FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_9932_1_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9932_1_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2076940
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT135490.6523539437826803No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC115930.5581769333731355No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT111150.5351623060849133No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG107420.5172031931591669No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA106420.5123884175758567No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT93950.4523481660519803No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA92800.4468111741311737No Hit
GTGTTAAGTCATCCATGCTTTGACATTTTTCAGGAAACCTTCTATCTTAG87300.42032990842296836No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT82850.39890415707723864No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA81780.39375234720309693No Hit
GTAGTAACAAGAGCATCATCACTTCTGTTGGATGTTTGTGAGATGTCATC71240.3430046125550088No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC67300.32403439675676715No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA66900.32210848652344315No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT66140.3184492570801275No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT65880.3171974154284669No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG64600.31103450268182997No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT62260.29976792781688444No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA61650.2968309147110653No Hit
ATCAAAGGCTGTGCCCTATCCTGGCTTAGGTCTTACCTCTCTGACCGACT59210.2850828622877888No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT57070.27477924253950525No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA56030.2697718759328628No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT55650.2679422612112049No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG55240.2659682032220478No Hit
ACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA53870.25937196067291307No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG52920.2547979238687685No Hit
AGTAGAAACAACGCACTTTGGTTGTTGGTATCAAAGGCTGTGCCCTATCC50060.24102766570050171No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT49340.23756102728051845No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT49100.23640548114052404No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC47770.23000182961472165No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG46060.22176856336726145No Hit
ATCCTGGCTTAGGTCTTACCTCTCTGACCGACTTCAATTTGTACATGTCC45870.22085375600643253No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA44690.21517232081812668No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT44310.21334270609646883No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT44050.21209086444480824No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT43380.2088649648039905No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG40400.1945169335657265No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG39970.19244658006490317No Hit
GCGCTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTC38440.1850799734224388No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA38170.18377998401494505No Hit
ATTTAAATGTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAA38080.18334665421244717No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC37670.18137259622329002No Hit
GAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAGAATCTGGAATG36110.17386154631332634No Hit
AAATTATTCAATGCAAGTAAAACTAGGAACACTCTGTGCTTTGTGCGAAA35910.17289859119666431No Hit
TTCATGAAGCATTTGAAATAGCAGAAGGCCATGAAAGCTCAGCGCTACTA34510.16615790538003022No Hit
TTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGATCTCAATACT34160.16447273392587172No Hit
CTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCA33550.16153572082005258No Hit
CCTTAAGGCTCAGTGCCTGGGCCCCTGCTCTTCTCTTTGTACATGCTACC33330.16047647019172437No Hit
CCTTTATTCTTATTTTCATGTTTACTCTTCTTTTTATTTGATTTAAATGT32680.15734686606257284No Hit
ATCTTAGGTAGTTTAGGTTGGTATAATTTCATCAGGTTTCATGGATAGGT32200.15503577378258399No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCCTT32180.1549394782709178No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC32050.15431355744508748No Hit
GTGCCAGGAGTGAGACGGGAAATGCAGATGGTCTCAGCTATGAACACAGC31630.15229135170009725No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT31580.15205061292093175No Hit
ATCTGGAATGGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTT30760.1481024969426175No Hit
GCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAG30490.14680250753512378No Hit
TCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGT30460.14665806426762448No Hit
ATTTTGATCTGTGTTAAGTCATCCATGCTTTGACATTTTTCAGGAAACCT30030.14458771076680116No Hit
ACTAGGAACACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACA29890.14391364218513775No Hit
TTGTTAGAGAGTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTG29800.14348031238263984No Hit
CTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATAG29060.1399173784509904No Hit
TAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAATGCAGA28900.1391470143576608No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT28590.13765443392683466No Hit
CATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGG27600.1328878060993577No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT27260.1312507824010323No Hit
CCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGT27250.13120263464519918No Hit
CTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTAGCACTTCCATTA27140.1306730093310351No Hit
AAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAAC26390.12706192764355254No Hit
GGGGAAAGGAGAAGACGTCCAAAAACTGGCAGAAGAACTGCAAAGCAACA26330.12677304110855392No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA26330.12677304110855392No Hit
GCCCTATCCTGGCTTAGGTCTTACCTCTCTGACCGACTTCAATTTGTACA26240.12633971130605604No Hit
CCTTTCCCCATTCCATTCATTGTTTTTGCTGTGTTCATAGCTGAGACCAT26070.1255211994568933No Hit
CTAGGAACACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAG25770.1240767667819003No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCC25440.12248789083940798No Hit
CCATAGAGCTCTGCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCC24920.11998420753608675No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG24800.11940643446608955No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA24680.11882866139609233No Hit
GTAGAAACAACAGCAACAAAAAAGAAGGGCCTGATTCTGGCTGAGAGAAA24630.11858792261692684No Hit
GATTACAATTTGCTTAAATGCATAGTAAGAAATACAGTAAAAATTGAATT24200.11651756911610349No Hit
TGTCCAAAGTGAGTCATCCAGTCATACAACAGTAAGTCAAGGAGTTCCTT24190.11646942136027041No Hit
GTTTAGTACTTACAAACTATTTTGATCTGTGTTAAGTCATCCATGCTTTG23830.11473610215027878No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCAT23780.11449536337111328No Hit
CTTTGGTTGTTGGTATCAAAGGCTGTGCCCTATCCTGGCTTAGGTCTTAC23710.11415832908028156No Hit
GCCTGATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAA23320.11228056660279064No Hit
GTTTTTGCTGTGTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCC23080.11112502046279622No Hit
CAAGAGCATCATCACTTCTGTTGGATGTTTGTGAGATGTCATCGAGTGTG22780.10968058778780322No Hit
AACACGAGCATCATCACTTCTGTTGGATGTTTGTGAGATGTCATCGAGTG22680.10919911022947221No Hit
GTCATACAACAGTAAGTCAAGGAGTTCCTTAAGGCTCAGTGCCTGGGCCC22510.1083805983803095No Hit
GACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAA22500.10833245062447638No Hit
AAAAAGAAGGGCCTGATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAG22110.10645468814698547No Hit
GCATTTGAAATAGCAGAAGGCCATGAAAGCTCAGCGCTACTATATTGTCT21790.10491395996032626No Hit
GGCCATGAAAGCTCAGCGCTACTATATTGTCTCATGGTCATGTACCTGAA21690.10443248240199525No Hit
ACATTGGAGTATTGAGATCCCTTGGGGCAAGTCAGAAGAATGGGGAAGGA21410.10308434523866844No Hit
ATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAGAATCTGGAA21330.10269916319200362No Hit
CACGTGCATCATCACTTCTGTTGGATGTTTGTGAGATGTCATCGAGTGTG21300.10255471992450431No Hit
GGAGTATTGAGATCCCTTGGGGCAAGTCAGAAGAATGGGGAAGGAATTGC21170.10192879909867401No Hit
GCTTAAATGCATAGTAAGAAATACAGTAAAAATTGAATTTAATGCGATTC21150.1018325035870078No Hit
CTTCTGTTGGATGTTTGTGAGATGTCATCGAGTGTGAGTAAATGGCTGGA21140.10178435583117472No Hit
AATCTGGAATGGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATT21030.10125473051701062No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT20910.10067695744701341No Hit
ACCCTTAGGTGCTGTAATGAGGAAGCATGGGTTTCCATTGCTATGCTGAT20890.10058066193534719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCA750.0116.0212862
AGCACGC11500.0113.526421
GCACGCA11700.0111.558932
CACGCAC11900.0109.074643
CATAACG203.8601283E-4108.796161
GGTGCAT800.0108.769963
GCGGTGC1850.094.093971
GGAGCGT259.38134E-487.015963
TAACGCA259.38134E-487.015963
ATAACGC259.38134E-487.015962
GAGCACG406.6508794E-572.530771
CGCATAC300.001934997972.509816
GAAACAC9300.071.733584
TGCCTTA1650.070.315932
ACAACGC24250.069.958128
AACAACG24400.069.825187
TAGTAAC25900.069.713562
AAACACG9800.068.0720755
CAACGCA24950.067.995379
CGGTGCC1009.094947E-1265.261972
Produced by FastQC (version 0.11.5)