FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_9841_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9841_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences650782
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATAGTAGATCTATAGAAGTATAAATCTATAAATGAAACCCAATCA84541.2990525245012923No Hit
GATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCCATCAGACCCA80431.235897735339945No Hit
AGTAGAAACAACGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCC68011.0450504162684278No Hit
GTATAAATCTATAAATGAAACCCAATCAGCATCGTTCTCAATACTGTTAC57160.8783279193339705No Hit
CTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCCATCAGACC56650.8704911936716137No Hit
ATCTATAGTAGATCTATAGAAGTATAAATCTATAAATGAAACCCAATCAG56570.869261903371636No Hit
CTATAGAAGTATAAATCTATAAATGAAACCCAATCAGCATCGTTCTCAAT55590.8542030971969108No Hit
GGATAATGTAACTGCCGTTTTCCATCAGACCCAGTTCACTCACCGTCCCA42860.6585922782129807No Hit
ACAACGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCC41510.6378480044008592No Hit
CAACAGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCC41340.635235762513407No Hit
ATAGTAGATCTATAGAAGTATAAATCTATAAATGAAACCCAATCAGCATC34370.5281338451278615No Hit
GTATTTGGCAAAGGCACATGTCTTGTGTTTTTAATCAGTCCAGAGTGGAG32630.5013967811033495No Hit
CTGTAGAACTGCTTTCACAGTCCAGAATGGAAAGGAGACAATCACTCCAA30730.47220113647888234No Hit
TCTCAATACTGTTACTCTCTGGTCTGCATCAGGATCACAAGACAGAACTC30320.4659010236914973No Hit
GTTTGGGATAATGTAACTGCCGTTTTCCATCAGACCCAGTTCACTCACCG30250.46482539467901696No Hit
GTCCAGAATGGAAAGGAGACAATCACTCCAATTCCAAACCCAAATGTGCA29950.460215556054101No Hit
GATCTATAGAAGTATAAATCTATAAATGAAACCCAATCAGCATCGTTCTC27860.4281003469671872No Hit
CTATAAATGAAACCCAATCAGCATCGTTCTCAATACTGTTACTCTCTGGT27850.42794668567968996No Hit
GAATAAAACGTATTCATCAAGAGCTGTTTTACCTGTTTACATTACTAAAT27040.4155001213924171No Hit
GTCCTATTTATGAGAAGAAAAACCAATCAGGGTATCAGAGAGCATTCTTC26240.40320721839264145No Hit
TGGTAGTACCATACGAGGACAGCATCAGTGTGGGGTTCTTCTTTCAGACT25490.39168262183035174No Hit
GACAGGGACTGTCCACCACTGACATCCGGAGGATCAACAAACTCTATGGA25250.3879947509304191No Hit
ATATTGAGGTCATCTCAGTGATTTATTTTCCAGTATTGTGAATATTTTAG23940.36786512226828644No Hit
ATCTATAGAAGTATAAATCTATAAATGAAACCCAATCAGCATCGTTCTCA23420.3598747353184323No Hit
AAACAACGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTT22760.3497330903436174No Hit
GTGTGGGGTTCTTCTTTCAGACTCGATCTGTCCTATTTATGAGAAGAAAA21230.32622291335654646No Hit
GTCCACCACTGACATCCGGAGGATCAACAAACTCTATGGATGCTGTGAGT20920.32145941344413337No Hit
CTCCATATGACTACACTTCTGTGATGCATTATGGGAGGTAAGAAAATGGG20380.3131617039192848No Hit
GGGTATCAGAGAGCATTCTTCCATCGTGATTCCTGTCATTTCAGTGTCCC20010.3074762362818886No Hit
GTAGAAACACGTGCTAATCTGACAGATCTATAGTAGATCTATAGAAGTAT19950.3065542685569054No Hit
CTCTATGGATGCTGTGAGTATGGTGTTCAAACTCCATCAGATACATGGAA19600.3011761234945035No Hit
