FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n01_SM2_9752_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9752_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1175604
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA40950.348331581042596No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT31880.27117975100458996No Hit
CCTTAGACCAGTCATTGTCAAAATGTCGTTCAGAGTAAGAGAATCGCGCT29070.2472771443445242No Hit
CCATTAGGCAATACTCTTCCCCTCTGCTACCTGTGCACTCCAGCTGCCTC28720.24429995134416013No Hit
GTCTATGATTGTGTATGATAACAATGAGTCACTCTTTTGGACATGGAGGA28240.24021694380080366No Hit
CTCTTATCCTCTCTCTCTCCCACTCTGTCTCTCATCCTCTCTCTCTCCCA28060.238685815972045No Hit
CAATATACTAGGGGAAAGAAGTAAAAGGACAGCGAGGGGAAATGTGTACT22540.191731229223446No Hit
ATATAACGCAGTATAATCAAAACAACTGACTCTTGCCCCGAATCTGCCAT20300.17267719402111595No Hit
GGTACTGACAGAGGAACGATGGAGACGGAGAGACAATATACTAGGGGAAA19970.1698701263350584No Hit
GGGCTAATCCTGTGCATGCAGCACGACACGAAGAACACCGACAAACTGGA18530.1576211037049891No Hit
GACAATATACTAGGGGAAAGAAGTAAAAGGACAGCGAGGGGAAATGTGTA18510.15745097839068256No Hit
CTCTATAATCTGCGCTGGAAATCGGTGGCCTACACGCCGCACCGCTCCAA18490.15728085307637607No Hit
GTATTCATCATCTTTGAGCAAATTCAGCTCTCGGAGAGGAAGGAAAAGCA18480.1571957904192228No Hit
GTATTACTGTCTATGATTGTGTATGATAACAATGAGTCACTCTTTTGGAC18100.15396340944739897No Hit
ATACTAGGGGAAAGAAGTAAAAGGACAGCGAGGGGAAATGTGTACTTTAA18060.1536231588187859No Hit
GTACATGGACACCGGGCTGGTGCGGTTCGGAGAGCCGCCGCAGGAACCGA17450.1484343367324371No Hit
ATATACTAGGGGAAAGAAGTAAAAGGACAGCGAGGGGAAATGTGTACTTT16720.1422247627602492No Hit
CTCTTGTGGTGTTTATGCAAAAGCCGGTCACGTGAAGTCTGCAAATGTAC15660.13320812110200372No Hit
GTATAGTGTTGAGTGAGAGTGCAGGCATTACTCCGATAAATAAAGAATGA15650.13312305844485048No Hit
GGGAAGAGAGGAAGAGAAAAAGAATGGAAAGAAAAAGTGAGTGAAATGGT15470.1315919306160918No Hit
CTCCCACTCTGTCTCTCATTCTCTCTCTCCATCCCATTCTCTCTCTCTCT15310.13023092810163966No Hit
GTGGGAAGGAGAGGAGGGACAGGGAAGAGAGGAAGAGAAAAAGAATGGAA15040.12793423635850165No Hit
CTATCATTCATGTCTTGGGTCCTCAAAGGACCACCGGTTCAAACACATTT15040.12793423635850165No Hit
CTCCCACTCTGTCTCTCATCCTCTCTCTCTCCCACTCTGTCTCTCATTCT14810.1259777952439767No Hit
GTATAATCAAAACAACTGACTCTTGCCCCGAATCTGCCATGAGATTTCAT14800.12589273258682346No Hit
GATATAACGCAGTATAATCAAAACAACTGACTCTTGCCCCGAATCTGCCA14530.12359604084368546No Hit
GGACAGGGAAGAGAGGAAGAGAAAAAGAATGGAAAGAAAAAGTGAGTGAA14380.12232010098638657No Hit
GGACAAGACAGTGTAACCAGTCAGAGCAGTCGCAGAGCAGGGACATAAGC14300.12163959972916051No Hit
GGAGAGGAGGGACAGGGAAGAGAGGAAGAGAAAAAGAATGGAAAGAAAAA14060.11959809595748228No Hit
GTATATCAGAAATCAATGATATCGATGTAGCACAGACACGCTGACCCTGA13950.11866240672879644No Hit
GCTCTACATCACTGAAGGAGACAGTGACGAGGTGAACAACGATCATGAAG13420.1141540858996737No Hit
TTCCAGCGCTGCGTGGACCAGATCCCTGAGCATCTCTCCGACGTCCCCCT12840.10922045178478466No Hit
ATACAGCAGTGTGCAAACAACACCAAGGCTTCAATTACCCAACAAGCACA12830.10913538912763142No Hit
CTCCCTCTCTTATCCTCTCTCTCTCCCACTCTGTCTCTCATCCTCTCTCT12690.10794451192748579No Hit
GTTCTAGTGTCACCAACATAAACTTTCTCTCCAAATAAACAGTAAAAGAA12600.10717894801310646No Hit
GTCTGTGATGTATTATCACACCACAGGCGGCACATTGTATATCAGAAATC12570.10692376004164668No Hit
CTTTTAATCATGCTCCATGCAGGTACACATACCGACTGGCTCTACAAGGT12570.10692376004164668No Hit
AAATGGTACTGACAGAGGAACGATGGAGACGGAGAGACAATATACTAGGG12130.10318100312690327No Hit
TTATTACACACTCTATAATCTGCGCTGGAAATCGGTGGCCTACACGCCGC11960.10173493795529788No Hit
ATGTAGCACAGACACGCTGACCCTGAAGAGCAGCTTGTTTATTCTCAGCA11830.1006291234123055No Hit
GTATAAGCCTGTCTGTCCACTCCACCTCAGCTGTATCATTTCTGAAGGGG11790.10028887278369246No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG100.007083107145.054171
GTACGGG256.453356E-6116.043341
TACGGGA256.4588185E-6116.023592
GCGTCGG451.09066605E-896.6822056
TCCGCAC259.38751E-486.99918145
TTCTAGT2450.082.8739852
GGCGTCG554.390131E-879.103625
TAGCTGG702.8103386E-972.511666
TGTCACC2900.069.9993448
GGACAGT1400.067.332256
TAGCGCT1200.066.471854
CGAAAGC30000.064.054683
GTTCTAG3200.063.4611971
TAGCATG2000.058.0117954
GCGGCCG502.008793E-458.0093275
GCGAAAG33200.057.880742
CTCGCGG1400.056.9637539
AGCGAAA34500.055.4989851
TCCTACG400.006051374654.3744858
TGGTCCG400.006051374654.3744859
Produced by FastQC (version 0.11.5)