FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_9752_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9752_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1181438
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA41130.34813506929690763No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT32010.27094100579124764No Hit
CCTTAGACCAGTCATTGTCAAAATGTCGTTCAGAGTAAGAGAATCGCGCT29260.24766428708065932No Hit
CCATTAGGCAATACTCTTCCCCTCTGCTACCTGTGCACTCCAGCTGCCTC28900.2446171529949096No Hit
GTCTATGATTGTGTATGATAACAATGAGTCACTCTTTTGGACATGGAGGA28400.24038502232025719No Hit
CTCTTATCCTCTCTCTCTCCCACTCTGTCTCTCATCCTCTCTCTCTCCCA28190.23860752743690314No Hit
CAATATACTAGGGGAAAGAAGTAAAAGGACAGCGAGGGGAAATGTGTACT22690.1920540900157266No Hit
ATATAACGCAGTATAATCAAAACAACTGACTCTTGCCCCGAATCTGCCAT20380.17250164629883244No Hit
GGTACTGACAGAGGAACGATGGAGACGGAGAGACAATATACTAGGGGAAA20060.1697930826670549No Hit
CTCTATAATCTGCGCTGGAAATCGGTGGCCTACACGCCGCACCGCTCCAA18630.157689188937549No Hit
GGGCTAATCCTGTGCATGCAGCACGACACGAAGAACACCGACAAACTGGA18620.15760454632405593No Hit
GTATTCATCATCTTTGAGCAAATTCAGCTCTCGGAGAGGAAGGAAAAGCA18570.1571813332565907No Hit
GACAATATACTAGGGGAAAGAAGTAAAAGGACAGCGAGGGGAAATGTGTA18540.15692740541611155No Hit
GTATTACTGTCTATGATTGTGTATGATAACAATGAGTCACTCTTTTGGAC18170.15379562871686878No Hit
ATACTAGGGGAAAGAAGTAAAAGGACAGCGAGGGGAAATGTGTACTTTAA18160.15371098610337572No Hit
GTACATGGACACCGGGCTGGTGCGGTTCGGAGAGCCGCCGCAGGAACCGA17550.14854778668029978No Hit
ATATACTAGGGGAAAGAAGTAAAAGGACAGCGAGGGGAAATGTGTACTTT16770.14194566282784202No Hit
GTATAGTGTTGAGTGAGAGTGCAGGCATTACTCCGATAAATAAAGAATGA15710.1329735457975789No Hit
CTCTTGTGGTGTTTATGCAAAAGCCGGTCACGTGAAGTCTGCAAATGTAC15710.1329735457975789No Hit
GGGAAGAGAGGAAGAGAAAAAGAATGGAAAGAAAAAGTGAGTGAAATGGT15500.13119605091422487No Hit
CTCCCACTCTGTCTCTCATTCTCTCTCTCCATCCCATTCTCTCTCTCTCT15350.12992641171182914No Hit
GTGGGAAGGAGAGGAGGGACAGGGAAGAGAGGAAGAGAAAAAGAATGGAA15130.12806427421498207No Hit
CTATCATTCATGTCTTGGGTCCTCAAAGGACCACCGGTTCAAACACATTT15100.12781034637450295No Hit
CTCCCACTCTGTCTCTCATCCTCTCTCTCTCCCACTCTGTCTCTCATTCT14870.12586356626416284No Hit
GTATAATCAAAACAACTGACTCTTGCCCCGAATCTGCCATGAGATTTCAT14840.12560963842368367No Hit
GATATAACGCAGTATAATCAAAACAACTGACTCTTGCCCCGAATCTGCCA14640.1239167861538227No Hit
GGACAGGGAAGAGAGGAAGAGAAAAAGAATGGAAAGAAAAAGTGAGTGAA14410.1219700060434826No Hit
GGACAAGACAGTGTAACCAGTCAGAGCAGTCGCAGAGCAGGGACATAAGC14330.12129286513553822No Hit
GGAGAGGAGGGACAGGGAAGAGAGGAAGAGAAAAAGAATGGAAAGAAAAA14140.11968465547917029No Hit
GTATATCAGAAATCAATGATATCGATGTAGCACAGACACGCTGACCCTGA14000.11849965889026762No Hit
GCTCTACATCACTGAAGGAGACAGTGACGAGGTGAACAACGATCATGAAG13500.11426752821561521No Hit
ATACAGCAGTGTGCAAACAACACCAAGGCTTCAATTACCCAACAAGCACA12920.1093582566330184No Hit
TTCCAGCGCTGCGTGGACCAGATCCCTGAGCATCTCTCCGACGTCCCCCT12890.10910432879253926No Hit
CTCCCTCTCTTATCCTCTCTCTCTCCCACTCTGTCTCTCATCCTCTCTCT12740.10783468959014353No Hit
GTTCTAGTGTCACCAACATAAACTTTCTCTCCAAATAAACAGTAAAAGAA12660.10715754868219915No Hit
GTCTGTGATGTATTATCACACCACAGGCGGCACATTGTATATCAGAAATC12620.10681897822822696No Hit
CTTTTAATCATGCTCCATGCAGGTACACATACCGACTGGCTCTACAAGGT12620.10681897822822696No Hit
AAATGGTACTGACAGAGGAACGATGGAGACGGAGAGACAATATACTAGGG12190.10317934584802588No Hit
TTATTACACACTCTATAATCTGCGCTGGAAATCGGTGGCCTACACGCCGC12030.1018250640321371No Hit
ATGTAGCACAGACACGCTGACCCTGAAGAGCAGCTTGTTTATTCTCAGCA11890.10064006744323443No Hit
ACAATATACTAGGGGAAAGAAGTAAAAGGACAGCGAGGGGAAATGTGTAC11860.1003861396027553No Hit
GTATAAGCCTGTCTGTCCACTCCACCTCAGCTGTATCATTTCTGAAGGGG11830.10013221176227614No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTAG151.2288774E-4145.035062
CCGCGTC100.007091284144.99821145
TAGTAGA300.00193397472.517533
GATAGCC300.001935923572.4991157
TAGCATG1650.070.320024
CGAAAGC27450.070.007813
GGACAGT1150.069.361666
TTAGGCT554.0562354E-665.925024
GCGTCGG451.1927704E-464.457296
GCGAAAG30850.062.2922062
TTCTAGT2150.060.7123452
AGCGAAA32700.058.7854461
CAGTGTA4750.056.4729929
GGGCTAA6300.055.2678261
TAGCGCT1051.1077645E-955.2514464
CTCGCGG1051.1095835E-955.237429
AGGACGA400.00604537354.388144
TCTACTA400.00604537354.388145
CTACTAA400.006046385554.385846
TGTCACC2400.054.3743368
Produced by FastQC (version 0.11.5)