FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_2015_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_2015_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812574
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA173750.9585815530841776No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG107600.5936309358955828No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA98100.5412192826334263No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG89190.4920626688896564No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC85830.47352549468325156No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC82650.4559813833807613No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAGCA81020.44698864708420183No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA77850.4294997059430401No Hit
AGTAGAAACAACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTC75920.41885186480662306No Hit
CCTTTAATGCAGGAGAATTTTCTCTCCCCACTTTTGATTCATTGAACATT69980.38608078897744313No Hit
CTACAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAG58500.3227454437722267No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT54960.3032152066619073No Hit
CCATATAAACAATAAAAATAGCTAGCATTAATGTTACAGCCAAACTAGAA53760.296594787302477No Hit
TTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTATACTG50870.2806506106785157No Hit
CTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAACA50210.2770093800308291No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT45100.24881742759192177No Hit
GAACATTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTA44560.24583823888017814No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC43900.24219700823249146No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT43300.23888679855277634No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT42940.23690067274494725No Hit
CCATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAA42890.2366248219383043No Hit
CAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG42320.23348012274257493No Hit
CTTTTGATTCATTGAACATTACTGCTGCATCTTTAAATGATGATGGATTG41340.22807344693237352No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG41120.22685970338314462No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT40880.22553561951125858No Hit
ATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTAAT40280.22222540983154343No Hit
CCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAAC40170.221618538056929No Hit
GGATAGCTGCTGGCACCTTTAATGCAGGAGAATTTTCTCTCCCCACTTTT39160.21604635176274184No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA38230.21091552675918335No Hit
GCTTAGACAGGATAGCTGCTGGCACCTTTAATGCAGGAGAATTTTCTCTC38220.21086035659785476No Hit
ATACAAGGCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGTTGTCT38050.2099224638552688No Hit
CTTTAATGCAGGAGAATTTTCTCTCCCCACTTTTGATTCATTGAACATTA37260.20556402111031052No Hit
GGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGT36640.20214347110793823No Hit
GCATTAATGTTACAGCCAAACTAGAAGCAGCAGTTGAGTAATAGAGCAGT35960.1983919001375944No Hit
CAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAGCAT34980.19298522432739298No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT34880.19243352271410713No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT34790.19193699126214986No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT34450.19006120577697794No Hit
TCCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAA33630.1855372525480339No Hit
CTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTAATGCTAGCTA32780.1808477888351041No Hit
GTCCCTCTGCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAA32140.17731689851007462No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG31200.17213090334518757No Hit
TCTTTGTAATGATGACAAGCAAACAAGCACTACAATAAAGGAAAATACAA29600.16330367753261385No Hit
GGATAACCATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGG29350.16192442349939917No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT28260.15591087591458336No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA27670.1526558363961968No Hit
CTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTA27440.15138692268563933No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC27350.15089039123368206No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG27040.14918011623249589No Hit
GCTATGAACACAGCAAAAACAATGAATGGGATGGGAAAAGGAGAAGACGT26610.1468077992953667No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT26520.14631126784340942No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT26430.14581473639145215No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT26150.14426997187425175No Hit
CTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTAGACATAGG25810.14239418638907986No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAA25720.14189765493712259No Hit
TGCTTAGACAGGATAGCTGCTGGCACCTTTAATGCAGGAGAATTTTCTCT25700.1417873146144654No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAACTAGGAAC25320.13969084848397914No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA24690.13621512832027824No Hit
CTGCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAAC24090.13290491864056309No Hit
CATTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTATAC23660.13053260170343392No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC23570.13003607025147665No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT23320.12865681621826197No Hit
AACAAGCACTACAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGAC23220.12810511460497614No Hit
GAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAAGA23050.12716722186239016No Hit
AAACAACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTG22840.12600864847448986No Hit
CAATAAAAATAGCTAGCATTAATGTTACAGCCAAACTAGAAGCAGCAGTT22800.1257879678291755No Hit
GGCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAG22260.12280877911743189No Hit
CATTAATGCTAGCTATTTTTATTGTTTATATGGTCTCCAGAGACAACGTT21920.12093299363225998No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT20930.11547114766073No Hit
GCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTAATGCTAG20300.11199542749702909No Hit
TCTTTAAATGATGATGGATTGGATAACCATACTATACTGCTCTATTACTC20100.11089202427045737No Hit
CATATAAACAATAAAAATAGCTAGCATTAATGTTACAGCCAAACTAGAAG19810.10929208959192839No Hit
CAATACTTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGCCT19760.10901623878528546No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT19590.1080783460426995No Hit
CTCAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTT18960.1046026258789986No Hit
AAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAAC18750.1034440524910983No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG18690.10311303152312679No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT18550.10234064926452659No Hit
CTTCGAAACCAAACACAAATGCAACCAGACCTGCTTAGACAGGATAGCTG18510.10211996861921224No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG18440.10173377748991214No Hit
GCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGA18140.10007867265005457No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCC100.0070745503145.11496145
AGCACGC4550.0122.535551
CGGTGCA301.1105112E-7120.678952
GCACGCA4650.0119.9003752
CACGCAC4700.0118.6281053
GCTAGCC850.0110.97027145
GCTGCCC1050.0110.56378145
GGCACCC501.9463187E-10101.580475145
CGCACCC607.2759576E-1296.74331145
GTGGGGG554.129106E-1092.34589145
TGACGCC950.091.65155145
GCGAGAA259.358473E-487.06898145
TAGAAAC21400.083.236933
GCGGTGC1250.081.0962451
TGGAGCC451.2274922E-680.61942145
GGTGTAA554.372305E-879.15362145
ACGTGCA1300.077.9771652
GAGCAGA1050.075.855341
TAGTAAC38750.075.490522
ACAACGC23650.075.342938
Produced by FastQC (version 0.11.5)