FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_1208_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_1208_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524973
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAAAATATCCCAGGAATTAATTAGAGTTTTTTTCGTCCAATGTCTCT63200.41443356702053086No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT58240.38190840100119805No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT50510.33121897895897173No Hit
GTAGTAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCAC50410.3305632296440658No Hit
ATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATGTTAG45550.2986938129396389No Hit
GTTTTATCGTATGTCCCAGTCCAATATTTGCACTCTTAGCTGCAAAACTT44790.29371011814635406No Hit
GTTTAGTCTAATGTGAAAGCCCACGGCTTAACCGTGGAACGGCATTGGAA43010.282037780341029No Hit
GTCCAATATTTGCACTCTTAGCTGCAAAACTTGATGATGCATCAAGGAAA40950.26852934445396737No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA40580.26610307198881555No Hit
ATTTAATGCATATGGATAAAATATCCCAGGAATTAATTAGAGTTTTTTTC38860.254824183772434No Hit
GCTTAATACGTTAGCTCCAGCACCGAGGGCCGGAAAGCCCCCAACACTTA35770.2345615299418416No Hit
ATATATGCCAATAAGAAAACATTCTTTGCAAAAGTCTTCCATGCAAATAA33610.2203973447398741No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT33240.21797107227472223No Hit
ACAACAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTC33220.21783992241174105No Hit
AGTAGAAACAACGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTG32730.21462675076870213No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA31730.20806925761964312No Hit
CTCCTGTGCTAAATGAAAAAAAAAAGAAAAAGAAAATATGACCCTACCTA30010.19679036940326156No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGT29510.19351162282873205No Hit
GTAATACGTAGGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAG28520.1870197046111636No Hit
ATTAAAAGGGTGTGGCAGGTTAAGTCCACCACTCCTGTGCTAAATGAAAA28330.18577378091284238No Hit
ACCTAAAAATGAACTGCACTTTTTGTTGACATCAACAGCGTCAGGGTGGA27700.1816425602289352No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA27230.17856053844887745No Hit
GGCATATATTGTCTTCATGTGTTTCTAATGAAACAGAAGCCAGTGAGTCT26990.17698674009310328No Hit
GGATATTTAATGCATATGGATAAAATATCCCAGGAATTAATTAGAGTTTT26870.1761998409152162No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGA25920.16997022242361012No Hit
GTTTATTGTCTCTTTGTTTGGACCTTTTATTCGTATTTTCATGTTTATTC25880.16970792269764776No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCC25870.16964234776615716No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC25830.16938004804019482No Hit
CTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAACTACGTG25600.1678718246159112No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT25470.16701935050653358No Hit
ACAACAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCT24720.16210123064473927No Hit
CACGTGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCA24030.15757656037188855No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA23890.1566585113310203No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCAGGCACTGAAG23040.1510846421543201No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT22610.14826492010022474No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAGATAAAAGAACAAATT21310.139740179006448No Hit
ATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG20990.1376417811987491No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC20590.13501878393912548No Hit
ATATTGTCTTCATGTGTTTCTAATGAAACAGAAGCCAGTGAGTCTCTGGG20120.13193676215906772No Hit
CTCCAATGTTGCTTTGCAACTCTTCTGCCAGCTTTTGGACGTCTTCTCCT19690.12911704010497235No Hit
GTATGTCCCAGTCCAATATTTGCACTCTTAGCTGCAAAACTTGATGATGC19680.12905146517348176No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA19390.12714979216025463No Hit
AAATATCCCAGGAATTAATTAGAGTTTTTTTCGTCCAATGTCTCTCGACT19020.1247235196951028No Hit
GGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTG18760.12301857147634745No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATC18730.12282184668187567No Hit
AATTAAAAGGGTGTGGCAGGTTAAGTCCACCACTCCTGTGCTAAATGAAA18730.12282184668187567No Hit
TTCAATACCTCCATGTTGTCAGAGAGCACTTCCTTCATTGTTTCTTTGGC18320.12013327449076149No Hit
CACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTG18270.11980539983330851No Hit
GAATTAATTAGAGTTTTTTTCGTCCAATGTCTCTCGACTGTTTTATCGTA18260.11973982490181792No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT17980.11790372682008139No Hit
GCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGTGAGACGAGAAAT17840.11698567777921315No Hit
CCATAATCTAGACATATTTTATTAAATTAAAAGGGTGTGGCAGGTTAAGT17460.1144938303825707No Hit
GTATCTAACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGGTAAT17380.11396923093064598No Hit
ATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGACTTGCCCCAAGAGA17380.11396923093064598No Hit
AGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAAC16780.11003473504121056No Hit
GGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTT16700.10951013558928584No Hit
GTAGTAACAAGAGCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC16630.10905111106885171No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT16550.10852651161692699No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT16290.10682156339817164No Hit
ATATGACCCTACCTAAAAATGAACTGCACTTTTTGTTGACATCAACAGCG16220.1063625388777375No Hit
CTTTTGACTTGCCCCAAGAGATCTCAGCACTCCAATGTTGCTTTGCAACT16120.1057067895628316No Hit
CCCATAATCTAGACATATTTTATTAAATTAAAAGGGTGTGGCAGGTTAAG16010.10498546531643511No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAGATAAAAGAACAAATTGTAAG15980.10478874052196334No Hit
GATTTATTGGGCGTAAAGGGAGCGCAGGCGGTCTGTTTAGTCTAATGTGA15890.10419856613854803No Hit
CTCCAGCACCGAGGGCCGGAAAGCCCCCAACACTTAGCACTCATCGTTTA15830.10380511654960449No Hit
GTCTAATGTGAAAGCCCACGGCTTAACCGTGGAACGGCATTGGAAACTGA15770.10341166696066094No Hit
ATCTAGACATATTTTATTAAATTAAAAGGGTGTGGCAGGTTAAGTCCACC15670.10275591764575503No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT15480.10150999394743382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG450.0128.99759145
AACGGCC550.0118.73643145
CACGCAC5350.0112.3611153
AGGCGCC650.0111.632545145
GTAACAA27800.094.052074
TAGTAAC30050.087.489092
TAACAAG34350.075.911965
ACCCGCC300.001929473572.56114145
AGTAACA38000.069.3760453
ACGTGCA3400.068.1652152
GCACGCA8850.067.924522
AACACGT9950.067.711996
GTGGTCC451.18908705E-464.498795145
GGTGCGT451.2001459E-464.378253
CAGCTGG8300.063.705845
ACACGTG10700.062.2887657
AGACGGG350.003554968262.19527145
CCACGGG350.003554968262.19527145
CAACGCA11200.061.456569
GTAGTAA44700.060.9217071
Produced by FastQC (version 0.11.5)