FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_1210_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_1210_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1711370
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG65310.38162407895428807No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT49930.2917545592127945No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA46780.27334825315390593No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG39410.2302833402478716No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT37110.21684381518900062No Hit
GTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCATAA31340.1831281371065287No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG31080.1816088864476998No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA30980.18102455927122715No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC30380.17751859621239124No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG29880.174596960330028No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA29180.17050667009471943No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT29110.17009764107118858No Hit
CTGTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCAT28860.16863682313000694No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG25870.1511654405534747No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG25250.14754261205934427No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA24800.14491313976521733No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG24560.143510754541683No Hit
GAATAAAGACTCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT24520.1432770236710939No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA24190.14134874398873418No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCATAAATAGGAGTA22440.1311230184004628No Hit
CAATTGGACCGATTACCCTTCAACACCAGAGAGGTGCCTTGATGACATAG21870.12779235349456866No Hit
GGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTT21080.12317616880043473No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT20950.1224165434710203No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC20880.12200751444748945No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA20190.11797565692982816No Hit
GAGCCATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAA19840.11593051181217387No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC19210.11224925060039617No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT19190.11213238516510164No Hit
GTTGTATTCAGTGCCGTTGCAGCAGGTTGGTTTCTGCATACTGTCCAATC18980.11090529809450907No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAGAGAAAGCTGAA18880.11032097091803643No Hit
GTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTCCATTTAC18640.10891858569450207No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA18600.10868485482391302No Hit
GTTTATTATTCCTTCATTGGAAAGCAGGACTGCGAGTTCTATTTGTGAGC18110.10582165165919702No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT17980.10506202632978258No Hit
ATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTC17980.10506202632978258No Hit
CCTTAATACTATTTCAGTTGGGCCATCAACATCCTCTGGTTCTTCCTCTA17660.10319217936507008No Hit
TCTTCATCCTCCACTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAG17470.10208195772977205No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC17310.10114703424741582No Hit
GTCCGGGAGCAACCAATGCCACCATAAACTTTGAAGCAGGAATTCTGGAG17280.10097173609447403No Hit
CATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCCACTGATGAT17140.10015367804741229No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGCC100.007076566145.1009145
GGTACCC306.475602E-10145.1009145
CGCACCC650.0111.61607145
GCCGACC301.5942687E-596.73393145
GAACGCC502.2586391E-887.06055145
AGTAGCC608.258212E-1084.64219145
CGACGAA1100.079.14595145
AGACGCC554.3752152E-879.14595145
AATGGCC1250.075.45247145
GTCGCCC300.001930642672.55045145
GCTGCCC702.7994247E-972.550446145
CACGCAC1550.070.097293
GCACGCA1550.070.097292
ACGTGCA1250.069.5365142
AGCACGC1600.067.906751
AACGCAG902.8558134E-1064.385665
GTGAATA1350.064.385663
TGACGCC808.047209E-963.481644145
GGCACCC350.003557120462.1861145
TCAGTCC350.003557120462.1861145
Produced by FastQC (version 0.11.5)