FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14462406
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAC7400.076.610453
CGCACTT9450.062.9884265
ACGCACT10800.057.0854264
ACACGAG8850.049.6302037
TAGTAAC25000.049.0383532
GCACGCA12350.045.904242
TAACAAG27550.043.9832955
AAACACG12700.043.5215265
CACGAGC10850.040.4817778
AGGCGCC1405.619713E-737.014767145
CACTGTG10800.036.7334637
CACGTGC7800.034.529311
CGGTGCG1352.5060597E-531.4954072
CCTCGCC950.004270168531.17033145
GGTGCGT950.005287548529.8377553
ACGAGCC4850.027.7529729
CCGGTCC2304.373942E-827.7212169
ACACGTG4150.027.3130077
AACAACG10400.027.247357
CGGAGCG1607.98437E-526.573245
Produced by FastQC (version 0.11.5)