FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_1210_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_1210_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1698914
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG64970.3824207699742306No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT49670.2923632391045103No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA46570.27411628840541663No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG39120.23026474559630447No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT36940.2174330189756515No Hit
GTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCATAA31170.18347014622282234No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG30970.18229292359707436No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA30890.18182203454677517No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC30190.17770175535665725No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG29740.1750530044487243No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA29080.17116816978375596No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT28970.17052069733959457No Hit
CTGTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCAT28730.16910803018869702No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG25740.15150855193376475No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG25110.14780030066265862No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA24680.14526927201730044No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG24440.1438566048664029No Hit
GAATAAAGACTCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT24420.14373888260382808No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA24080.14173760414005654No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCATAAATAGGAGTA22270.13108373937703732No Hit
CAATTGGACCGATTACCCTTCAACACCAGAGAGGTGCCTTGATGACATAG21770.12814068281266738No Hit
GGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTT20980.12349065344096288No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT20800.12243115307778969No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC20760.12219570855264009No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA20090.11825201275638436No Hit
GAGCCATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAA19770.1163684565551876No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC19150.11271906641536888No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT19010.1118950105773453No Hit
GTTGTATTCAGTGCCGTTGCAGCAGGTTGGTTTCTGCATACTGTCCAATC18850.1109532324767469No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAGAGAAAGCTGAA18730.11024689890129812No Hit
GTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTCCATTTAC18550.10918739853812494No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA18490.10883423175040055No Hit
GTTTATTATTCCTTCATTGGAAAGCAGGACTGCGAGTTCTATTTGTGAGC18060.10630320310504239No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT17930.10553800839830621No Hit
ATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTC17860.10512598047929442No Hit
CCTTAATACTATTTCAGTTGGGCCATCAACATCCTCTGGTTCTTCCTCTA17530.10318356314681025No Hit
TCTTCATCCTCCACTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAG17380.10230064617749927No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC17280.10171203486462528No Hit
GTCCGGGAGCAACCAATGCCACCATAAACTTTGAAGCAGGAATTCTGGAG17170.10106456242046391No Hit
CATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCCACTGATGAT17080.10053481223887731No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACCC151.2269337E-4145.09547145
CGGCCTT100.007077352145.09547145
TCCGACC100.007077352145.09547145
GATCTCC151.2269337E-4145.09547145
GGCACCC256.445058E-6116.076385145
CTCGGCC301.5945616E-596.730316145
GACGACC301.5945616E-596.730316145
TGACGCC651.6370905E-1189.28953145
GGACCCC259.363367E-487.05729145
GAGAATA259.418315E-486.929159
AGACGCC451.2284399E-680.6086145
AATGGCC1000.079.80251145
CCTGTCC755.4569682E-1177.38426145
GGTCACC300.001930926772.54774145
GCCGACC607.99846E-872.54774145
GATAGCC502.2979293E-672.54774145
GCTGCCC851.6916601E-1068.28023145
GGTGCGT651.4068428E-766.8666156
GTGAATA651.4076977E-766.860713
GCACGCA1550.065.4228442
Produced by FastQC (version 0.11.5)