FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_DEMUX_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_DEMUX_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGACCTGGGCGCTGAAGAACGGCATCAAGCGCGTAGTCACGCTGGT21.3513513513513513No Hit
CGTCAACACTAGGTAGATGTCAGGAGTGTATTTTCCAAGGAAAAGCATCA21.3513513513513513No Hit
GAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACA21.3513513513513513No Hit
GTCCGGGAGCAACCAATGCCACCATAAACTTCGAAGCAGGAATTCTGGAG21.3513513513513513No Hit
GCTCACACATTTTCTCATTTTTCTCTCAGCCAGAATCAGGCCCTTCTTTT21.3513513513513513No Hit
TAGTAGAAACGAATAAATGATTACAATGGAATTTCAGAGGCATTATTAAA21.3513513513513513No Hit
CGTATAGAATGCTAGCCTAGCAGTACCCTGAGTACCGCGGGGTCGGAGAC10.6756756756756757No Hit
CTGGGTGATGGGCGAGCAATTCACCATTGCCGACATCGCCCTGTGGCCCT10.6756756756756757No Hit
ACGTTGTCATGCTGGCCAGGAAGTGGGTTAACCCGTTAAACAGGGCAGCT10.6756756756756757No Hit
ACCTGAAACCGTGTGCTTACAAGCGGTCGGAGCTGGCAGGTCCAGTGACG10.6756756756756757No Hit
CTCACACATTTTCTCATTTTCTCTCAGCCAGAATCAGGCCCTTCTTTTTT10.6756756756756757No Hit
TCTCGTCTCACTCCAGGCACTGAAGATCTCGCTGCTCTGCTATGAGCCCT10.6756756756756757No Hit
GTCGTCCAAATCTTTGTCTTTGTCTGATCTTTAATCCTAATGCAGCTCTT10.6756756756756757No Hit
GTCTGTCTCCCCTGGGTTAATTTGTCTACAGTTGTGACCATTAGTGCCTC10.6756756756756757No Hit
CCTTCTCTTCCAGGACATACTTATGAGGATGTCAAAAATGCAGTTGGGGT10.6756756756756757No Hit
GACAAGAGTAACTCGTAGGCTCATTATGCAAAAGGCACGCCGTCACTGGA10.6756756756756757No Hit
GTCCTCCATTACATGTTACTGCAACTTCAACCTGCCCAAGGGTAGATCAC10.6756756756756757No Hit
CTCTGAGAAGATTGGGTAGTTGCCTGATTTTTGTTCTGTCGTGCCTTATA10.6756756756756757No Hit
GTAATAAAAAGTACTGGAATATTAACCAGTTGTCCATCGGCTTACTCCCT10.6756756756756757No Hit
GCGGACGCGGTGCTGCCGCGTGGTGGCTGGACGCCGTGTGCCGCCGCCAC10.6756756756756757No Hit
TGCATCTCGCGGGCAATCGCATCCCGCTCCTGTTGGCGGGTGCGCTCACC10.6756756756756757No Hit
CCTCGTTAGTTCAACTTAGCGTATATTTAGGGACCTTAGCTGATGGTCTG10.6756756756756757No Hit
AGATAGATCAATACAAATTATCCAACTTGAATAACATGTAGAGAATTACC10.6756756756756757No Hit
GATAGATACTACAACTATTCTCATTTTATAGCTATGGAAATTGAAACCCA10.6756756756756757No Hit
CGTCGAAGGTGTCTCCGCCCTGCTGCAGCCAGTGGAAGGCATCGGTGTTG10.6756756756756757No Hit
TAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGGATCTC10.6756756756756757No Hit
GTATTATTAGAGCATAAAGTACATGACTTGAGGCTGGAGAAGTAGGTTCC10.6756756756756757No Hit
CTGGGTTAGTACAGGAAAGGTACATGAATGTGAGGAATAACCAGAGAGTT10.6756756756756757No Hit
CTACTATATTATTACCATTATTTTAATATATCATAGAACCCATTGTAAAA10.6756756756756757No Hit
ACAACAGCACCTTAGGATTCTCTCCTCGACTACCTGTGTCGGTTTACGGT10.6756756756756757No Hit
GGGGCAATGGCAGCAGGTGGGCGTGTTTCCGCGCGGCTCGGGTATCAACG10.6756756756756757No Hit
CTTATTGAAGTGAAAATATTTATCACCTTACAGAACAACTTAAATTCTTT10.6756756756756757No Hit
CTGATAAGATAAAAGAACAAATTGTAAGAATCTGAAATGGTTCGAGCATT10.6756756756756757No Hit
GGTAATGGGCCGTGGAGTGGATAGAAAAGCAAATGTAGGCATAAGTAACG10.6756756756756757No Hit
