FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_2016_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_2016_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881005
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA84890.4513012990396092No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT56520.30047767018163163No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG55790.2965967660904676No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC50210.2669317731744466No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA47530.25268407048359787No Hit
TCATAATCCTCTGCTGTGTCCCTCCCAAAGAAGAAATTGGGGATGGTCTG44480.23646933421229607No Hit
CAGTTATAGTGTCTATTTTGTTGCTTTAATAATCGAGGTCATCATAATCC42690.22695314472848288No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT41120.21860654277899316No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA40520.2154167585944748No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG40000.21265227896789218No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG39230.20855872259776023No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT37940.20170068660104573No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC37270.19813876092833355No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT35620.18936685442090798No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA35530.1888883867932302No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA31280.16629408215289165No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC29810.15847911090082165No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT29150.15497034829785142No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT29030.15433239146094774No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG28900.1536412715543021No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT28290.15039832430004174No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG27680.14715537704578138No Hit
ATATTGAGGGACGTGATGCAGATGTCAAAGGAAATCTACTCAAGATGATG27320.14524150653507034No Hit
CCTTTAATGCAGGAGAATTTTCTCTCCCCACTTTTGATTCATTGAACATT26710.14199855928080998No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAGCA26670.1417859070018421No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC26210.13934040579371135No Hit
GAATAAACACCCACATTCCAAACGTATTGAGACAATCACAGTTATAGTGT25320.13460889258667574No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC24000.12759136738073532No Hit
GGTCATATGCATTCAATCTATGCAAAGTTGATAAGGACTTGTATCCATTG23990.12753820431099333No Hit
GTTATAGTGTCTATTTTGTTGCTTTAATAATCGAGGTCATCATAATCCTC23670.12583698607925017No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT23420.12450790933570086No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT23200.12333832180137745No Hit
CCATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAA23060.12259403882498984No Hit
CTACAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAG22750.12094598366298867No Hit
TTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTATACTG22510.11967006998918131No Hit
CCATATAAACAATAAAAATAGCTAGCATTAATGTTACAGCCAAACTAGAA22330.1187131347338258No Hit
GTTTATATGGTCTCCAGAGACAACGTTTCATGCTCCATCTGTCTATAAGG22310.11860680859434186No Hit
ATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTAAT22270.11839415631537395No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA22180.11791568868769621No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA22010.11701191650208267No Hit
GAACATTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTA22000.11695875343234069No Hit
GACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAA20230.10754889008801145No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG20220.10749572701826948No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAACTAGGAAC19930.10595399799575227No Hit
CTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAACA19900.10579450878652635No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA19750.10499706274039676No Hit
GCATTAATGTTACAGCCAAACTAGAAGCAGCAGTTGAGTAATAGAGCAGT19250.10233890925329811No Hit
ACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAAT19190.10201993083484627No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT19130.10170095241639444No Hit
CTTTTGATTCATTGAACATTACTGCTGCATCTTTAAATGATGATGGATTG19050.10127564785845865No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAA151.2265725E-4145.10683145
AACGGCC151.2265725E-4145.10683145
TCGCGCC151.2265725E-4145.10683145
AGACACG100.0070757587145.10681145
CGCACCC301.097269E-7120.92235145
AGCACGC7750.0114.931961
GCACGCA7850.0113.470882
GGCACCC1300.0111.620636145
GGCAACC203.8552808E-4108.83011145
GTCGGCC352.750603E-7103.64773145
CACGCAC8600.0103.5751653
GCTGCCC1400.098.46535145
TGACGCC1250.092.86837145
GCCGACC1100.085.74494145
CGCACTT10650.085.682665
ACGCACT10850.085.438244
GCGGTGC1300.083.557481
CGGTGCA801.8189894E-1281.47072
TCTAAAT1100.079.001892
GAAACAC5950.076.682394
Produced by FastQC (version 0.11.5)