FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_2012_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_2012_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1491089
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC90660.6080119966011419No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT88540.5937942000779297No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATGGATAAG79070.5302835712690523No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG76270.5115053494459418No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT66540.4462510286106329No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA65060.4363253970755602No Hit
GGTTTATGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA60720.4072191532497389No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT57700.3869654997119555No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC55630.37308302857844167No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTG55060.36926031913587987No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC47820.3207052027075513No Hit
GCATAAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAATGTAA47150.31621184248559275No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT44160.2961593841816283No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTA42280.2835511495289684No Hit
CCTTATTTCCTCAAATTTCCGTCCCAATTGTTCCCGCCACTTCTCATTTC39370.26403521184852147No Hit
ACATAATGGAATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTT39340.2638340166147024No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT37040.2484090486885759No Hit
GTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT36570.24525699002541096No Hit
CTTTCAATCTGTGCCGTATTTCTTCAATTAACCACCTTATTTCCTCAAAT36540.24505579479159192No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG36230.24297677737546183No Hit
GCTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA34580.23191103951541456No Hit
GAAGAGGCAACACCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGG33800.22667996343611949No Hit
TCTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCT33590.22527159679938624No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT33140.2222536682921006No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC31390.21051727965265654No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT30980.20776761145712966No Hit
CCCTCGAGGAAATGTCACGAGACTGGTTTATGCTTATGCCTAGGCAAAAG29470.19764078468823792No Hit
ATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTATCTTTT29280.196366548207384No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGTATTTCTTCAATTAACCA29100.19515937680446974No Hit
CTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT29000.19448872602507294No Hit
ACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCC28790.19308035938833967No Hit
GTGCCGTATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCCGTCCCA28470.1909342768942699No Hit
CTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT28390.19039775627075245No Hit
GTATAATCTTCAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT28380.19033069119281276No Hit
GTACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGG27410.18382537863266377No Hit
GGCATAAGCATAAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGA27160.18214875168417177No Hit
TGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA26950.1807403850474385No Hit
GATAAGAACATAGTACTGGAAGCAAACTTCAGTATAATCTTCAACCGATT26610.17846017239748935No Hit
CTCTTGTTGGGAAACAAATTGTGGAATGGATTTTGAAAGAGGAATCCAGC26600.17839310731954966No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGCTC26540.1779907168519116No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA26520.17785658669603222No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCTTCTCCAAGCG26490.17765539146221318No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA26430.1772530009945751No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAA26420.17718593591663542No Hit
GCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG26340.17664941529311798No Hit
GTATCAAGGTCTCTAATCGGTTGAAGATTATACTGAAGTTTGCTTCCAGT25720.17249138046085782No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT25530.17121714398000387No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT25440.17061355827854677No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC25220.16913812656387378No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAGC24990.16759562977126113No Hit
TTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTTATGCTTATG23630.15847477917146463No Hit
ATTCTCTGTCGCTTTCAATCTGTGCCGTATTTCTTCAATTAACCACCTTA23530.1578041283920678No Hit
GCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGACC22790.15284131262453146No Hit
ATCTTCAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA22700.15223772692307436No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA22650.15190240153337592No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC22500.15089642536428072No Hit
GTACTGGAAGCAAACTTCAGTATAATCTTCAACCGATTAGAGACCTTGAT22110.1482808873246332No Hit
CTCCCATTCTCATCACAGCTTCTCCAAGCGAATCTCTGTATATTTTCAGA22100.14821382224669352No Hit
GTTTTCTTTGGCATATTCGCAAGCGATTTGCAGACAATGGATTGGGTGAT22040.14781143177905545No Hit
GTTGAAGATTATACTGAAGTTTGCTTCCAGTACTATGTTCTTATCCATGA21480.14405578741443334No Hit
GAACATAGTACTGGAAGCAAACTTCAGTATAATCTTCAACCGATTAGAGA21300.1428486160115191No Hit
CTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG21300.1428486160115191No Hit
TCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG20890.14009894781599222No Hit
CATCTGTACCTACTTCGCGTTACATTTCTGACATGACCCTCGAGGAAATG20820.13962949227041443No Hit
GTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGATCCAGC20690.13875764625719858No Hit
AAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAGCTTTC20600.13815406055574148No Hit
CTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGT20530.13768460501016372No Hit
ATACTGAAGTTTGCTTCCAGTACTATGTTCTTATCCATGACCGCCTGGTC20460.13721514946458596No Hit
TCTCTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCACTCAAGTC20450.13714808438664627No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCACTCAAGTCCTCCG20430.1370139542307669No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT20320.13627623837343042No Hit
GTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAA20290.13607504313961138No Hit
CTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCACTCAAGTCCTC20250.13580678282785266No Hit
ACATAGTACTGGAAGCAAACTTCAGTATAATCTTCAACCGATTAGAGACC20100.13480080665875746No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA19880.13332537494408447No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC19730.13231939877498927No Hit
TTCTAAGTGTCTCGCTGGATTCCTCTTTCAAAATCCATTCCACAATTTGT19590.13138048768383376No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT19550.13111222737207504No Hit
GTACCTACTTCGCGTTACATTTCTGACATGACCCTCGAGGAAATGTCACG19430.13030744643679887No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC19330.12963679565740208No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC19310.1295026655015227No Hit
CATCACAGCTTCTCCAAGCGAATCTCTGTATATTTTCAGAGACTCGAACC19060.1278260385530307No Hit
GTCCTACATTGTGGAAACATCTAATTCAGACAATGGAACGTGTTACCCAG18980.12728951792951326No Hit
GTCATGGATAAGAACATAGTACTGGAAGCAAACTTCAGTATAATCTTCAA18850.12641767191629744No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACG18800.126082346526599No Hit
GTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATAAGCA18770.12588115129277996No Hit
ATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTCGCAAGCGATT18560.12447278465604669No Hit
GGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAAG18130.12158898630464042No Hit
ATACAGAGATTCGCTTGGAGAAGCTGTGATGAGAATGGGAGACCTTCACT18070.12118659583700234No Hit
GTATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCCGTCCCAATTGT18000.1207171402914246No Hit
CATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTGGAATGGATTTTGA18000.1207171402914246No Hit
GATCAAAAGTCCTTAAAAGGAAGAGGCAACACCCTTGGCCTCGACATCAA17940.12031474982378651No Hit
GTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAAGCTGTGATGA17850.11971116412232939No Hit
GCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGAT17850.11971116412232939No Hit
CAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTG17630.11823573240765642No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC17560.11776627686207865No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC17450.11702856100474217No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTATCCAAAATGCC17420.11682736577092313No Hit
TCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGCTCTACTTCAA17380.1165591054591644No Hit
CAGGTAGACTGTTTTCTTTGGCATATTCGCAAGCGATTTGCAGACAATGG17160.11508367374449145No Hit
CTTCCAGGACATACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCAT17090.11461421819891368No Hit
CACTTAGAATGACAATTGCATCTGTACCTACTTCGCGTTACATTTCTGAC16990.11394356741951688No Hit
GATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATGGATAAGA16980.1138765023415772No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC16970.11380943726363751No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTGAAGATTATACTGAAGTTT16850.11300465632836136No Hit
CTTCAGTATAATCTTCAACCGATTAGAGACCTTGATACTACTAAGGGCTT16820.11280346109454231No Hit
CCTTAAAAGGAAGAGGCAACACCCTTGGCCTCGACATCAAAACAGCCACT16790.11260226586072328No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT16780.11253520078278359No Hit
CTTTCAGGTAGACTGTTTTCTTTGGCATATTCGCAAGCGATTTGCAGACA16690.11193161508132646No Hit
GACTCGAACCGTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAA16520.11079150875635191No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT16330.10951727227549797No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC16290.10924901196373925No Hit
CCACAGCACTCTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGC16210.1087124913402218No Hit
CTATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAAT16190.10857836118434246No Hit
TCCCAATTGTTCCCGCCACTTCTCATTTCTGCTCTGGAGGTAGTGAAGGT16100.10797477548288534No Hit
TTCCAGTACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAA15960.1070358643917298No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG15940.10690173423585043No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT15900.10663347392409171No Hit
GTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGCCGTATTTCTTCAATT15690.10522510728735843No Hit
AGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTC15670.10509097713147908No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG15660.10502391205353939No Hit
GTAGTATCAAGGTCTCTAATCGGTTGAAGATTATACTGAAGTTTGCTTCC15300.10260956924771089No Hit
GGACATACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGG15300.10260956924771089No Hit
CTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACC15290.10254250416977123No Hit
GAATGGGAGACCTTCACTACCTCCAGAGCAGAAATGAGAAGTGGCGGGAA14950.10026229151982209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA600.0132.99377145
TGTAACC301.0981785E-7120.903435145
TCACGAA501.9463187E-10101.558876145
ACCTGCC301.5951173E-596.72275145
CCTCGCC607.2759576E-1296.72275145
GCGAAAG4800.095.080542
GAGCGAA950.091.506091
ACACCCC651.6370905E-1189.28253145
GGTGTCC259.3660544E-487.05046145
GGACACC259.3660544E-487.05046145
ACTCAAC259.3660544E-487.05046145
CGAAAGC5300.086.110683
TGTACCC950.083.99607145
GTAGAAA12700.083.850561
TGACGCC1050.082.905205145
ATTGGGG2800.082.905205145
CGACGAA1100.079.136795145
GCTGGCC554.3783075E-879.136795145
GCAGTTT554.3783075E-879.136795145
TAGAAAC14600.072.938512
Produced by FastQC (version 0.11.5)