AGTATATGCTCCGGAAGCATATATCTAGCCCCCGACGGGAAATCCTCGGT10.6756756756756757No Hit
TGCCCAGTTGGAGGGATTCAGTGCCGAGTCTAGGAGACTTCTACTGTTGA10.6756756756756757No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAACTGTGT10.6756756756756757No Hit
GGGGGACACTGATGTTGTAACAGTTGTAACTTTCGAATTTAGTAGTACAG10.6756756756756757No Hit
CGTATAGAATGCTAACCTAGCAGTACCCTGAGTACCGCGGGGTCGGAGAC10.6756756756756757No Hit
ATTCAATGCAAGTAAAACTAGGAACACTCTGTGCTTTGTGCGAAAAACAA10.6756756756756757No Hit
CCTGCGTAACGTCATCGGTTCGATGGACCTGGAGCAGACCTTGACCAGCC10.6756756756756757No Hit
GATCTGGATGTGGCCTTGGGCAGGCCAATGTGTGTGGGGACCACACCTTC10.6756756756756757No Hit
GCCACAGCTTGCTCCAACTCCTCCTTCCGCTGCTGATAGCGAGCGGTCAG10.6756756756756757No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC10.6756756756756757No Hit
CCTGCGCGCCGTGGCCTCCGATCCGCCGCTGGATCTGTGGAGCTGAGGCT10.6756756756756757No Hit
GGCCTGATAGGGGTTCTCTGCCCATTGCTGCGGCAGGAAGAGATGGCCTG10.6756756756756757No Hit
GTAGCAGATGGTGGGCCCAACATTTACAATTTGAGAAACTTACATATCCC10.6756756756756757No Hit
GGTGCCCGACGCCGAGGTGCGGACCGGTCCGCGGGTGGGCGTGGCCGCCG10.6756756756756757No Hit
CTTCCGTAGAAGGCCCTCTTTTCAAACCGTATTTAAAGCGACGATAAATG10.6756756756756757No Hit
CACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATAAGCATGAACCAGT10.6756756756756757No Hit
ATTCTGAGCCACGTTCGAAAACGGTGGTTTTCGAGCAGCTGGTAAGTATT10.6756756756756757No Hit
GAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGGATCTCGCTGC10.6756756756756757No Hit
GCAGGCTGGTTTCTGCATACTGTCCAATCGAAAGTCTGTCTCCCCTGGGT10.6756756756756757No Hit
GGCCCGCTGAGCACCAGGTAGGCCGCACGAGGTGGCTGGTAACAGCCATC10.6756756756756757No Hit
GGATTGAAAAGAATCCTTCACTAAGGATGAAGTGGGCCATGTGTTCTAAC10.6756756756756757No Hit
ATAGAATGCTAGCCTAGCAGTACCCTGAGTACCGCGGGGTCGGAGACGCC10.6756756756756757No Hit
GCATTTAGTAAATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTA10.6756756756756757No Hit
GTCACTGGACCTGCCAGCTCCGACCGCTTGTAAGCACACGGTTTCAGGTT10.6756756756756757No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG10.6756756756756757No Hit
GAATCTGGAATGGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAAT10.6756756756756757No Hit
GCCATAATCAGGCCCTTCTTTTTTGTTGCTGTTGTTCCCATTCCCGATAA10.6756756756756757No Hit
GGCCGCACAGGCGCTGCGCTCGCCTGACGCCAGCCATTCGGCCGCCATGC10.6756756756756757No Hit
GGTCTGTAGTTTGTCTTTAGGTTGTTGGTGAATGTGAGGACTCAGGTGAT10.6756756756756757No Hit
ATTGTCAGTTGTGTGTTCCAAGCCGATCTTTCGCAGTCCTCGTTCTCTCT10.6756756756756757No Hit
GTGCCAATAGATGCTCGTCTTCACTGTTTATTATTCCTTCGTTGGAAAGC10.6756756756756757No Hit
CATTAGTTCATTTATCCCATGTAGCTCACTTGCTTTGGGTGATTCCTCAT10.6756756756756757No Hit
GAATACTAACATCAGAATCACAGCTAACAATAACTAAAGAGAAAAAAGAA10.6756756756756757No Hit
TTCTTAATCTATGGGCCATAGCCTCAAGCATGCTATGTTGCCCTACTGGT10.6756756756756757No Hit
GACCAAGGGGGACATGGCAAAAAGAATCACCAAGGAATACAAGGGAATAC10.6756756756756757No Hit
CCACTTAACCTTGCCAGACAAGAGTAACTCGTAGGCTCATTATGCAAAAG10.6756756756756757No Hit
