FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_9974_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9974_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1903395
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG94290.49537799563411694No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT84500.44394358501519654No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA78870.4143648585816397No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCTGATGGATTCAT67010.3520551435724062No Hit
GTGTTAAGTCATCCATGCTTTGACATTTTTCAGGAAACCTTCTATCTTAG62800.3299367708751993No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT61030.3206375975559461No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT56770.2982565363468959No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA54740.2875913827660575No Hit
GGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGTCAAGAATAAA52160.2740366555549426No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT49500.2600616267248784No Hit
GTATGAAAAGTTCCTCAGAGAGCAACTGTCCGAAATACAGTTGGATTGAT48740.2560687613448601No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC47600.2500794632748326No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA46060.2419886571100586No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC44760.2351587558021325No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT44340.2329521723026487No Hit
CAATATGACCACAACACAAATTGAGGTGGGTCCGGGAGCAACCAATGCCA44170.23205903136238143No Hit
ATCAAAGGCTGTGCCCTATCCTGGCTTAGGTCTTACCTCTCTGACCGACT42640.22402076289997608No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG41680.2189771434725845No Hit
TGACTACCCTGGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGT41450.2177687763181053No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG41380.21740101240152465No Hit
GTATGGCTCAAACCCTTCAATTCCAGCTGATGGATTCATAAATAGAAGTA38840.2040564359998844No Hit
GAGCCATACTGTATGAAAAGTTCCTCAGAGAGCAACTGTCCGAAATACAG38110.2002211837269721No Hit
GTAGTAACAAGAGCATCATCACTTCTGTTGGATGTTTGTGAGATGTCATC37370.19633339375169107No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG37250.1957029413232671No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT36370.19107962351482483No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA36040.18934587933665897No Hit
ATCCTGGCTTAGGTCTTACCTCTCTGACCGACTTCAATTTGTACATGTCC36020.18924080393192164No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTTCTA35200.18493271233769132No Hit
AGTAGAAACAACGCACTTTGGTTGTTGGTATCAAAGGCTGTGCCCTATCC34840.1830413550524195No Hit
ACATTACGCATATCCCTTCTTATTGTCAAACGGAACTTCCCTTCTTTCTG33750.1773147454942353No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA32760.17211351295973773No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT32570.17111529661473315No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT32260.16948662784130464No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA30270.1590316250699408No Hit
CTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG30130.15829609723677954No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGTGATTCTTCTACCTCCAAA28910.15188649754780273No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT28890.15178142214306542No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG28570.15010021566726822No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT28440.1494172255364756No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC28310.148734235405683No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT27730.1456870486683006No Hit
TCTGTGACCAGTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTT27160.14269239963328684No Hit
CTCAGGGACAATACATTACGCATATCCCTTCTTATTGTCAAACGGAACTT27080.14227209801433754No Hit
GTATTGTCCCTGAGAGTATTAGTAAACGGAACATTCCTCAAACACCCCAA26820.1409061177527523No Hit
GTCCGGGAGCAACCAATGCCACCATAAACTTCGAAGCAGGAATTCTGGAG26720.1403807407290657No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTTGAAGAGAGAAAAG26600.13975028830064173No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT26330.13833177033668786No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC26150.13738609169405194No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTACTGTTTTGT25930.13623026224194137No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA25680.13491681968272481No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA25450.1337084525282456No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG25320.13302546239745297No Hit
CCTTAAGGCTCAGTGCCTGGGCCCCTGCTCTTCTCTTTGTACATGCTACC24270.12750900364874343No Hit
GTAAAAGGATGTCTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG24020.12619556108952687No Hit
ATCTTAGGTAGTTTAGGTTGGTATAATTTCATCAGGTTTCATGGATAGGT24020.12619556108952687No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT23790.12498719393504763No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA23180.12178239409055924No Hit
GAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACA23100.12136209247160994No Hit
ATTCTGGAGTGCTATGAAAGACTTTCATGGCAAAGGGCCCTTGACTACCC22440.11789460411527823No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT22370.1175268401986976No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG22110.11616085993711237No Hit
GCGTAATGTATTGTCCCTGAGAGTATTAGTAAACGGAACATTCCTCAAAC22060.11589817142526905No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT21930.11521518129447646No Hit
TACTAATACTCTCAGGGACAATACATTACGCATATCCCTTCTTATTGTCA21690.11395427643762855No Hit
ACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA21660.11379666333052256No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG21330.11206291915235671No Hit
CCTTAATACTATTTCAGTTGGGCCATCAACATCATCTGGTTCTTCTTCTA21050.11059186348603416No Hit
GAATAAAGACCCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTT20980.11022409956945353No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC20850.10954110943866091No Hit
CATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCTACTGATGAT20830.10943603403392359No Hit
CTTCTGTTGGATGTTTGTGAGATGTCATCGAGTGTGAGTAAATGGCTGGA20820.10938349633155495No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC20310.10670407351075314No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG20190.1060736210823292No Hit
TCTTCATCCTCCACTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAG20100.10560078176101124No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT20080.10549570635627394No Hit
GTCCCTGAGAGTATTAGTAAACGGAACATTCCTCAAACACCCCAATGGAT20040.10528555554679926No Hit
ATACATTACGCATATCCCTTCTTATTGTCAAACGGAACTTCCCTTCTTTC19730.10365688677337075No Hit
GGACAATACATTACGCATATCCCTTCTTATTGTCAAACGGAACTTCCCTT19720.10360434907100208No Hit
GCCCTATCCTGGCTTAGGTCTTACCTCTCTGACCGACTTCAATTTGTACA19630.10313150974968412No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA19310.10145030327388693No Hit
TCATTAAGACGCTCGAAGAGTGAATTGAGGATCCGATGGCCATCTTCTTC19200.10087238854783163No Hit
TTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTG19060.10013686071467037No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAAG100.00708655145.032933
CCCTGCC253.712921E-8144.99864145
CGTGCTC256.4666892E-6115.99891145
AGCACGC11500.0105.942661
GCACGCA11500.0105.9370962
CACGCAC11550.0105.478493
CAGCGGG301.5982381E-596.686085
GCGTTTT353.43555E-582.869426
ACGTGCA801.8189894E-1281.581022
GAAACAC3150.080.573854
AAACACG3150.080.571735
GGTGCAT554.3895852E-879.108873
ACAACGC11050.074.795678
CGGTGCA608.0230166E-872.5164642
CAACGCA11450.072.1827249
GCGGTGC1350.069.8343351
AAACAAC12600.067.331416
AACACGA2800.067.3314066
ACACGTG1009.094947E-1265.249397
ACACGAG2900.064.999397
Produced by FastQC (version 0.11.5)