GTTCACTCACCGTCCCACAGTATTTGGCAAAGGCACATGTCTTGTGTTTT19490.29948584933203437No Hit
TACGAGGACAGCATCAGTGTGGGGTTCTTCTTTCAGACTCGATCTGTCCT19280.29625896229459325No Hit
CCATATGACTACACTTCTGTGATGCATTATGGGAGGTAAGAAAATGGGAC18760.2882685753447391No Hit
GGGATATTGAGGTCATCTCAGTGATTTATTTTCCAGTATTGTGAATATTT17960.27597567234496345No Hit
GTAGAAACACGAGCTAATCTGACAGATCTATAGTAGATCTATAGAAGTAT17480.26859993054509806No Hit
ATATAAATCATCATTTAGCAAGTTGTCATGTGGTAGTACCATACGAGGAC17120.263068124195199No Hit
GCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCCATCAG16570.2546167533828532No Hit
ATGTAACTGCCGTTTTCCATCAGACCCAGTTCACTCACCGTCCCACAGTA16570.2546167533828532No Hit
CAACGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCCA16120.24770199544547944No Hit
CTCCAATTCCAAACCCAAATGTGCAAATTGGACAGAGACAGGGACTGTCC15450.23740668918316735No Hit
CTATAGTAGATCTATAGAAGTATAAATCTATAAATGAAACCCAATCAGCA15080.2317212215457711No Hit
GATTACAAGAGCATCATAAACATCCAAACACTCTATGTGTACAGACAAGA14780.2271113829208552No Hit
GTTTTACCTGTTTACATTACTAAATAAAAATATGTTTTATAAAACTTTTA14430.22173323785845336No Hit
TTCCAGTATTGTGAATATTTTAGATACGATGCTCACAAGACAAATAAATA14120.21696973794604033No Hit
GCATCAGTGTGGGGTTCTTCTTTCAGACTCGATCTGTCCTATTTATGAGA13930.2140501734835936No Hit
CATGGAAAGAGGGCATCATTTTGTTTGTCTTTGGGGACACTGAAATGACA13650.2097476574336721No Hit
GTAGAAACAACGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCG13560.20836470584619737No Hit
CTTTCCATGTATCTGATGGAGTTTGAACACCATACTCACAGCATCCATAG13500.20744273812121414No Hit
GCTTTCACAGTCCAGAATGGAAAGGAGACAATCACTCCAATTCCAAACCC13490.207289076833717No Hit
GTTCAAATTTCCTTCTGTAGAACTGCTTTCACAGTCCAGAATGGAAAGGA13450.20667443168372818No Hit
AATCAGCATCGTTCTCAATACTGTTACTCTCTGGTCTGCATCAGGATCAC13450.20667443168372818No Hit
GTTCTCAATACTGTTACTCTCTGGTCTGCATCAGGATCACAAGACAGAAC13330.20483049623376184No Hit
CTGTTACTCTCTGGTCTGCATCAGGATCACAAGACAGAACTCCACTCTGG13170.20237191563380671No Hit
TCCATAGAGTTTGTTGATCCTCCGGATGTCAGTGGTGGACAGTCCCTGTC12810.19684010928390766No Hit
AATCTGACAGATCTATAGTAGATCTATAGAAGTATAAATCTATAAATGAA12550.19284491580898058No Hit
GTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTGGTAACTGTGT12180.18715944817158436No Hit
CTTCAGCACAGGTGGGGCCGTTGTAGGGCAGATCACACAGGCAGGTGAAG11880.18254960954666846No Hit
GTGCTAATCTGACAGATCTATAGTAGATCTATAGAAGTATAAATCTATAA11750.18055201280920494No Hit
TTGTAGGGCAGATCACACAGGCAGGTGAAGCTGGCCACACCATCCACACA11720.18009102894671333No Hit
GTATCAGAGAGCATTCTTCCATCGTGATTCCTGTCATTTCAGTGTCCCCA11620.17855441607174138No Hit
GTCATGTGGTAGTACCATACGAGGACAGCATCAGTGTGGGGTTCTTCTTT11600.178247093496747No Hit
GTCCCTGTCTCTGTCCAATTTGCACATTTGGGTTTGGAATTGGAGTGATT11530.17717146448426663No Hit
GTGGTAGTACCATACGAGGACAGCATCAGTGTGGGGTTCTTCTTTCAGAC11370.1747128838843115No Hit
GTCCCACAGTATTTGGCAAAGGCACATGTCTTGTGTTTTTAATCAGTCCA11350.17440556130931711No Hit
ACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCCATCAGACCCAGTTC11340.1742519000218199No Hit
