FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_2015_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_2015_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1824906
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA174460.955994445741315No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG108150.5926332643982759No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA98640.5405209912181779No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG89460.49021703035663206No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC86030.47142154171228545No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC83050.4550919335023283No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAGCA81350.4457763851946347No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA78280.42895360089779966No Hit
AGTAGAAACAACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTC76220.41766534824259444No Hit
CCTTTAATGCAGGAGAATTTTCTCTCCCCACTTTTGATTCATTGAACATT70270.38506092916566664No Hit
CTACAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAG58820.3223179714462005No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT55230.30264572531407097No Hit
CCATATAAACAATAAAAATAGCTAGCATTAATGTTACAGCCAAACTAGAA54050.29617963884167187No Hit
TTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTATACTG51110.28006922000366047No Hit
CTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAACA50460.2765073927095423No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT45250.24795797701361055No Hit
GAACATTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTA44680.24483452846338388No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC44140.24187547194211645No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT43560.23869722604890334No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT43130.23634094030048672No Hit
CCATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAA43120.23628614295750028No Hit
CAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG42510.23294350503532785No Hit
CTTTTGATTCATTGAACATTACTGCTGCATCTTTAAATGATGATGGATTG41480.22729937870772524No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG41380.22675140527786086No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT41160.22554586373215937No Hit
ATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTAAT40460.221710049723109No Hit
CCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAAC40370.22121687363623113No Hit
GGATAGCTGCTGGCACCTTTAATGCAGGAGAATTTTCTCTCCCCACTTTT39340.21557274730862852No Hit
GCTTAGACAGGATAGCTGCTGGCACCTTTAATGCAGGAGAATTTTCTCTC38450.21069578378283593No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA38390.21036699972491732No Hit
ATACAAGGCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGTTGTCT38200.2093258502081751No Hit
CTTTAATGCAGGAGAATTTTCTCTCCCCACTTTTGATTCATTGAACATTA37470.20532564417016547No Hit
GGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGT36800.20165422219007445No Hit
GCATTAATGTTACAGCCAAACTAGAAGCAGCAGTTGAGTAATAGAGCAGT36060.1975992188090784No Hit
CAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAGCAT35170.19272225528328582No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT35050.19206468716744862No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT34920.19135232170862498No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT34690.19009198281993703No Hit
TCCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAA33750.1849410325792123No Hit
CTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTAATGCTAGCTA32860.18006406905341973No Hit
GTCCCTCTGCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAA32290.17694062050319306No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG31310.17157048089052257No Hit
TCTTTGTAATGATGACAAGCAAACAAGCACTACAATAAAGGAAAATACAA29690.16269331132672038No Hit
GGATAACCATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGG29450.16137817509504598No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT28390.15556965673848405No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA27830.1525010055312438No Hit
CTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTA27550.15096667992762366No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC27430.15030911181178647No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG27210.14910357026608492No Hit
GCTATGAACACAGCAAAAACAATGAATGGGATGGGAAAAGGAGAAGACGT26770.14669248717468186No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT26660.1460897164018311No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT26520.14532255360002105No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT26290.14406221471133307No Hit
CTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTAGACATAGG25900.14192511833486218No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAA25820.14148673959097072No Hit
TGCTTAGACAGGATAGCTGCTGGCACCTTTAATGCAGGAGAATTTTCTCT25790.14132234756201142No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAACTAGGAAC25410.13924004852852695No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA24830.13606180263531384No Hit
CTGCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAAC24190.1325547726841821No Hit
CATTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTATAC23730.13003409490680617No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC23690.12981490553486044No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT23400.12822578258825384No Hit
AACAAGCACTACAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGAC23350.1279517958733217No Hit
GAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAAGA23100.12658186229866086No Hit
AAACAACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTG22910.12554071278191864No Hit
CAATAAAAATAGCTAGCATTAATGTTACAGCCAAACTAGAAGCAGCAGTT22880.12537632075295932No Hit
GGCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAG22390.1226912509466241No Hit
CATTAATGCTAGCTATTTTTATTGTTTATATGGTCTCCAGAGACAACGTT22040.12077334394209893No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT21060.11540320432942848No Hit
GCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTAATGCTAG20380.11167698500635101No Hit
TCTTTAAATGATGATGGATTGGATAACCATACTATACTGCTCTATTACTC20170.1105262408036359No Hit
CAATACTTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGCCT19870.10888232051404292No Hit
CATATAAACAATAAAAATAGCTAGCATTAATGTTACAGCCAAACTAGAAG19860.10882752317105648No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT19730.10811515771223285No Hit
CTCAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTT19010.10416974901720966No Hit
AAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAAC18810.10307380215748099No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG18810.10307380215748099No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT18640.10214224732671162No Hit
CTTCGAAACCAAACACAAATGCAACCAGACCTGCTTAGACAGGATAGCTG18600.10192305795476589No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG18530.10153947655386086No Hit
GCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGA18250.10000515095024073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCACCC253.695277E-8145.11447145
AATCGCC100.0070746257145.11447145
ACGTAGG100.0071089314144.879878
CCACCCG100.007111261144.8645
TGACGCC900.0137.05257145
CGCACCC403.274181E-11126.97517145
AGCACGC5300.0122.9977341
CACGCAC5500.0119.8420263
GCACGCA5500.0119.8420262
GCCGACC501.9463187E-10101.58013145
CGGTGCA650.0100.290462
GAAACAC3000.098.9903954
GCAGCCC301.5935573E-596.74298145
AAACACG3100.095.797155
ATAAGCC1100.092.34558145
TCCAGGG259.358609E-487.06869145
CTCGCCC259.358609E-487.06869145
CGGAGCG259.4230595E-486.91845
GTGGGGG353.4246055E-582.92256145
GGCAACC353.4246055E-582.92256145
Produced by FastQC (version 0.11.5)