FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_9839_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9839_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946361
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC46400.23839359707680127No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA46110.23690363709507126No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT44060.22637116136215224No Hit
AGCACGCACTTTCTTAAAATGTCACTGTTTGGAGACACAATTGCCTACTT40090.20597412299157247No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC39290.20186388855921383No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA35920.18454952601290306No Hit
TCATAATCCTCTGCTGTGTCCCTCCCAAAGAAGAAATTGGGGATGGTCTG35720.18352196740481339No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA31570.16220012628695293No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT31490.1617891028437171No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA30960.15906607253227947No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG29320.15064009194594427No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT29080.14940702161623665No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT28870.1483280850777425No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG27990.143806827202148No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA26210.13466155559015003No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCGAT25280.1298834080625331No Hit
ATCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG24500.12587592949098342No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA23420.12032711300729926No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT22850.11739857097424373No Hit
GGAAAATACAGGGCTTAACTTTCCTTATAGACAAATGGAGCAAGAAACAT22340.1147782965236151No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT22090.11349384826350303No Hit
GAATAAACACCCACATTCCAAACGTATTGAAACAATCACAGTCATAGTGT21680.11138735311691922No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCC21670.11133597518651474No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTT21550.11071944002166094No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA21340.10964050348316678No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT20920.10748263040617849No Hit
TTCTTATAGGGCTGAAATAGATGGTTTTATCATCTCTTACCATCTTGTAA20750.10660920558930229No Hit
CTACAATAAAGGAAAATACAGGGCTTAACTTTCCTTATAGACAAATGGAG20500.10532475732919022No Hit
GTAAGAAATACAGTAAAATTAGATTTAATGCAATTCTTCTATCTCCAAAA20460.10511924560757228No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA20120.10337239597381986No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAGCA20040.10296137253058399No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG19730.10136865668804504No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT19700.10121452289683158No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT19620.10080349945359572No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTAAC100.007081883145.064971
CCGGTCT100.00708406145.050064
TGTCGGT100.00708406145.050062
CGCACTG100.007085693145.038886
CGTGCTC301.10219844E-7120.83158145
GCACGCA5100.0119.452992
CACGCAC5200.0118.550543
AGCACGC5150.0118.305421
GTAACAA15800.0109.246564
GAAACAC4400.0107.139254
ACGCACT6000.0103.952544
CGCACTT6000.0103.944535
AAACACG4800.098.2034155
CGGTGCA451.08957465E-896.700042
ACACGTG1750.091.141537
GAGAATA3350.090.89429
GGAGCGT406.1098945E-790.64936
CGGAGCG406.1098945E-790.64935
CGAGCTG259.3752274E-487.030043
AACACGT1850.086.2393346
Produced by FastQC (version 0.11.5)