GGGATAATGTAACTGCCGTTTTCCATCAGACCCAGTTCACTCACCGTCCC11250.17286894843434514No Hit
GTTTACATTACTAAATAAAAATATGTTTTATAAAACTTTTACAAATCACT11130.1710250129843788No Hit
CAATACTGTTACTCTCTGGTCTGCATCAGGATCACAAGACAGAACTCCAC10970.16856643238442368No Hit
TAGTAGATCTATAGAAGTATAAATCTATAAATGAAACCCAATCAGCATCG10920.1677981259469377No Hit
GCATTATGGGAGGTAAGAAAATGGGACCCACTGAATAAAACGTATTCATC10860.1668761582219545No Hit
TTCCTAGAGTGGCCACTAGGAGGAGTTCCAGCCTCAGTCAGTAATGATCT10810.16610785178446855No Hit
CAACAGCAAAGAGGGATATTGAGGTCATCTCAGTGATTTATTTTCCAGTA10690.16426391633450219No Hit
GTCCAGAGTGGAGTTCTGTCTTGTGATCCTGATGCAGACCAGAGAGTAAC10680.164110255047005No Hit
CTCTAGGAATAGCCAGTTGGAGAGGTGGTGTTCTCAGGGTAGAGTAGCCT10660.16380293247201058No Hit
AATAAATATAAATCATCATTTAGCAAGTTGTCATGTGGTAGTACCATACG10640.1634956098970162No Hit
GTGAGTATGGTGTTCAAACTCCATCAGATACATGGAAAGAGGGCATCATT10550.16211265830954144No Hit
GTTACTCTCTGGTCTGCATCAGGATCACAAGACAGAACTCCACTCTGGAC10470.16088336800956388No Hit
TTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCCATCAGACCCAGT10470.16088336800956388No Hit
TTATAAAACTTTTACAAATCACTGTTCAAATTTCCTTCTGTAGAACTGCT10070.15473691650967605No Hit
GTACCATACGAGGACAGCATCAGTGTGGGGTTCTTCTTTCAGACTCGATC9920.15243199719721812No Hit
GAGGGATATTGAGGTCATCTCAGTGATTTATTTTCCAGTATTGTGAATAT9830.15104904560974333No Hit
GAGACAGGGACTGTCCACCACTGACATCCGGAGGATCAACAAACTCTATG9780.15028073917225737No Hit
GGATTGAAAGGAAGAAAGAGTGACGGAGCAAAAAAGCGAAAGGGAGAGTG9600.14751483599730786No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG9460.1453635779723471No Hit
AGTAGAAACAACGCACCAACAACCTGAACACTCCATATGACTACACTTCT9440.14505625539735273No Hit
GCAAAGAGGGATATTGAGGTCATCTCAGTGATTTATTTTCCAGTATTGTG9230.1418293683599116No Hit
GTAGTAACAAGAGCAACAACAGCAACAACATAACAATGTAAGAGACCTTG9200.14136838449742No Hit
GAGTATGGTGTTCAAACTCCATCAGATACATGGAAAGAGGGCATCATTTT8890.13660488458500697No Hit
GTAGAAACAACAGCAAAGAGGGATATTGAGGTCATCTCAGTGATTTATTT8840.13583657814752098No Hit
CACGTGCTAATCTGACAGATCTATAGTAGATCTATAGAAGTATAAATCTA8790.135068271710035No Hit
CCATACTCACAGCATCCATAGAGTTTGTTGATCCTCCGGATGTCAGTGGT8590.1319950459600911No Hit
ATTGTGAATATTTTAGATACGATGCTCACAAGACAAATAAATATAAATCA8550.1313804008101023No Hit
CTATGGATGCTGTGAGTATGGTGTTCAAACTCCATCAGATACATGGAAAG8480.13030477179762193No Hit
GTTGTCATGTGGTAGTACCATACGAGGACAGCATCAGTGTGGGGTTCTTC8390.12892182021014717No Hit
CTCTATGTGTACAGACAAGATCAACTCTAAATGACTGAGCATTCAGATGC8310.1276925299101696No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA8310.1276925299101696No Hit
ATTTATGAGAAGAAAAACCAATCAGGGTATCAGAGAGCATTCTTCCATCG8180.12569493317270605No Hit
CTTCTGTGATGCATTATGGGAGGTAAGAAAATGGGACCCACTGAATAAAA7970.12246804613526496No Hit
CTAAATGACTGAGCATTCAGATGCTTGCTTTTCTGTGAACGGCCTCAGCT7910.12154607841028178No Hit
TAATGTAACTGCCGTTTTCCATCAGACCCAGTTCACTCACCGTCCCACAG7840.12047044939780141No Hit
