FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_1208_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_1208_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213149
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAAAATATCCCAGGAATTAATTAGAGTTTTTTTCGTCCAATGTCTCT9160.4297463276862664No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT8790.41238757864217046No Hit
GTAGTAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCAC7490.3513973792980497No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT7300.34248342708621665No Hit
GTTTTATCGTATGTCCCAGTCCAATATTTGCACTCTTAGCTGCAAAACTT6350.2979136660270515No Hit
ATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATGTTAG6290.2950987337496306No Hit
GTCCAATATTTGCACTCTTAGCTGCAAAACTTGATGATGCATCAAGGAAA6180.2899380245743588No Hit
GTTTAGTCTAATGTGAAAGCCCACGGCTTAACCGTGGAACGGCATTGGAA6130.287592247676508No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA5830.27351758628940315No Hit
ATTTAATGCATATGGATAAAATATCCCAGGAATTAATTAGAGTTTTTTTC5390.25287474958831613No Hit
GCTTAATACGTTAGCTCCAGCACCGAGGGCCGGAAAGCCCCCAACACTTA5030.23598515592379038No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT4720.22144133915711545No Hit
ACAACAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTC4720.22144133915711545No Hit
AGTAGAAACAACGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTG4720.22144133915711545No Hit
ATATATGCCAATAAGAAAACATTCTTTGCAAAAGTCTTCCATGCAAATAA4680.2195647176388348No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA4380.20549005625173003No Hit
GTAATACGTAGGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAG4090.19188455024419537No Hit
CTCCTGTGCTAAATGAAAAAAAAAAGAAAAAGAAAATATGACCCTACCTA4070.19094623948505504No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGT3980.18672384106892362No Hit
ATTAAAAGGGTGTGGCAGGTTAAGTCCACCACTCCTGTGCTAAATGAAAA3850.18062482113451153No Hit
ACCTAAAAATGAACTGCACTTTTTGTTGACATCAACAGCGTCAGGGTGGA3840.18015566575494138No Hit
GGATATTTAATGCATATGGATAAAATATCCCAGGAATTAATTAGAGTTTT3840.18015566575494138No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCC3810.1787481996162309No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGA3800.17827904423666074No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT3800.17827904423666074No Hit
GTTTATTGTCTCTTTGTTTGGACCTTTTATTCGTATTTTCATGTTTATTC3680.1726491796818188No Hit
CACGTGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCA3670.17218002430224866No Hit
ACAACAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCT3650.17124171354310833No Hit
CTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAACTACGTG3620.16983424740439787No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA3570.16748847050654705No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC3560.1670193151269769No Hit
GGCATATATTGTCTTCATGTGTTTCTAATGAAACAGAAGCCAGTGAGTCT3490.16373522746998578No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCAGGCACTGAAG3330.1562287413968632No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT3300.15482127525815276No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAGATAAAAGAACAAATT3260.1529446537398721No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC3120.14637647842588988No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA3040.14262323538932858No Hit
ATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG3020.14168492463018828No Hit
GTATGTCCCAGTCCAATATTTGCACTCTTAGCTGCAAAACTTGATGATGC2870.1346475939366359No Hit
CTCCAATGTTGCTTTGCAACTCTTCTGCCAGCTTTTGGACGTCTTCTCCT2830.13277097241835523No Hit
AAATATCCCAGGAATTAATTAGAGTTTTTTTCGTCCAATGTCTCTCGACT2800.13136350627964477No Hit
ATATTGTCTTCATGTGTTTCTAATGAAACAGAAGCCAGTGAGTCTCTGGG2790.1308943509000746No Hit
AATTAAAAGGGTGTGGCAGGTTAAGTCCACCACTCCTGTGCTAAATGAAA2740.1285485740022238No Hit
CACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTG2720.12761026324308347No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATC2680.12573364172480284No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA2650.12432617558609237No Hit
TTCAATACCTCCATGTTGTCAGAGAGCACTTCCTTCATTGTTTCTTTGGC2600.12198039868824156No Hit
GAATTAATTAGAGTTTTTTTCGTCCAATGTCTCTCGACTGTTTTATCGTA2580.12104208792910125No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT2570.12057293254953108No Hit
CTTTTGACTTGCCCCAAGAGATCTCAGCACTCCAATGTTGCTTTGCAACT2570.12057293254953108No Hit
GGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTG2530.11869631103125045No Hit
CCCATAATCTAGACATATTTTATTAAATTAAAAGGGTGTGGCAGGTTAAG2470.11588137875382948No Hit
GTATCTAACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGGTAAT2450.11494306799468916No Hit
AGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAAC2430.11400475723554884No Hit
ATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGACTTGCCCCAAGAGA2410.11306644647640852No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT2410.11306644647640852No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT2390.11212813571726821No Hit
GTCTAATGTGAAAGCCCACGGCTTAACCGTGGAACGGCATTGGAAACTGA2390.11212813571726821No Hit
GGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTT2390.11212813571726821No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAGATAAAAGAACAAATTGTAAG2330.10931320343984725No Hit
CCATAATCTAGACATATTTTATTAAATTAAAAGGGTGTGGCAGGTTAAGT2320.10884404806027709No Hit
GCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGTGAGACGAGAAAT2280.10696742654199645No Hit
GTAGTAACAAGAGCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC2280.10696742654199645No Hit
GATTTATTGGGCGTAAAGGGAGCGCAGGCGGTCTGTTTAGTCTAATGTGA2260.10602911578285613No Hit
ATATGACCCTACCTAAAAATGAACTGCACTTTTTGTTGACATCAACAGCG2260.10602911578285613No Hit
CTCCAGCACCGAGGGCCGGAAAGCCCCCAACACTTAGCACTCATCGTTTA2230.10462164964414565No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT2230.10462164964414565No Hit
GTGCTTTATGCGAGAAACAAGCATCACATTCACACAGGGCTCATAGCAGA2210.10368333888500533No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGA2160.10133756198715453No Hit
ATCTAGACATATTTTATTAAATTAAAAGGGTGTGGCAGGTTAAGTCCACC2150.10086840660758437No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGACC151.222363E-4145.18698145
CCCCGGG100.0070597124145.18698145
CACGCAC750.096.564213
GAGCATC259.416126E-486.907794
GTAACAA3700.082.210074
TAGTAAC3950.080.6739042
AGCACGC950.076.234911
TGGACGA1000.072.59348145
GCACGCA1000.072.423162
CAGCCTA851.70985E-1068.162975
TAACAAG4600.067.6999055
ACGTGCT1100.065.839242
AACACGT1100.065.839246
ACGTGCA451.1979817E-464.3761442
AGTAACA5100.062.4827273
GTGCTAG1200.060.3526344
AAACACG3050.059.3632475
CACGTGC2200.059.2553181
GTAGAAA5200.058.495631
ACACGTG1251.8189894E-1257.9385227
Produced by FastQC (version 0.11.5)