ACTGTAAGTATACTGATCAAATTGCGTTTCTTGAGTTGTATGATCGACTG10.6756756756756757No Hit
CATCTAGCCCTCTCACCATCACGGTCAAGCCGGGCGCACCATGCGTGCGA10.6756756756756757No Hit
GAAGAGGCAACACCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGG10.6756756756756757No Hit
TGAAGGATGCGCTGGCAGCGGCGGGCGACGTCCTCATCATGGGTGACGAC10.6756756756756757No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCAT10.6756756756756757No Hit
GACTAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGAACTAGAT10.6756756756756757No Hit
ATTGAAAATATTCGAATCGAAAAGAATCGAAAAGACTCGATTCAAATCGA10.6756756756756757No Hit
ATGTCAAGGAGTTTGGACTTCATCCTAGGAGCTGCAGAGCGGTTTTGAGC10.6756756756756757No Hit
GGCCTCTAGATTGAATAGACGGGACATTCCTCAATCCTGTGGCCAGTCTC10.6756756756756757No Hit
GTTATAAAAAGTACTGGAATATTAACCAGTTGTCCATCGGCTTACTCCCT10.6756756756756757No Hit
GCGTCGCCCTCCGCAACATCCATAGCGTCGCCCTCGTTTCCCACTGCTTC10.6756756756756757No Hit
GAGGATCCGATGGCCATCTTCTTCATCCTCCACTGTAAGATCATCAGTAG10.6756756756756757No Hit
CAGCTCCATTCAAATCATTCAATCTGAAGGAGGTTATTGTTGTGTTTAGT10.6756756756756757No Hit
AAAAAAGAGGCTGAGCATGGTTGCTCGTGCCTATAATCCCAGCCCTTTGG10.6756756756756757No Hit
GCTACTCAGGAGGCTGAGGCGGGAGAATCACTTGAACCCGGGAGGTAGAG10.6756756756756757No Hit
GTCGTATAAGACGGATCTGCAGATAACACCAGGCCTTTTAGATGAATGAC10.6756756756756757No Hit
ATCCAACTATGATGACGGAAAATAGGGAAGCTCTATAGGATTCGATCCTC10.6756756756756757No Hit
CAGCGAGGCATTGCGCTGCGGCGAGGTCACGGCCACGGCGCGCACCTTGC10.6756756756756757No Hit
GCCTTATCGTTACTTATGCCTACATTTGCTTTTCTATCTACTCCACGGCC10.6756756756756757No Hit
ATGTAACGGGGCTCAAGCACGCCACCGAAGTTGTGGCATTGACATTTTTG10.6756756756756757No Hit
GCTTAATCTGGGGAGAAATAACATCCCTTTCAGACAAGCAAATTCCGAGT10.6756756756756757No Hit
ATACAGTTGGATTGATTACCCTTCAACCCCAGGGAGGTGCCTTGATGACA10.6756756756756757No Hit
GAGCTGATAAGATAAAAGAACAAATTGTAAGAATCTGAAATGGTTCGAGC10.6756756756756757No Hit
CTAGTTGATAGGGCAACTAGGATAGCAAAGTTCTAGTGTCCAGAGCAGGC10.6756756756756757No Hit
TGCTGGTCAAGCGACGGGGCATCGGCATGTTCGTCGAACCCGGGGCGCGG10.6756756756756757No Hit
TCACAAGGTTTCGCGTCTACCTCATCTGACTATGCGCCCTATTAAGACTC10.6756756756756757No Hit
CACAATAACACAGGAGTCCCACACATATGAGATTCGACGAAGGGCCAGAT10.6756756756756757No Hit
GTAGTAACAAGAGCTATTTCCCAGTTTGATTAGCCTTTCACCCCTACCCT10.6756756756756757No Hit
GGTATAGGGACGGTAGGCAAATCCGCCGACCCTGCTGAAACCCAATAGTA10.6756756756756757No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG10.6756756756756757No Hit
GTGGGAATCTGTCAGCACCATCTGATAAGGCTAAATACTCCTGAGAGACC10.6756756756756757No Hit
GGCAGACGTCGGGGGAATAATTCAAAGACACAGCGGCGGTTGGCAGTCCC10.6756756756756757No Hit
GTCCTCTCGTACTAGGAGCAGCCCCAATCAATCATCCAACGCCCACGGCA10.6756756756756757No Hit
CGGTAGCTCTACCTCTATAAGACTCCATGCCGACGCTGTTCCTAAAAACA10.6756756756756757No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT10.6756756756756757No Hit
GGCCGGCCTCCAGCGCGTCGATGGCCAGCAGGTAGCGCATGCGCTGGCGC10.6756756756756757No Hit
