FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_2017_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_2017_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1839939
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA125180.6803486419930226No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT95340.5181693523535291No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT81800.44457995618332996No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC79310.43104689883740716No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG74500.40490472781978093No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA65440.355663964946664No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT60720.3300109405800953No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG56280.3058797057945943No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA53480.29066180998391794No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAACTAGGAAC48840.2654435826405115No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT48130.2615847590599471No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAA45360.2465299121329566No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG43170.2346273436238919No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT43040.23392079846125333No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT41980.22815973790435443No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG41910.22777929050908755No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA41130.22354001953325628No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT39370.2139744850236883No Hit
GTAGAAACACGAGCGAAAGAAGAGTAAATCACCAGGTGGTAGCTCATCAG38620.20989826293154284No Hit
GAACACAACTGACTTCTGAACAAAGTCCTCATGAAGTTTCCCCTGTCTCT37470.2036480557235865No Hit
ACAACAGCCAGTCAGAACACAACTGACTTCTGAACAAAGTCCTCATGAAG37260.20250671353778577No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT36230.19690870186457268No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT36020.19576735967877196No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG35790.1945173182371807No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT35430.19256073163295087No Hit
GTCTCTAGAATGTGAGGGAGCGGTCCCACTTTATTTTCAGGATCAGTGAG35320.19196288572610287No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCAT33420.1816364564260011No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT33120.18000596758914292No Hit
GCTATGAACACAGCAAAAACAATGGATGGGATGGGAAAAGGAGAAGACGT33000.17935377205439962No Hit
CTTCTGAACAAAGTCCTCATGAAGTTTCCCCTGTCTCTAGAATGTGAGGG32700.17772328321754144No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA32450.17636454252015962No Hit
GTAGAAACACGTGCGAAAGAAGAGTAAATCACCAGGTGGTAGCTCATCAG32310.1756036477296258No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC31660.17207092191643308No Hit
GTAAATCACCAGGTGGTAGCTCATCAGCTGAATCTGGCAAAGACACAGGG31610.17179917377695672No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG31240.16978823754483166No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC30570.1661468124758484No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAACTGTGT30320.16478807177846658No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC30030.16321193256950367No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT29570.16071184968632113No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT29310.15929875936104404No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT28740.1562008305710135No Hit
AAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAAC28480.1547877402457364No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG28320.15391814619941205No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA28240.15348334917624987No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT28070.15255940550203023No Hit
GGTGGTAGCTCATCAGCTGAATCTGGCAAAGACACAGGGGACGAGGGTGA27500.1494614767119997No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT27300.14837448415409424No Hit
ATTTAAATGTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAA26740.14533090499195897No Hit
GGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGTCAAGAATAAA26330.1431025702482528No Hit
GGTCATATGCATTCAATCTATGCAAAGTTGATAAGGACTTGTATCCATTG25920.14087423550454664No Hit
AAAGAAGAGTAAATCACCAGGTGGTAGCTCATCAGCTGAATCTGGCAAAG25850.1404937881092797No Hit
AAATTATTCAATGCAAGTGAAACTAGGAACGCTCTGTGCTTTGTGCGAAA25580.13902634815610734No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA25190.1369067126681917No Hit
GTATGAAAAGTTCCTCAAAGAGCAACTGTCCGAAATACAGTTGGATTGAT25060.13620016750555317No Hit
CAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATT24570.1335370357386848No Hit
GCTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA24300.13206959578551247No Hit
TTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGATCTCAATACT24140.13120000173918808No Hit
CCTTTATTCTTATTTTCATGTTTACTCCTCTTTTTATTTGATTTAAATGT24030.13060215583234008No Hit
GCGCTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTC23790.12929776476285354No Hit
GAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAGAATCTGGAATG23470.12755857667020482No Hit
GAGTAAATCACCAGGTGGTAGCTCATCAGCTGAATCTGGCAAAGACACAG23340.12685203150756627No Hit
GACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAA23120.1256563396938703No Hit
GTGCCAGGAGTGAGACGGGAAATGCAGATGGTCTCAGCTATGAACACAGC22640.1230475575548972No Hit
ATCTGGAATGGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTT22280.1210909709506674No Hit
CTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCA22110.12016702727644776No Hit
CAATATGACCACAACACAAATTGAGGTGGGTCCGGGAGCAACCAATGCCA22090.1200583280206572No Hit
GTAGAAACAACAGCCAGTCAGAACACAACTGACTTCTGAACAAAGTCCTC21670.11777564364905575No Hit
AGTCAGAACACAACTGACTTCTGAACAAAGTCCTCATGAAGTTTCCCCTG21670.11777564364905575No Hit
TTCATGAAGCATTTGAAATAGCAGAAGGCCATGAAAGCTCAGCGCTACTA21550.11712344811431248No Hit
GAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAACTG21370.11614515481219757No Hit
GTGGTAGCTCATCAGCTGAATCTGGCAAAGACACAGGGGACGAGGGTGAT20980.11402551932428195No Hit
TCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGT20880.11348202304532923No Hit
TGACTACCCTGGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGT20290.11027539499950814No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT20130.10940580095318378No Hit
ACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAAT20070.10907970318581213No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA20010.10875360541844051No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG19830.1077753121163256No Hit
CAACTGACTTCTGAACAAAGTCCTCATGAAGTTTCCCCTGTCTCTAGAAT19380.10532957886103833No Hit
GCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAG19330.10505783072156197No Hit
GTCAGAACACAACTGACTTCTGAACAAAGTCCTCATGAAGTTTCCCCTGT19210.1044056351868187No Hit
CCATTTATGTTACAGGATGAGGACTCCAACCAGTGTCTTGCCCCAGAAGA19190.10429693593102815No Hit
GTTTTTGCTGTGTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCC19160.10413388704734233No Hit
CTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTAGCACTTCCATTA19070.10364474039628489No Hit
CCATAGAGCTCTGCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCC18930.10288384560575106No Hit
CAATACTTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGCCT18900.10272079672206523No Hit
CTTCAGCAGAAAGCCCCTCAATTACTATGTGGTCACTCAATACTTCCATG18880.1026120974662747No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAAATAGAAGTA18810.10223165007100779No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCAGCAGAAAGCCCCT18780.10206860118732197No Hit
GGATGTAATGGAAGTGCTAAAGCAGAGCTCTATGGGAAATTCAGCTCTTG18700.10163380416415978No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTTCTA18650.10136205602468341No Hit
CCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGT18430.10016636421098743No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACAT100.0071151946144.837235
AGCACGC2800.0126.7222441
GCACGCA2950.0120.278742
TTGCGCC256.4360356E-6116.109604145
CACGCAC3250.0109.176093
TAGTAAC14600.098.699522
TGACGCC900.096.75801145
GCCTCCC850.093.912186145
GTAGTAA15950.090.799641
ATGCCCC353.4219767E-582.93543145
GAAACAC19550.081.4918754
AACACGT8650.078.7212146
ACACGTG8750.077.8215567
ATCCTAC755.638867E-1177.269627
AAGCGGA851.8189894E-1276.672282
GTACTCC406.633559E-572.568504145
CAAGCCC300.00192876172.568504145
GGGCCCC300.00192876172.568504145
TATCCTA801.0004442E-1072.440286
GCGTGAG300.001945275872.412711
Produced by FastQC (version 0.11.5)