GTAGTAACAAGAGCATGTGAGATTATTTTTGAATTGACAATTATTTTAAA7820.12016312682280703No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCAT7790.11970214296031544No Hit
GTATTGTGAATATTTTAGATACGATGCTCACAAGACAAATAAATATAAAT7660.1177045462228519No Hit
GTGGTGGACAGTCCCTGTCTCTGTCCAATTTGCACATTTGGGTTTGGAAT7640.11739722364785748No Hit
ATACTGTTACTCTCTGGTCTGCATCAGGATCACAAGACAGAACTCCACTC7620.1170899010728631No Hit
GTTCTACCCTCAGGCACATGGTCATGTTGAGATCAAGGCTACTCTACCCT7420.11401667532291919No Hit
GCATCGTTCTCAATACTGTTACTCTCTGGTCTGCATCAGGATCACAAGAC7410.11386301403542201No Hit
TCTCAGTGATTTATTTTCCAGTATTGTGAATATTTTAGATACGATGCTCA7400.1137093527479248No Hit
ACAACAGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTC7390.1135556914604276No Hit
TCACAAGACAAATAAATATAAATCATCATTTAGCAAGTTGTCATGTGGTA7300.11217273987295284No Hit
GTTCAAACTCCATCAGATACATGGAAAGAGGGCATCATTTTGTTTGTCTT7270.11171175601046125No Hit
AACAACGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTC7250.11140443343546688No Hit
AACCCAATCAGCATCGTTCTCAATACTGTTACTCTCTGGTCTGCATCAGG7240.11125077214796968No Hit
ATCAGACCCAGTTCACTCACCGTCCCACAGTATTTGGCAAAGGCACATGT7200.11063612699798088No Hit
AATGAAACCCAATCAGCATCGTTCTCAATACTGTTACTCTCTGGTCTGCA7110.10925317541050612No Hit
GGAGACAATCACTCCAATTCCAAACCCAAATGTGCAAATTGGACAGAGAC7020.10787022382303137No Hit
GAGCTAATCTGACAGATCTATAGTAGATCTATAGAAGTATAAATCTATAA7020.10787022382303137No Hit
GGCACATGTCTTGTGTTTTTAATCAGTCCAGAGTGGAGTTCTGTCTTGTG6970.1071019173855454No Hit
GTAGAAACAACAGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCC6890.10587262708556783No Hit
GTAGAACTGCTTTCACAGTCCAGAATGGAAAGGAGACAATCACTCCAATT6880.10571896579807062No Hit
GTAGTACCATACGAGGACAGCATCAGTGTGGGGTTCTTCTTTCAGACTCG6790.10433601421059586No Hit
TGACTACACTTCTGTGATGCATTATGGGAGGTAAGAAAATGGGACCCACT6760.10387503034810427No Hit
CCATCAGACCCAGTTCACTCACCGTCCCACAGTATTTGGCAAAGGCACAT6750.10372136906060707No Hit
ATTTTAGATACGATGCTCACAAGACAAATAAATATAAATCATCATTTAGC6590.10126278846065197No Hit
ACTCTGGACTGATTAAAAACACAAGACATGTGCCTTTGCCAAATACTGTG6560.10080180459816036No Hit
GACCCAGTTCACTCACCGTCCCACAGTATTTGGCAAAGGCACATGTCTTG6520.1001871594481716No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATAGG151.2298743E-4144.99808145
TAACACG553.6379788E-12105.4774555
TTGCACC259.3714404E-487.032294
TGAAAGG1850.074.475645
CCTTAGA608.006646E-872.52692
GAGGGCT608.027928E-872.499047
AACAACG23800.067.015927
GGTTGTC554.060332E-665.908218
GCACGCA2350.064.811272
GTCATAA350.003560706362.1659161
CGTGCAA702.3272696E-762.1659163
GAAACAA28450.060.9183355
GTTGTCC606.803466E-660.4158679
CCCAAGC851.3005774E-859.7280351
AAGCAGC851.3005774E-859.7280354
GCCTTAG753.7495192E-758.0215231
TTAAGTG1154.0017767E-1156.7514655
TGTACTT651.0933038E-555.768494145
ACAACGC29100.055.0594068
TATAGGG400.006041066354.395182
Produced by FastQC (version 0.11.5)