FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_9825_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9825_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2182336
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT124880.5722308572098889No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA88840.40708671808557434No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA85220.39049898824012436No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC84650.387887108126338No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC68150.31228005220094435No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT59140.2709940174198657No Hit
TTCTAATACTTTACTTCCCTCCTTTTCAAAACCCAACCATTCATTGAACC58910.2699401008827238No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG58240.2668699961875715No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG53970.24730380656324236No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC51810.23740615560573622No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA51710.2369479310243702No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT51510.2360314818616382No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT48870.22393435291357516No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG48220.22095589313469605No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG48200.22086424821842282No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT48200.22086424821842282No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT44250.20276437725446494No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGA40460.18539766562069268No Hit
ATCTAGAGGTTTGCTATGTAATAAGTGACATGAATTTTCTTGACGAAGAA39980.1831981876301358No Hit
GTATTACCCAAGGGTTGTTACTGATACATTCTTCTATTCCAGAATACATT38630.17701215578169446No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT36630.16784766415437405No Hit
GTCATAATGGATACTTTTATTACAAGAAACTTCCAGACTACAATAATACA36300.16633552303586616No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT35880.16441097979412886No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA35850.16427351241971905No Hit
GTAATAAGTGACATGAATTTTCTTGACGAAGAAGGAAAAGCATATACAGC35670.1634487081732602No Hit
CTTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC35280.16166163230593272No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAGCA35120.16092847297574708No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG33500.15350523475761751No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT33130.15180980380656325No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT31680.1451655473767559No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGACTTGCCCCAAGGGA30590.14017089943986627No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA29310.13430562479838118No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT29030.13302259597055632No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC28560.13086894043813602No Hit
ATAATAAACAGTGAAGATGAGCATCTATTGGCACTTGAGAGAAAACTAAA28550.1308231179799994No Hit
CTGTATTACCCAAGGGTTGTTACTGATACATTCTTCTATTCCAGAATACA28330.12981502390099417No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC28140.12894439719639872No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTATCACATGTAT26500.12142951406199595No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA26480.12133786914572274No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA26320.12060470981553713No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT26070.11945914836212206No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT25850.1184510542831168No Hit
GTACTAAATTGAGTACTATGTCTTTTATTCATCCATTATTTCATCTACTG25530.11698473562274554No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT24570.11258577964163173No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA24470.1121275550602657No Hit
CCATATAAACAATAAAAATAGCTAGCATTAATGTTACAGCCAAACTAGAA23640.10832429103492772No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT23390.10717872958151266No Hit
CCTTTAATGCAGGAGAATTTTCTCTCCCCACTTTTGACTCATTGAACATT23190.10626228041878061No Hit
CTATAAGCTCACAAATAGAACTTGCAGTCTTGCTTTCCAACGAAGGAATA23180.10621645796064401No Hit
GTCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACTTTACTTCC23160.1061248130443708No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT22910.10497925159095574No Hit
AGCATATACAGCATTAGAAGGACAAGGGAAAGAACAAAATTTGAGACCAC22490.10305470834921844No Hit
ATTCAACATCTGTGTCCATCTAGAGGTTTGCTATGTAATAAGTGACATGA22430.10277977360039885No Hit
CCCCTAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGA22350.10241319393530601No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG22320.10227572656089622No Hit
ATTGAGTACTATGTCTTTTATTCATCCATTATTTCATCTACTGATTCTAA22300.102184081644623No Hit
ATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTAAT22020.10090105281679815No Hit
TTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTATACTG21980.10071776298425172No Hit
CCTCAGAGCTGACACTATAAGCTCACAAATAGAACTTGCAGTCTTGCTTT21860.10016789348661251No Hit
AGTATGCACTCTGTATTACCCAAGGGTTGTTACTGATACATTCTTCTATT21830.1000304261122027No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTAGT100.0070819533145.06491
GTGCAAC100.007087293145.028326
GGGGCTC100.007087293145.028326
ACGCGTT100.0070916642144.99841145
AGCACGC15100.0128.252731
GCACGCA15250.0126.9621352
CACGCAC15350.0126.135023
CGGTGCA1100.0125.25462
GGTGCAT1250.0110.224053
GAAACAC7800.090.179934
ACGTGCA3350.086.586062
ACGCACT23150.086.4551544
CGCACTT23300.085.8985755
AAACACG8250.085.2610245
TAGTAAC43000.085.163942
AAGCACG1800.080.5916061
CCCGTAC755.638867E-1177.350213
ACAACGC7450.074.932078
CACTTTC32300.072.723667
GAGCAGA1700.072.532451
Produced by FastQC (version 0.11.5)