FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_9839_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9839_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272095
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA6960.2557930134695603No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC6500.23888715338392844No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT6160.2263915176684614No Hit
AGCACGCACTTTCTTAAAATGTCACTGTTTGGAGACACAATTGCCTACTT5660.20801558279277457No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC5510.20250280233006854No Hit
TCATAATCCTCTGCTGTGTCCCTCCCAAAGAAGAAATTGGGGATGGTCTG5350.19662250316984878No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA5070.18633197963946416No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT4610.1694261195538323No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA4490.16501589518366747No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT4250.1561954464433378No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT4130.15178522207317297No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG4110.15105018467814552No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG3980.14627244161046693No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA3930.14443484812289825No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCGAT3600.13230673110494498No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA3570.13120417501240378No Hit
ATCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG3320.12201620757456035No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA3310.12164868887704662No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCC3160.11613590841434059No Hit
GGAAAATACAGGGCTTAACTTTCCTTATAGACAAATGGAGCAAGAAACAT3130.11503335232179938No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT3050.11209320274168948No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT3050.11209320274168948No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT3040.11172568404417575No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA3020.11099064664914828No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT3010.11062312795163455No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA3010.11062312795163455No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAGCA2980.10952057185909334No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTT2970.1091530531615796No Hit
GTAAGAAATACAGTAAAATTAGATTTAATGCAATTCTTCTATCTCCAAAA2910.10694794097649718No Hit
CTACAATAAAGGAAAATACAGGGCTTAACTTTCCTTATAGACAAATGGAG2900.10658042227898344No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT2880.10584538488395596No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC2780.10217019790881861No Hit
TTCTTATAGGGCTGAAATAGATGGTTTTATCATCTCTTACCATCTTGTAA2780.10217019790881861No Hit
ATATTGAGGGGCGTGATGCAGATGTCAAAGGAAATCTACTCAAGATGATG2740.10070012311876367No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGGA100.0070880456145.01
CACAGGA100.0070880456145.03
ACGTGCA400.0145.02
CACGTGC550.0131.818191
ACGCACT700.0113.928574
CGCACTT700.0113.928575
CACGCAC650.0111.538473
GCACGCA650.0111.538472
AGCACGC700.0103.571431
GAGAATA501.9463187E-10101.59
GAAACAC301.5973568E-596.6666644
GTAACAA2100.089.76194
TGAGAAT608.258212E-1084.5833368
AAACACG353.4327713E-582.857145
GGCGAAA353.4327713E-582.85714145
TAGAAAC754.836693E-967.666672
TAGTAAC2950.063.8983042
CCATCCA502.0076321E-458.09
TAGTACC400.00604617154.3754
TAACAAG3500.053.8571435
Produced by FastQC (version 0.11.5)