FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_9823_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9823_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316024
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG37070.2816817930372091No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT23740.18039184695719834No Hit
GTCCTTGAGAGTGTTGGTAAACGGAACATTCCTCAAACACCCCAATGGAT23160.17598463249910337No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA21860.16610639319647666No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC19740.14999726448757775No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACTGAT18950.14399433444982768No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCACTTGAAAGAAAGCTGAA18170.13806739086825165No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA17870.13578779718303008No Hit
GTTTATTATTCCTTCATTGGAAAGCAGGACTGCGAGTTCTATTTGTGAGC16500.12537765268718504No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT15550.11815893935065015No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC15150.11511948110368808No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT14710.11177607703202982No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG14590.11086423955794118No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG14510.11025634790854878No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA14340.10896457815358991No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT14330.10888859169741584No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA14240.1082047135918494No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC13890.10554518762575758No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT13860.10531722825723544No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCAT13610.10341756685288414No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG13320.10121395962383664No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT13250.10068205443061828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC1950.0122.728331
CACGCAC2000.0119.655573
GCACGCA2050.0116.7371442
GAGCAGA650.0111.5712051
CGGTGCA600.0108.7777942
AGAGCCG301.597921E-596.68775
GTAACAA9350.096.17434
GCAAGCG259.387803E-486.99909145
ACGTGCA1350.080.576142
CAATTTA554.3888576E-879.1081165
ACGCACT3650.075.498754
CGCACTT3300.074.713225
CGTGCTC300.001933917272.518533
GTGCTCC300.001933917272.518534
CAGCGGC502.3067896E-672.499247
AGAGTAT3000.072.499248
GAAACAC3650.067.5515064
AAACACG3650.067.548945
GCATAGC651.395565E-766.9427261
AGTCAAT651.3959288E-766.940182
Produced by FastQC (version 0.11.5)