FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_9841_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9841_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655235
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATAGTAGATCTATAGAAGTATAAATCTATAAATGAAACCCAATCA84991.2970918830648546No Hit
GATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCCATCAGACCCA80911.2348241470617412No Hit
AGTAGAAACAACGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCC68421.0442055140522104No Hit
GTATAAATCTATAAATGAAACCCAATCAGCATCGTTCTCAATACTGTTAC57520.8778529840438927No Hit
ATCTATAGTAGATCTATAGAAGTATAAATCTATAAATGAAACCCAATCAG56980.8696116660434806No Hit
CTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCCATCAGACC56920.8686959640434347No Hit
CTATAGAAGTATAAATCTATAAATGAAACCCAATCAGCATCGTTCTCAAT55900.8531290300426564No Hit
GGATAATGTAACTGCCGTTTTCCATCAGACCCAGTTCACTCACCGTCCCA43090.6576266530328814No Hit
ACAACGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCC41790.6377864430318892No Hit
CAACAGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCC41570.6344288690317215No Hit
ATAGTAGATCTATAGAAGTATAAATCTATAAATGAAACCCAATCAGCATC34620.528360054026418No Hit
GTATTTGGCAAAGGCACATGTCTTGTGTTTTTAATCAGTCCAGAGTGGAG32830.5010416110250521No Hit
CTGTAGAACTGCTTTCACAGTCCAGAATGGAAAGGAGACAATCACTCCAA30920.4718917640235946No Hit
TCTCAATACTGTTACTCTCTGGTCTGCATCAGGATCACAAGACAGAACTC30470.4650239990232512No Hit
GTTTGGGATAATGTAACTGCCGTTTTCCATCAGACCCAGTTCACTCACCG30390.4638030630231902No Hit
GTCCAGAATGGAAAGGAGACAATCACTCCAATTCCAAACCCAAATGTGCA30150.460140255023007No Hit
CTATAAATGAAACCCAATCAGCATCGTTCTCAATACTGTTACTCTCTGGT27980.42702236602135113No Hit
GATCTATAGAAGTATAAATCTATAAATGAAACCCAATCAGCATCGTTCTC27980.42702236602135113No Hit
GAATAAAACGTATTCATCAAGAGCTGTTTTACCTGTTTACATTACTAAAT27220.4154234740207712No Hit
GTCCTATTTATGAGAAGAAAAACCAATCAGGGTATCAGAGAGCATTCTTC26380.40260364602013016No Hit
TGGTAGTACCATACGAGGACAGCATCAGTGTGGGGTTCTTCTTTCAGACT25640.39130998801956546No Hit
GACAGGGACTGTCCACCACTGACATCCGGAGGATCAACAAACTCTATGGA25420.3879524140193976No Hit
ATATTGAGGTCATCTCAGTGATTTATTTTCCAGTATTGTGAATATTTTAG24030.3667386510183369No Hit
ATCTATAGAAGTATAAATCTATAAATGAAACCCAATCAGCATCGTTCTCA23520.35895518401794774No Hit
AAACAACGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTT22910.34964554701748224No Hit
GTGTGGGGTTCTTCTTTCAGACTCGATCTGTCCTATTTATGAGAAGAAAA21360.3259899120162995No Hit
GTCCACCACTGACATCCGGAGGATCAACAAACTCTATGGATGCTGTGAGT21000.3204957000160248No Hit
CTCCATATGACTACACTTCTGTGATGCATTATGGGAGGTAAGAAAATGGG20490.3127122330156356No Hit
GGGTATCAGAGAGCATTCTTCCATCGTGATTCCTGTCATTTCAGTGTCCC20150.3075232550153762No Hit
GTAGAAACACGTGCTAATCTGACAGATCTATAGTAGATCTATAGAAGTAT20040.30584446801529225No Hit
CTCTATGGATGCTGTGAGTATGGTGTTCAAACTCCATCAGATACATGGAA19680.3003502560150175No Hit
