FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_9932_2_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9932_2_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1857783
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG86780.46711591181531964No Hit
GTATAAATACTGGGACAATTGACAAAACACCGGAAGAAATAACTTCTGGA69340.37324057761320883No Hit
TCATAATCCTCTGCTGTGTCCCTCCCAAAGAAGAAATTGGGGATGGTCTG52980.2851786241988435No Hit
GTATTTATACCGTCAATATCCATGTTGGACATTTTGATTTTCAGTTCTTT51270.27597410461824656No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT43210.23258905910970226No Hit
CAGTTATAGTGTCTATTTTGTTGCTTTAATAATCGAGGTCATCATAATCC42600.22930557551662387No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT37160.20002336117835076No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT35080.18882722040195224No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA32490.17488587203134057No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC31090.16735000804722616No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG30030.16164428245925386No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT30000.16148279965959425No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG28620.15405459087525294No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT27650.14883331368625938No Hit
TTCTTATAGGGCTGAAGTAGATGGTCTTATCATCTCTTACCATCTTGTAA27240.14662638209091158No Hit
GTCTAAGCAGGTCTGGTTGCATTTGTGTTTGGTTTCGAAGCATCCGTTTC26680.14361203649726584No Hit
CCATATAAACAATAAAAATAGCTAGCATTAATGTTACAGCCAAACTAGAA26120.14059769090362007No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT25700.13833693170838574No Hit
GTCTTATCATCTCTTACCATCTTGTAAAAGGTGCCTCCTGTTTTGTTGTG25690.1382831041084992No Hit
ACCATAGAGTACTCTTCAACATTGAACCCTAGTTGAGGTATTTTGGCGTA25560.13758334530997432No Hit
GAATAAACACCCACATTTCAAACGTATTGAGACAATCACAGTTATAGTGT25310.136237655312811No Hit
GTTATAGTGTCTATTTTGTTGCTTTAATAATCGAGGTCATCATAATCCTC25180.13553789651428613No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA25050.1348381377157612No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA24390.13128551612325012No Hit
TCTGGGGACTGTGCTTCCCGCAAAAGTGCTGATTAATGAAGGATCAAGTC23780.12800203253017173No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT23310.12547213533550475No Hit
GTTGTAGACGCTCTTCTTTATCTCTGTCGGGGTCTGTTTCTTTTGGGTTT23290.12536448013573168No Hit
TTTTAATGCTGATCTAGGCTTGAATTCTGTGCCTGTTAATGCAGACAAAA22150.11922813374866709No Hit
GTCTACAGCAGAAGCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAA21900.11788244375150381No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG21750.11707502975320584No Hit
GCATTAAAGGTGCCAGCAGCTATCCTGTCTAAGCAGGTCTGGTTGCATTT21390.11513723615729071No Hit
TGATAAGACCATCTACTTCAGCCCTATAAGAATTACCTTTTTGAAAGAAG21250.11438364975887927No Hit
GAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAAGA20130.10835495857158775No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT20070.10803199297226855No Hit
CTATAAGAATTACCTTTTTGAAAGAAGAGGTGAAAACAATGTACAAAACC20060.10797816537238203No Hit
GCATTAATGTTACAGCCAAACTAGAAGCAGCAGTTGAGTAATAGAGCAGT20060.10797816537238203No Hit
GTCCCCAGAAGATCAGGTGCGACTGGTGTTGCAATCAAAGGAGGCGGAAC20050.1079243377724955No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC19840.10679395817487834No Hit
CCTTCTACCTGTTCCTTTGCTGGAACATGGAAACCCTTGCATTTTAATGC19570.10534061297794199No Hit
CTATCTGCCATTGCTCTTCCTATAAATCGAATGGCTTCAGCCACTAAAGT19260.10367195738145953No Hit
GTAAAGAGCCATGGCTTCATACCCAACCATAGAGTACTCTTCAACATTGA19230.10351047458179992No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC19070.10264923298361542No Hit
GGGTTAGGTCTTCAATGTCTGCAATCCCTGGATTTCTGCTTCCGATCACT18960.10205712938486358No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGAGT100.0070859655145.036836
AGCACGC5600.0124.354121
GCACGCA5650.0123.227082
CACGCAC5700.0122.146153
CGGTGCA800.090.655342
CGCACTT8400.084.609375
ACGCACT8450.084.110994
AAACACG3200.081.587615
ACAACGC2700.080.554338
GGTGCAT950.076.341343
CAACGCA2850.076.314639
CGTGCAA406.6536464E-572.524283
ACGTGCA1900.072.524282
ACACGTG1450.069.998937
GCGGTGC1900.064.904111
CAAACCG702.3306711E-762.161995
AACCGTC702.3358734E-762.1419147
CACGTGC2700.061.7932431
AACACGA2600.061.3617366
CCGTCCA753.7633617E-757.9991159
Produced by FastQC (version 0.11.5)