FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n01_SM2_9730_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n01_SM2_9730_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1510698
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT88800.5878077550906932No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC46960.3108496867011143No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACTTCCAAAA44610.2952939634526557No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG38690.2561067797799428No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT31750.21016775027172868No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACG31030.205401741446669No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA30360.20096670545668294No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC27050.17905630377481138No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC26980.17859294180570837No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT25220.16694269801111805No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG25180.16667791974305915No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTCTATCACATGTA24930.16502305556769123No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT23440.1551600650824983No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT23170.15337281177310091No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG21400.14165637341149587No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA20960.1387438124628483No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT20680.13689036458643622No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT19430.12861604370959648No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAGATTCCTTCAT19320.1278879034724346No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT18650.1234528674824485No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC18180.12034172283275678No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA17550.11617146511082957No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG17110.11325890416218197No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC16930.11206740195591705No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTGTGAGTTCCAGACACTGCA16910.11193501282188764No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT16800.11120687258472574No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT16660.1102801486465197No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT16170.10703661486279853No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA16090.1065070583266808No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAACTGTGT15950.10558033438847474No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT15430.10213821690370942No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACAGG100.007079749145.078522
AGCACGC11200.0128.899521
GCACGCA11350.0127.183372
CACGCAC11400.0126.625553
AAACACG4850.0101.687695
ACGCACT14500.0101.554974
GAAACAC4950.0101.115334
CGCACTT14600.0100.84275
ACGTGCA1950.096.719022
AACACGT1700.093.855686
GTAACAA22850.092.380424
CGGTGCA1050.089.810522
ATAAGCC259.3884824E-486.99813145
CGGTGCC1100.079.1337362
ACAACGC3050.078.440948
CAACGCA3050.078.440949
ACACGTG1950.078.075257
GCGGTGC2800.077.7283551
GAAGCGG2000.076.173791
GGTGCAT1250.075.4408343
Produced by FastQC (version 0.11.5)