTA19040.10414518626618174No Hit
CAATACTTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGCCT18840.10305122422557059No Hit
CTTCAGCAGAAAGCCCCTCAATTACTATGTGGTCACTCAATACTTCCATG18820.10294182802150949No Hit
CCATAGAGCTCTGCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCC18810.10288712991947892No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAAATAGAAGTA18750.10255894130729558No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCAGCAGAAAGCCCCT18740.10250424320526502No Hit
GGATGTAATGGAAGTGCTAAAGCAGAGCTCTATGGGAAATTCAGCTCTTG18630.1019025640829289No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTTCTA18550.10146497926668444No Hit
CCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGT18370.10048041343013439No Hit
CTCAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTT18310.10015222481795105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCC400.0145.13194145
CGGAGAC100.007113298144.850133
GCAGCCC256.4371416E-6116.10555145
CAGCGCC203.852609E-4108.84895145
ATCCTAC800.0108.6583947
GCGAGAA750.096.75462145
AGCACGC2750.094.810991
GCACGCA2800.093.117942
CACGCAC2900.092.404393
TAGTAAC12200.090.2345052
TCTCGGG900.088.69174145
GGCACCC259.354139E-487.07916145
GCCTCCC750.087.07916145
GTGACCC259.354139E-487.07916145
TATCCTA1000.086.921976
CGAGCCA259.424598E-486.9148255
GTAGTAA13300.083.316051
AACGGCC353.422563E-582.93254145
AACACGT6650.081.693576
ACACGTG6700.081.0883567
Produced by FastQC (version 0.11.5)