ATTCTTATGAGGTAGCGAGTGTTGCTGTGGAGCCCAGCGCCATTGACGAC10.6756756756756757No Hit
GTCGGAGACGCCCTGTGGGAATCTGTCAGCACCATCTGATAAGGCTAAAT10.6756756756756757No Hit
GGATCCTTTTACTAGTTTTCCTTCTTGAGTTCCAATACTGAAAGTTTTGG10.6756756756756757No Hit
CCTTTCACCCCTACCCTCAGGTCATCCGGAAACTTTTCAACGTTTATCGG10.6756756756756757No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGGTTTGT10.6756756756756757No Hit
TTTTTCTCTCAGCCAGAATCAGGCCCTTCTTTTTTGTTGCTGTTGTTCCC10.6756756756756757No Hit
GCTTTGGTCATGTCGTATCCTTGTATCGAGGATTCTTGTTCAACAATTGT10.6756756756756757No Hit
TTGTTATGCAGGGCATGATTGTATTTAAATTTGGTACCTGTGAACAAAAA10.6756756756756757No Hit
GTCATCTAGCGGCCGAACGGGTGGCTGCTCCCGTTTCATCGCTTGTGCGA10.6756756756756757No Hit
TTCATGGCCTTCTGCTATTTCAAATGCTTCATGAAAGCTCACACATTTTC10.6756756756756757No Hit
CATTTATGTTCCTCTCTAAATGGGTTATTTTAGTTAGCAGCTCCTGTAAC10.6756756756756757No Hit
CTCCTGAGAGACCGATAGTGAACTAGTACCGTGAGGGAAAGGTGAAAAGA10.6756756756756757No Hit
CTTCAATCCAATCAAGTTGACACTCAATATTAACCATCACAAGCTGATAA10.6756756756756757No Hit
GTTTAGGGAATAACAACATGAAATAAGTCTGTACATGTTCACTACAGATG10.6756756756756757No Hit
CCAGCCAGCAGACCGTCACGATCCGCCGCAACGCCGACGAAGTCGCCTTG10.6756756756756757No Hit
GCCATATTGTAAAGAGCCATGGCTTCGTACCCAACCATAGAGTACTCTTC10.6756756756756757No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA10.6756756756756757No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT10.6756756756756757No Hit
GTGGTTCAGCCACGTATCCGAAGCGAAAGCGAGTCTTAATAGGGCGCATA10.6756756756756757No Hit
GGCTTTGGTCATGTCATATCCTTGTATTGATGATTCCTGTTCAACAATTG10.6756756756756757No Hit
GAAAAGAACCTCGAACAGAGGAGTGAAAAGAACCTGAAACCGTGTGCTTA10.6756756756756757No Hit
CTGTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTAC10.6756756756756757No Hit
ATTAAAGGAAAGGGGTTTCTTCGGAGCTATTGCTGGTTTCTTAGAGGGAG10.6756756756756757No Hit
CATTTTGTAGGTTGCCTGTTCGTTCTGATGGTAGTTTCTTTTGCTGTGCA10.6756756756756757No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT10.6756756756756757No Hit
GGTTCAGTATCTATCACGGTCTTATTTGCAATTTCTACAGCTAGCTCCAA10.6756756756756757No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA10.6756756756756757No Hit
GTTCCTAAAAACATTTCGGGGAGTACGAGCTATTTCCCAGTTTGATTAGC10.6756756756756757No Hit
ATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC10.6756756756756757No Hit
AATAGTGATAGGAAATTGCCAACTCTAACAGTAAACTTTTGGGAATTTTT10.6756756756756757No Hit
CAATGGTCCAACGACGGCAGTCGAAAGCCCACGTTCGTGTCCGTCACACG10.6756756756756757No Hit
CCTTAGGATTCTCTCCTCGACTACCTGTGTCGGTTTACGGTACGGGTTTT10.6756756756756757No Hit
AGCTCACACATTTTCTCATTTTTCTCTCAGCCAGAATCAGGCCCTTCTTT10.6756756756756757No Hit
ATTCGTATTTTCATGTTTATTCCTCTTTTTATTTGATTCAAATGCCCTAT10.6756756756756757No Hit
ACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAAT10.6756756756756757No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT10.6756756756756757No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers