FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_9861_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9861_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1644077
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT86050.5233939772893849No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT38010.23119355115362603No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT36910.22450286695817775No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA35510.2159874507094254No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT33700.2049782339878242No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC31300.1903803775613916No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT30580.1860010206334618No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG30130.1832639225535057No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG29260.1779721995989239No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA29100.17699900917049508No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCTCCATTCTTCTGA26030.1583259178250167No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT25780.15680530778059665No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA25030.15224347764733645No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT24990.15200018004022925No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG24010.1460393886661026No Hit
GTATAAATACTGGGACAATTGACAAAACACCGGAAGAAATAACTTCTGGA21540.13101576142723242No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT21120.12846113655260671No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT20170.12268281838381048No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC19740.12006736910740799No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC19090.11611378299191584No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT19000.11556636337592462No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA18800.11434987534038857No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAGAGGAGAAGACGT18360.11167360166220924No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC18330.11149112845687884No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA18320.11143030405510206No Hit
ATTACATCCTTTGCAATTCCTTCTCCATTCTTCTGACTTGCCCCAAGGGA17750.10796331315382429No Hit
GTCATAATGGGAGCCAATGACGTAAGTGAATTAGAATCACAAGCACAACT17570.10686847392184186No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT17420.10595610789518983No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG17070.10382725383300173No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT16650.10127262895837605No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT16500.100360262931724No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCCC100.0070863306145.033833
AGCACGC10650.0125.979851
GCACGCA10650.0125.2874452
CACGCAC10750.0124.121973
CGGTGCA850.0110.9082262
CTATATA203.861516E-4108.78531
GGTGCAT950.099.233673
ACGCACT13600.099.177544
CGCACTT13600.099.174535
GAAACAC3200.092.91234
AAACACG3200.092.909475
CTCTAAA259.3757355E-487.028241
CGTGCTC259.3882065E-486.99912145
ACAACGC2900.084.9991468
CAACGCA2950.083.558489
AACACGT1150.075.665226
GAGCAGA1250.075.424481
AAGCACG1450.075.0243451
GCGGTGC2150.074.210131
TCTAAAT300.001934173972.5169142
Produced by FastQC (version 0.11.5)