FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_9974_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9974_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1891839
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG93700.49528527533262606No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT84050.4442767064216352No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA78410.414464444384538No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCTGATGGATTCAT66630.352196989278686No Hit
GTGTTAAGTCATCCATGCTTTGACATTTTTCAGGAAACCTTCTATCTTAG62450.3301020858540288No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT60740.32106326172576No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT56370.2979640445090729No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA54550.2883437755538394No Hit
GGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGTCAAGAATAAA51880.2742305238447881No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT49210.2601172721357367No Hit
GTATGAAAAGTTCCTCAGAGAGCAACTGTCCGAAATACAGTTGGATTGAT48540.2565757445533156No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC47370.2503912859392369No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA45800.2420924824998322No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC44610.2358023066444872No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT44120.2332122342334628No Hit
CAATATGACCACAACACAAATTGAGGTGGGTCCGGGAGCAACCAATGCCA43900.23204934457953347No Hit
ATCAAAGGCTGTGCCCTATCCTGGCTTAGGTCTTACCTCTCTGACCGACT42420.22422626872582707No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG41530.21952185148947662No Hit
TGACTACCCTGGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGT41240.21798895149111527No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG41070.2170903549403517No Hit
GTATGGCTCAAACCCTTCAATTCCAGCTGATGGATTCATAAATAGAAGTA38680.20445714460902856No Hit
GAGCCATACTGTATGAAAAGTTCCTCAGAGAGCAACTGTCCGAAATACAG37890.2002813135790096No Hit
GTAGTAACAAGAGCATCATCACTTCTGTTGGATGTTTGTGAGATGTCATC37110.19615834116962386No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG37080.1959997653077244No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT36170.19118963083010765No Hit
ATCCTGGCTTAGGTCTTACCTCTCTGACCGACTTCAATTTGTACATGTCC35830.1893924377285805No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA35750.18896956876351528No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTTCTA35010.18505803083666209No Hit
AGTAGAAACAACGCACTTTGGTTGTTGGTATCAAAGGCTGTGCCCTATCC34670.18326083773513496No Hit
ACATTACGCATATCCCTTCTTATTGTCAAACGGAACTTCCCTTCTTTCTG33610.17765782394802096No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA32620.172424820505339No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT32310.1707862032657113No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT32090.16962331361178198No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA30060.1588930136232523No Hit
CTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG29880.1579415584518556No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT28790.1521799688028421No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGTGATTCTTCTACCTCCAAA28720.15180995845841005No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG28380.1500127653568829No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT28320.149695613633084No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC28160.14884987570295358No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT27630.1460483688093966No Hit
TCTGTGACCAGTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTT27020.14282399295077436No Hit
CTCAGGGACAATACATTACGCATATCCCTTCTTATTGTCAAACGGAACTT26950.1424539826063423No Hit
GTATTGTCCCTGAGAGTATTAGTAAACGGAACATTCCTCAAACACCCCAA26730.14129109295241296No Hit
GTCCGGGAGCAACCAATGCCACCATAAACTTCGAAGCAGGAATTCTGGAG26530.14023392053974995No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTTGAAGAGAGAAAAG26450.13981105157468474No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT26100.13796099985252444No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC26000.13743241364619294No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTACTGTTTTGT25840.13658667571606253No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA25480.1346837653732691No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA25340.13394374468440495No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG25150.1329394308923751No Hit
CCTTAAGGCTCAGTGCCTGGGCCCCTGCTCTTCTCTTTGTACATGCTACC24180.12781214469095942No Hit
GTAAAAGGATGTCTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG23910.12638496193386436No Hit
ATCTTAGGTAGTTTAGGTTGGTATAATTTCATCAGGTTTCATGGATAGGT23870.12617352745133173No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT23650.12501063779740243No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA23110.12215627228321226No Hit
GAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACA22890.12099338262928294No Hit
ATTCTGGAGTGCTATGAAAGACTTTCATGGCAAAGGGCCCTTGACTACCC22340.11808615849445964No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT22220.1174518550468618No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG21950.11602467228976673No Hit
GCGTAATGTATTGTCCCTGAGAGTATTAGTAAACGGAACATTCCTCAAAC21920.11586609642786727No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT21800.11523179298026946No Hit
ACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA21600.11417462056760644No Hit
TACTAATACTCTCAGGGACAATACATTACGCATATCCCTTCTTATTGTCA21600.11417462056760644No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG21210.11211313436291354No Hit
CCTTAATACTATTTCAGTTGGGCCATCAACATCATCTGGTTCTTCTTCTA20980.11089738608835106No Hit
GAATAAAGACCCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTT20870.11031594126138641No Hit
CATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCTACTGATGAT20780.10984021367568804No Hit
CTTCTGTTGGATGTTTGTGAGATGTCATCGAGTGTGAGTAAATGGCTGGA20740.10962877919315545No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC20740.10962877919315545No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC20250.10703870678213104No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG20130.10640440333453323No Hit
TCTTCATCCTCCACTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAG20030.10587581712820171No Hit
GTCCCTGAGAGTATTAGTAAACGGAACATTCCTCAAACACCCCAATGGAT19990.10566438264566912No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT19990.10566438264566912No Hit
ATACATTACGCATATCCCTTCTTATTGTCAAACGGAACTTCCCTTCTTTC19640.10381433092350881No Hit
GGACAATACATTACGCATATCCCTTCTTATTGTCAAACGGAACTTCCCTT19610.10365575506160937No Hit
GCCCTATCCTGGCTTAGGTCTTACCTCTCTGACCGACTTCAATTTGTACA19530.10323288609654416No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA19220.10159426885691647No Hit
TCATTAAGACGCTCGAAGAGTGAATTGAGGATCCGATGGCCATCTTCTTC19080.10085424816805234No Hit
TTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTG18960.10021994472045453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACG253.697278E-8145.101091
GTACTAG100.007076599145.101071
AGCACGC11650.0115.831771
GCACGCA11800.0114.323052
CGGTGCA700.0113.9718252
CACGCAC11950.0113.494953
GCGGTGC1500.096.7340551
GGTGCAT850.093.8591463
ACGTGCA502.3018401E-672.527532
GGTGCGT406.6522014E-572.527523
CAGCGGG406.6548164E-572.5217745
TAGTAAC24450.065.556572
CGGTGCG451.1918914E-464.468922
CGCACTT23000.063.6930355
CACTTTC28350.063.420447
ACGCACT23150.063.2853554
ACAACGC9200.060.6783188
CAACGCA9700.057.550579
AAACACG2900.057.517275
GAAACAC3050.057.070844
Produced by FastQC (version 0.11.5)