FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_9825_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9825_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2193303
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT125350.5715124631662839No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA89280.4070573012483912No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA85530.38995980035590155No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC85130.38813606692736935No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC68500.3123143496361424No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT59290.2703228874441881No Hit
TTCTAATACTTTACTTCCCTCCTTTTCAAAACCCAACCATTCATTGAACC59130.26959339407277516No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG58540.26690338726569013No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG54220.24720706623754218No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC52070.2374044990591815No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA51990.23703975237347508No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT51710.23576313897350254No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT49090.2238176850166165No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT48460.22094530486667824No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG48430.22080852485953834No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG48410.22071733818811173No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT44380.2023432238956496No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGA40660.1853825030103No Hit
ATCTAGAGGTTTGCTATGTAATAAGTGACATGAATTTTCTTGACGAAGAA40180.18319402289606132No Hit
GTATTACCCAAGGGTTGTTACTGATACATTCTTCTATTCCAGAATACATT38810.17694773590333848No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT36840.1679658487678173No Hit
GTCATAATGGATACTTTTATTACAAGAAACTTCCAGACTACAATAATACA36500.1664156753535649No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT36110.164637535260746No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA36040.16431838191075288No Hit
GTAATAAGTGACATGAATTTTCTTGACGAAGAAGGAAAAGCATATACAGC35870.16354329520362668No Hit
CTTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC35430.16153718843224124No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAGCA35290.16089888173225497No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG33660.15346716801098617No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT33320.15191699459673377No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT31830.14512358757545127No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGACTTGCCCCAAGGGA30750.1401995073184143No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA29400.13404440699711803No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT29150.1329045736042854No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC28710.13089846683289996No Hit
ATAATAAACAGTGAAGATGAGCATCTATTGGCACTTGAGAGAAAACTAAA28680.13076168682576006No Hit
CTGTATTACCCAAGGGTTGTTACTGATACATTCTTCTATTCCAGAATACA28450.12971304010435403No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC28360.1293027000829343No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTATCACATGTAT26620.12136945966881912No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA26590.12123267966167922No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA26490.12077674630454616No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT26200.11945453956886029No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT26030.1186794528617341No Hit
GTACTAAATTGAGTACTATGTCTTTTATTCATCCATTATTTCATCTACTG25620.11681012609748857No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT24670.11247875920472455No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA24610.11220519919044472No Hit
CCATATAAACAATAAAAATAGCTAGCATTAATGTTACAGCCAAACTAGAA23730.10819298564767384No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT23520.10723552559769443No Hit
CTATAAGCTCACAAATAGAACTTGCAGTCTTGCTTTCCAACGAAGGAATA23290.10618687887628842No Hit
CCTTTAATGCAGGAGAATTTTCTCTCCCCACTTTTGACTCATTGAACATT23260.1060500988691485No Hit
GTCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACTTTACTTCC23250.10600450553343518No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT23030.10500145214774248No Hit
AGCATATACAGCATTAGAAGGACAAGGGAAAGAACAAAATTTGAGACCAC22550.10281297203350381No Hit
ATTCAACATCTGTGTCCATCTAGAGGTTTGCTATGTAATAAGTGACATGA22510.10263059869065058No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG22450.10235703867637075No Hit
CCCCTAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGA22430.10226585200494415No Hit
ATTGAGTACTATGTCTTTTATTCATCCATTATTTCATCTACTGATTCTAA22380.10203788532637761No Hit
ATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTAAT22120.10085245859783167No Hit
TTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTATACTG22100.10076127192640506No Hit
AGTATGCACTCTGTATTACCCAAGGGTTGTTACTGATACATTCTTCTATT21950.10007737189070547No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT100.007085436145.041064
GGCGAGC100.007085436145.041063
CGTAGAG100.007086885145.031136
AGCACGC15950.0125.9763261
CACGCAC16150.0124.834083
GCACGCA16150.0124.834082
CCCGTAC800.0108.780793
CGGTGCA700.0103.600752
CGTACAG850.0102.379585
CCGTACA900.096.694044
GCCCGTA900.096.694042
GTACAGG900.096.6874166
GGTGCAT800.090.650663
TAGTAAC43300.089.43642
ACAACGC7150.089.2295768
TAGCCCA259.377701E-487.0246354
ACGCACT23750.086.4139254
CAACGCA7400.086.2150659
CGCACTT23650.086.164075
GAGCAGA1350.085.971821
Produced by FastQC (version 0.11.5)