FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_9932_2_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9932_2_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1869067
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG87400.4676129855163031No Hit
GTATAAATACTGGGACAATTGACAAAACACCGGAAGAAATAACTTCTGGA69870.37382287526343355No Hit
TCATAATCCTCTGCTGTGTCCCTCCCAAAGAAGAAATTGGGGATGGTCTG53220.2847409964436802No Hit
GTATTTATACCGTCAATATCCATGTTGGACATTTTGATTTTCAGTTCTTT51490.2754850414672133No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT43360.23198740334081122No Hit
CAGTTATAGTGTCTATTTTGTTGCTTTAATAATCGAGGTCATCATAATCC42830.2291517639549572No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT37290.19951130697829453No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT35250.18859677047425266No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA32700.17495359984420034No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC31250.16719571850554316No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT30210.16163144499367865No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG30120.16114992132438272No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG28780.153980568914865No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT27810.14879081381245293No Hit
TTCTTATAGGGCTGAAGTAGATGGTCTTATCATCTCTTACCATCTTGTAA27460.14691822176519087No Hit
GTCTAAGCAGGTCTGGTTGCATTTGTGTTTGGTTTCGAAGCATCCGTTTC26840.1436010587100409No Hit
CCATATAAACAATAAAAATAGCTAGCATTAATGTTACAGCCAAACTAGAA26240.14039090091473447No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT25830.13819729308794174No Hit
GTCTTATCATCTCTTACCATCTTGTAAAAGGTGCCTCCTGTTTTGTTGTG25790.13798328256825465No Hit
ACCATAGAGTACTCTTCAACATTGAACCCTAGTTGAGGTATTTTGGCGTA25750.13776927204856754No Hit
GAATAAACACCCACATTTCAAACGTATTGAGACAATCACAGTTATAGTGT25450.13616419315091433No Hit
GTTATAGTGTCTATTTTGTTGCTTTAATAATCGAGGTCATCATAATCCTC25290.13530815107216596No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA25140.13450561162333935No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA24490.13102794067842405No Hit
TCTGGGGACTGTGCTTCCCGCAAAAGTGCTGATTAATGAAGGATCAAGTC23950.12813879866264827No Hit
GTTGTAGACGCTCTTCTTTATCTCTGTCGGGGTCTGTTTCTTTTGGGTTT23410.1252496566468725No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT23390.12514265138702893No Hit
TTTTAATGCTGATCTAGGCTTGAATTCTGTGCCTGTTAATGCAGACAAAA22310.11936436735547737No Hit
GTCTACAGCAGAAGCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAA22010.11775928845782414No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG21880.1170637542688411No Hit
GCATTAAAGGTGCCAGCAGCTATCCTGTCTAAGCAGGTCTGGTTGCATTT21510.11508415696173545No Hit
TGATAAGACCATCTACTTCAGCCCTATAAGAATTACCTTTTTGAAAGAAG21330.11412110962314352No Hit
GAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAAGA20250.10834282559159195No Hit
CTATAAGAATTACCTTTTTGAAAGAAGAGGTGAAAACAATGTACAAAACC20200.10807531244198308No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT20200.10807531244198308No Hit
GTCCCCAGAAGATCAGGTGCGACTGGTGTTGCAATCAAAGGAGGCGGAAC20170.10791480455221776No Hit
GCATTAATGTTACAGCCAAACTAGAAGCAGCAGTTGAGTAATAGAGCAGT20140.10775429666245245No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC19910.10652373617425165No Hit
CCTTCTACCTGTTCCTTTGCTGGAACATGGAAACCCTTGCATTTTAATGC19720.10550718620573793No Hit
CTATCTGCCATTGCTCTTCCTATAAATCGAATGGCTTCAGCCACTAAAGT19340.10347408626871055No Hit
GTAAAGAGCCATGGCTTCATACCCAACCATAGAGTACTCTTCAACATTGA19330.10342058363878875No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC19150.10245753630019683No Hit
GGGTTAGGTCTTCAATGTCTGCAATCCCTGGATTTCTGCTTCCGATCACT19010.10170849948129201No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGCG100.0070837415145.052083
GTGCACG100.0070916796144.99776145
GCACGCA5750.0122.348282
AGCACGC5700.0122.168731
CACGCAC5850.0121.496613
AAGCGGT458.367351E-11112.818282
GAAGCGG1000.0108.8065261
AGCGGTG554.1472958E-1092.305873
CGGTGCA554.1472958E-1092.305872
GCGGTGC1450.090.046781
AAGCGGA608.276402E-1084.6137162
ACGCACT8900.083.934634
CGGTGCC703.092282E-1182.88692
CGCACTT8850.082.76545
TATCTTC554.3855835E-879.1193163
TATGCTG406.6652115E-572.49888145
AAACACG3950.071.604165
ACAACGC2850.068.683158
GGTGCAT754.843969E-967.690973
CAACGCA2900.067.4989559
Produced by FastQC (version 0.11.5)