GTTCACTCACCGTCCCACAGTATTTGGCAAAGGCACATGTCTTGTGTTTT19640.29973978801498696No Hit
TACGAGGACAGCATCAGTGTGGGGTTCTTCTTTCAGACTCGATCTGTCCT19350.2953138950147657No Hit
CCATATGACTACACTTCTGTGATGCATTATGGGAGGTAAGAAAATGGGAC18870.28798827901439944No Hit
GGGATATTGAGGTCATCTCAGTGATTTATTTTCCAGTATTGTGAATATTT18050.2754736850137737No Hit
GTAGAAACACGAGCTAATCTGACAGATCTATAGTAGATCTATAGAAGTAT17580.26830068601341506No Hit
ATATAAATCATCATTTAGCAAGTTGTCATGTGGTAGTACCATACGAGGAC17280.2637221760131861No Hit
GCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCCATCAG16710.25502300701275116No Hit
ATGTAACTGCCGTTTTCCATCAGACCCAGTTCACTCACCGTCCCACAGTA16670.2544125390127206No Hit
CAACGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCCA16240.24785000801239251No Hit
CTCCAATTCCAAACCCAAATGTGCAAATTGGACAGAGACAGGGACTGTCC15550.23731943501186598No Hit
CTATAGTAGATCTATAGAAGTATAAATCTATAAATGAAACCCAATCAGCA15180.23167260601158363No Hit
GATTACAAGAGCATCATAAACATCCAAACACTCTATGTGTACAGACAAGA14900.22739933001136997No Hit
GTTTTACCTGTTTACATTACTAAATAAAAATATGTTTTATAAAACTTTTA14490.22114203301105712No Hit
TTCCAGTATTGTGAATATTTTAGATACGATGCTCACAAGACAAATAAATA14210.21686875701084343No Hit
GCATCAGTGTGGGGTTCTTCTTTCAGACTCGATCTGTCCTATTTATGAGA14020.21396903401069844No Hit
CATGGAAAGAGGGCATCATTTTGTTTGTCTTTGGGGACACTGAAATGACA13740.2096957580104848No Hit
CTTTCCATGTATCTGATGGAGTTTGAACACCATACTCACAGCATCCATAG13610.2077117370103856No Hit
GTAGAAACAACGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCG13600.20755912001037793No Hit
AATCAGCATCGTTCTCAATACTGTTACTCTCTGGTCTGCATCAGGATCAC13570.20710126901035508No Hit
GCTTTCACAGTCCAGAATGGAAAGGAGACAATCACTCCAATTCCAAACCC13560.20694865201034743No Hit
GTTCAAATTTCCTTCTGTAGAACTGCTTTCACAGTCCAGAATGGAAAGGA13550.20679603501033977No Hit
GTTCTCAATACTGTTACTCTCTGGTCTGCATCAGGATCACAAGACAGAAC13400.20450678001022535No Hit
CTGTTACTCTCTGGTCTGCATCAGGATCACAAGACAGAACTCCACTCTGG13220.20175967401008799No Hit
TCCATAGAGTTTGTTGATCCTCCGGATGTCAGTGGTGGACAGTCCCTGTC12870.1964180790098209No Hit
AATCTGACAGATCTATAGTAGATCTATAGAAGTATAAATCTATAAATGAA12650.19306050500965302No Hit
GTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTGGTAACTGTGT12220.18649797400932489No Hit
CTTCAGCACAGGTGGGGCCGTTGTAGGGCAGATCACACAGGCAGGTGAAG11890.18146161300907307No Hit
GTGCTAATCTGACAGATCTATAGTAGATCTATAGAAGTATAAATCTATAA11820.18039329400901968No Hit
TTGTAGGGCAGATCACACAGGCAGGTGAAGCTGGCCACACCATCCACACA11750.17932497500896624No Hit
GTATCAGAGAGCATTCTTCCATCGTGATTCCTGTCATTTCAGTGTCCCCA11690.17840927300892048No Hit
GTCATGTGGTAGTACCATACGAGGACAGCATCAGTGTGGGGTTCTTCTTT11640.1776461880088823No Hit
GTCCCTGTCTCTGTCCAATTTGCACATTTGGGTTTGGAATTGGAGTGATT11580.17673048600883653No Hit
GTGGTAGTACCATACGAGGACAGCATCAGTGTGGGGTTCTTCTTTCAGAC11440.1745938480087297No Hit
GTCCCACAGTATTTGGCAAAGGCACATGTCTTGTGTTTTTAATCAGTCCA11410.1741359970087068No Hit
ACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCCATCAGACCCAGTTC11370.17352552900867627No Hit
GGGATAATGTAACTGCCGTTTTCCATCAGACCCAGTTCACTCACCGTCCC11280.1721519760086076No Hit
GTTTACATTACTAAATAAAAATATGTTTTATAAAACTTTTACAAATCACT11170.17047318900852365No Hit
TAGTAGATCTATAGAAGTATAAATCTATAAATGAAACCCAATCAGCATCG11030.16833655100841682No Hit
CAATACTGTTACTCTCTGGTCTGCATCAGGATCACAAGACAGAACTCCAC11030.16833655100841682No Hit
GCATTATGGGAGGTAAGAAAATGGGACCCACTGAATAAAACGTATTCATC10960.1672682320083634No Hit
TTCCTAGAGTGGCCACTAGGAGGAGTTCCAGCCTCAGTCAGTAATGATCT10880.16604729600830237No Hit
GTCCAGAGTGGAGTTCTGTCTTGTGATCCTGATGCAGACCAGAGAGTAAC10780.16452112600822605No Hit
CAACAGCAAAGAGGGATATTGAGGTCATCTCAGTGATTTATTTTCCAGTA10740.16391065800819554No Hit
CTCTAGGAATAGCCAGTTGGAGAGGTGGTGTTCTCAGGGTAGAGTAGCCT10720.1636054240081803No Hit
AATAAATATAAATCATCATTTAGCAAGTTGTCATGTGGTAGTACCATACG10710.16345280700817263No Hit
GTGAGTATGGTGTTCAAACTCCATCAGATACATGGAAAGAGGGCATCATT10610.16192663700809634No Hit
GTTACTCTCTGGTCTGCATCAGGATCACAAGACAGAACTCCACTCTGGAC10550.16101093500805053No Hit
TTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCCATCAGACCCAGT10490.16009523300800477No Hit
TTATAAAACTTTTACAAATCACTGTTCAAATTTCCTTCTGTAGAACTGCT10120.15444840400772242No Hit
GTACCATACGAGGACAGCATCAGTGTGGGGTTCTTCTTTCAGACTCGATC10000.15261700000763084No Hit
GAGGGATATTGAGGTCATCTCAGTGATTTATTTTCCAGTATTGTGAATAT9890.15093821300754692No Hit
GAGACAGGGACTGTCCACCACTGACATCCGGAGGATCAACAAACTCTATG9840.15017512800750876No Hit
GGATTGAAAGGAAGAAAGAGTGACGGAGCAAAAAAGCGAAAGGGAGAGTG9680.14773325600738665No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG9510.14513876700725695No Hit
AGTAGAAACAACGCACCAACAACCTGAACACTCCATATGACTACACTTCT9490.14483353300724167No Hit
GTAGTAACAAGAGCAACAACAGCAACAACATAACAATGTAAGAGACCTTG9290.14178119300708908No Hit
GCAAAGAGGGATATTGAGGTCATCTCAGTGATTTATTTTCCAGTATTGTG9250.14117072500705855No Hit
GAGTATGGTGTTCAAACTCCATCAGATACATGGAAAGAGGGCATCATTTT8950.1365922150068296No Hit
GTAGAAACAACAGCAAAGAGGGATATTGAGGTCATCTCAGTGATTTATTT8860.13521866200676091No Hit
CACGTGCTAATCTGACAGATCTATAGTAGATCTATAGAAGTATAAATCTA8820.1346081940067304No Hit
CCATACTCACAGCATCCATAGAGTTTGTTGATCCTCCGGATGTCAGTGGT8630.13170847100658542No Hit
ATTGTGAATATTTTAGATACGATGCTCACAAGACAAATAAATATAAATCA8620.1315558540065778No Hit
CTATGGATGCTGTGAGTATGGTGTTCAAACTCCATCAGATACATGGAAAG8550.13048753500652438No Hit
GTTGTCATGTGGTAGTACCATACGAGGACAGCATCAGTGTGGGGTTCTTC8460.1291139820064557No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA8350.12743519500637177No Hit
CTCTATGTGTACAGACAAGATCAACTCTAAATGACTGAGCATTCAGATGC8340.12728257800636414No Hit
ATTTATGAGAAGAAAAACCAATCAGGGTATCAGAGAGCATTCTTCCATCG8230.1256037910062802No Hit
CTTCTGTGATGCATTATGGGAGGTAAGAAAATGGGACCCACTGAATAAAA8010.12224621700611231No Hit
CTAAATGACTGAGCATTCAGATGCTTGCTTTTCTGTGAACGGCCTCAGCT7960.12148313200607416No Hit
TAATGTAACTGCCGTTTTCCATCAGACCCAGTTCACTCACCGTCCCACAG7880.12026219600601311No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCAT7830.11949911100597496No Hit
GTAGTAACAAGAGCATGTGAGATTATTTTTGAATTGACAATTATTTTAAA7820.11934649400596734No Hit
GTATTGTGAATATTTTAGATACGATGCTCACAAGACAAATAAATATAAAT7710.11766770700588339No Hit
GTGGTGGACAGTCCCTGTCTCTGTCCAATTTGCACATTTGGGTTTGGAAT7680.11720985600586048No Hit
ATACTGTTACTCTCTGGTCTGCATCAGGATCACAAGACAGAACTCCACTC7660.11690462200584523No Hit
GTTCTACCCTCAGGCACATGGTCATGTTGAGATCAAGGCTACTCTACCCT7500.11446275000572313No Hit
ACAACAGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTC7450.11369966500568499No Hit
GCATCGTTCTCAATACTGTTACTCTCTGGTCTGCATCAGGATCACAAGAC7420.11324181400566209No Hit
TCTCAGTGATTTATTTTCCAGTATTGTGAATATTTTAGATACGATGCTCA7410.11308919700565447No Hit
TCACAAGACAAATAAATATAAATCATCATTTAGCAAGTTGTCATGTGGTA7330.11186826100559341No Hit
AACAACGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTC7320.11171564400558578No Hit
GTTCAAACTCCATCAGATACATGGAAAGAGGGCATCATTTTGTTTGTCTT7300.11141041000557052No Hit
AACCCAATCAGCATCGTTCTCAATACTGTTACTCTCTGGTCTGCATCAGG7270.11095255900554764No Hit
ATCAGACCCAGTTCACTCACCGTCCCACAGTATTTGGCAAAGGCACATGT7240.11049470800552474No Hit
AATGAAACCCAATCAGCATCGTTCTCAATACTGTTACTCTCTGGTCTGCA7140.10896853800544844No Hit
GGAGACAATCACTCCAATTCCAAACCCAAATGTGCAAATTGGACAGAGAC7060.10774760200538738No Hit
GAGCTAATCTGACAGATCTATAGTAGATCTATAGAAGTATAAATCTATAA7030.10728975100536449No Hit
GGCACATGTCTTGTGTTTTTAATCAGTCCAGAGTGGAGTTCTGTCTTGTG7000.1068319000053416No Hit
GTAGAAACAACAGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCC6950.10606881500530344No Hit
GTAGAACTGCTTTCACAGTCCAGAATGGAAAGGAGACAATCACTCCAATT6940.10591619800529581No Hit
GTAGTACCATACGAGGACAGCATCAGTGTGGGGTTCTTCTTTCAGACTCG6820.10408479400520423No Hit
TGACTACACTTCTGTGATGCATTATGGGAGGTAAGAAAATGGGACCCACT6810.10393217700519662No Hit
CCATCAGACCCAGTTCACTCACCGTCCCACAGTATTTGGCAAAGGCACAT6790.10362694300518134No Hit
ATTTTAGATACGATGCTCACAAGACAAATAAATATAAATCATCATTTAGC6650.10149030500507451No Hit
ACTCTGGACTGATTAAAAACACAAGACATGTGCCTTTGCCAAATACTGTG6590.10057460300502873No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTT100.007090944144.99565145
GTAGACG100.007090944144.995659
GCTAAGT100.007090944144.995658
GGACGGG301.101489E-7120.82971145
CTCCTAA256.4641627E-6115.99651145
CCCAAGC1000.0101.589991
AAGCAGC1100.098.936184
AGCCACT1100.098.860678
TGAAAGG2350.083.346265
CCTTAGA607.986637E-872.553192
TAACACG809.822543E-1172.5421145
GAGGGCT608.029019E-872.4978267
CAAGCAG1500.067.716323
CTTAGAG651.3902172E-766.9721763
AACAACG23550.065.879137
ACGCACG350.00355560862.188454
TTAGAGG702.321467E-762.188454
TCCAACC350.00355560862.188452
CAGAGCG350.003557760362.1789555
GTTGTCC350.003566378562.140999
Produced by FastQC (version 0.11.5)